PCR methods in foods

Ebook "How to reliably test for GMOs" have many years of experience in GMO testing, development of real-time PCR methods, implementation of quality system requirements, validations and verification of methods, and measurement uncertainty. The National Institute of Biology is a highly qualified research laboratory and a National Reference Laboratory, which also performs routine analyses of food, feed and seed.

110p nhanphanguyet 28-01-2024 5 2   Download

Part 1 book "PCR methods in foods" includes content: PCR basics, the mythology of PCR - A warning to the wise, sample preparation for PCR, making PCR a normal routine of the food microbiology lab.

73p oursky06 17-10-2023 5 1   Download

Part 2 book "PCR methods in foods" includes content: Molecular detection of foodborne bacterial pathogens, molecular approaches for the detection of foodborne viral pathogens, molecular tools for the identification of foodborne parasites.

78p oursky06 17-10-2023 2 1   Download

Part 2 book "Real-Time PCR in food science - Current technology and applications" includes content: Real time PCR detection of food borne and water borne parasites, real time PCR analysis of genetically modified organisms, real time PCR analysis of food allergens and gluten, real time PCR methods for identification of animal or plant species,... and other contents.

162p oursky05 20-09-2023 6 2   Download

Part 1 book "Real-Time PCR in food science - Current technology and applications" includes content: Introduction to the real time polymerase chain reaction, current challenges in real time PCR diagnostics in food science, sample preparation for real time PCR in food science, standardization of real time PCR methods in food microbiology... and other contents.

135p oursky05 20-09-2023 5 1   Download

fter Salmonella strains were isolated from food samples collected randomly at conventional markets in several districts in Ho Chi Minh city, we evaluated antibiotic resistance by Kirby-Bauer methods, and the serotype name was assigned according to ISO/TR 6579-3:2014 and class 1 integron was investigated by PCR technique.

11p viporsche 03-11-2022 17 5   Download

In the past decades, Lactobacillus species have been widely used in the market because they are thought to have biological properties and beneficial health effects. Conventional microbiological methods are often time consuming and labor-intensive. Real-time polymerase chain reaction (PCR) method will allow to identify and quantify rapidly Lactobacilli probiotics in food. In this study, the thermal shock protein target region (hsp60) was used to identify Lactobacillus acidophilus species. The limit of detection, specificity, and accuracy were of 104 , 100 %, 100 %, respectively.

6p kethamoi9 01-12-2020 24 1   Download

Food authenticity is currently an issue of major concern for food authorities, since incorrect labelling of animal foods may have remarkable negative consequences. To circumvent this problem, DNA based method had been utilised. The present study was carried out for the detection of meat species by using variability in ATPase Subunit 6 and 8 genes by multiplex PCR.

7p nguaconbaynhay7 15-08-2020 14 2   Download

Salmonellosis is one of the most common causal food borne disease in India. An in-house loop-mediated isothermal amplification (LAMP) reaction was established and evaluated for sensitivity and specificity in detecting the presence of Salmonella spp. isolates in foods of animal origin from Mumbai, India. The aim of this study was to develop in-house LAMP for simple and inexpensive detection of Salmonella spp. in animal origin foods using specifically designed primers targeting invA gene contains sequences unique to this genus. The reaction was optimized using genomic DNA of S.

10p angicungduoc6 20-07-2020 23 1   Download

Fabaceae is one of the most diversified and complex family of flowering plants. The important pulses and the medicinally important plant species under this family have a high market value. Now a day, adulteration in the food and herbal medicinal products has become a severe problem. Adulteration of therapeutic herbs and major pulses with related or conflicting species has proved to be hazardous to human health in several cases. We have projected here, a PCR-based method using some of the major universal DNA barcode primers from the plastid region to address this problem.

8p quenchua6 15-06-2020 12 1   Download

A study was conducted to identify the presence of Campylobacter jejuni in chicken meat by polymerase chain reaction. Campylobacteriosis is a food-borne bacterial illness due to the consumption of poultry meats and its by-products. Contaminated raw or undercooked poultry meats and/or by-products are particularly important to cause food-borne. Due to improper slaughtering method, carcass may get contaminated with intestinal contents.

4p caygaocaolon5 27-05-2020 14 0   Download

Melanocortin-4 receptor is part of the central melanocortinergic system and plays critical roles in central regulation of food intake, energy homeostasis and body weight, so that this gene is related to obesity and insulin resistance including single nucleotide polymorphism rs12970134. Thus, this study aimed to find out a protocol for genotyping rs12970134 near Melanocortin-4 receptor by AS-PCR method, analyzing the genotype and allele ratios of this single nucleotide polymorphism in 200 3-5 years old children in Hanoi, Vietnam (50% boys).

7p caygaocaolon5 19-05-2020 21 1   Download

Campylobacteriosis is a food-borne bacterial illness due to the consumption of poultry meats and its by-products. Due to improper slaughtering method, carcass may get contaminated with intestinal contents. A study was conducted to identify the presence of Campylobacter jejuni in chicken meat by polymerase chain reaction. 36 samples were collected from different parts of Chennai and were tested for the presence of Campylobacter jejuni by targeting Hyp gene with 500bp by PCR. None of the samples were shown to be positive for Campylobacter jejuni.

4p nguaconbaynhay5 16-05-2020 7 0   Download

Salmonellosis is one of the most commonly reported food borne disease all over the world and developing countries. This study was conducted to determine the prevalence of Salmonella spp. in raw milk. Isolation and identification of Salmonella was carried out using real time polymerase chain reaction (RT-PCR) technique and sensitivity tests were done by the Kirby-Bauer disc diffusion method.

6p nguathienthan4 18-04-2020 18 3   Download

E. coli O157:H7 is an emerging food borne pathogen having zoonotic significance. Though the primary reservoir of this serotype is cattle; Sheep and goat are also considered to be the main reservoir for E. coli O157:H7. Conventional cultural methods are time consuming method and also the specificity is very less. Multiplex PCR (mPCR) tends to be specific, more rapid and reliable.

9p chauchaungayxua4 18-03-2020 18 1   Download

Pigeon pea (Cajanus cajan L. Millsp.) is one of the major grain legume crops of the tropics and sub-tropics grown in about 50 countries of Asia, Eastern and Southern Africa and the Caribbean for various uses such as food, fodder and firewood. The present study was conducted to develop and optimize tissue culture independent in planta transformation system in pigeonpea. This system can help in widening genetic base as well as solutions over various biotic and abiotic factors through engineering novel genes across the species. The various transformation parameters viz.

13p nguathienthan2 26-12-2019 11 0   Download

The presence of Escherichia coli (EC) microorganisms in live organs can cause foodborne illnesses and food-related deaths. Here, EC assay was performed using a microcopper three-electrode (MCE) system, where a handmade MCE was used as a working electrode and Ag/AgCl as reference and platinum counter electrodes. Under a 1.0-ml EC standard, the diagnostic optimum conditions were sought. The analytical oxidation potential was obtained at -0.2 V via positive scan. Under these conditions, the stripping linear working range was attained with 0.2-0.7 mg/mL EC variations.

10p nguathienthan2 26-12-2019 13 0   Download

The present study was undertaken to evaluate the status of ESBL producing Enterobacteriaceae in foods of animal origin and their environment. A total of 125 samples were collected comprising 95 animal products (40 raw milk, 25 milk products, 15 raw meat and 15 meat products) and 30 environmental samples. The isolation rate was recorded 93.95% in food samples with Citrobacter (38.41%) being the dominant flora, while100% in environmental samples with the dominance of E. coli (89.18%). Of all the ESBL producers, 24.29% were found positive by phenotypic method while 16.

10p nguathienthan2 26-12-2019 40 0   Download

Cassava (Manihot esculenta Crantz) is one of the most important crops in Vietnam for providing food, starch sources, and raw materials for the production of bio-ethanol and other purposes. Cassava bacterial blight (CBB) disease, caused by Xanthonomas axonopodis pv. manihotis (Xam), is one of the most important factors affecting cassava production in Vietnam. A rapid and sensitive molecular tool is required to support the traditional method, primarily based on biochemical reactions, that was considered less sensitive and time-consuming.

6p caygaocaolon1 13-11-2019 25 1   Download

Molecular species detection in food has become common in the last years. In this study, multiplex PCR (mPCR) technique was applied to discriminate between the pork and beef with the aim of detecting the pork in food products made from beef.

7p vititan2711 14-08-2019 13 0   Download