intTypePromotion=1
zunia.vn Tuyển sinh 2024 dành cho Gen-Z zunia.vn zunia.vn
ADSENSE

Báo cáo khóa học: The structure–function relationship in the clostripain family of peptidases

Chia sẻ: Nguyen Thang | Ngày: | Loại File: PDF | Số trang:10

54
lượt xem
3
download
 
  Download Vui lòng tải xuống để xem tài liệu đầy đủ

In this study we investigate the active-site structure and the catalytic mechanism of clostripain by using a combination of three separate techniques: affinity labelling, site-directed mutagenesis and molecular modelling. A benzamidinyl-diazo dichlorotriazine dye (BDD) was shown to act as an efficient active site-directed affinity label for Clostridium histolyticumclostripain. The enzyme, upon incubation with BDD in 0.1MHepes/NaOHbuffer pH 7.6, exhibits a time-dependent loss of activity.

Chủ đề:
Lưu

Nội dung Text: Báo cáo khóa học: The structure–function relationship in the clostripain family of peptidases

ADSENSE

CÓ THỂ BẠN MUỐN DOWNLOAD

 

Đồng bộ tài khoản
2=>2