Peptidase combined enzymes

In the present investigation, sea rough fish were hydrolyzed with alcalase/peptidase combined enzymes (EA/EP). Raw material proximate composition was investigated. The results indicated that sea rough fish was successfully converted into hydrolysates that mainly composed of small size peptides and free amino acids. The essential amino acid could be served as a valuable source of nutrition for humans and animals.

10p viannee 02-08-2023 3 3   Download

In this study we investigate the active-site structure and the catalytic mechanism of clostripain by using a combination of three separate techniques: affinity labelling, site-directed mutagenesis and molecular modelling. A benzamidinyl-diazo dichlorotriazine dye (BDD) was shown to act as an efficient active site-directed affinity label for Clostridium histolyticumclostripain. The enzyme, upon incubation with BDD in 0.1MHepes/NaOHbuffer pH 7.6, exhibits a time-dependent loss of activity.

10p dell39 03-04-2013 51 2   Download