Phanerochaete

Purified manganese peroxidase (MnP) fromPhanerocha-ete chrysosporiumoxidizes nonphenolicb-1 diarylpropane lignin model compounds in the presence of Tween 80,and in three- to fourfold lower yield in its absence. In thepresence of Tween 80,1-(3¢,4¢-diethoxyphenyl)-1-hydroxy-2-(4¢-methoxyphenyl)propane (I) was oxidized to 3,4-diethoxy-benzaldehyde (II),4-methoxyacetophenone (III) and 1-(3¢,4¢-diethoxyphenyl)-1-oxo-2-(4¢-methoxyphenyl)pro-pane (IV),while only 3,4-diethoxybenzaldehyde (II) and 4-methoxyacetophenone (III) were detected when the reac-tion was conducted in the absence of Tween 80..

9p tumor12 20-04-2013 41 4   Download

Cellobiose dehydrogenase (CDH) is an extracellular flavocytochrome con-taining flavin and b-type heme, and plays a key role in cellulose degrada-tion by filamentous fungi. To investigate intermolecular electron transfer from CDH to cytochrome c, Phe166, which is located in the cytochrome domain and approaches one of propionates of heme, was mutated to Tyr, and the thermodynamic and kinetic properties of the mutant (F166Y) were compared with those of the wild-type (WT) enzyme.

0p awards 06-04-2013 35 2   Download

The cellobiohydrolase Pc_Cel7D is the major cellulase produced by the white-rot fungusPhanerochaete chrysosporium, constituting
10% of the total secreted protein in liquid culture on cellulose. The enzyme is classified into family 7 of the glycoside hydrolases and, like other family members, catalyses cellulose hydrolysis with net retention of the anomeric carbon configuration.

0p awards 06-04-2013 29 2   Download

The basidiomycetePhanerochaete chrysosporiumproduces xyloglucanase Xgh74B, which has the glycoside hydrolase (GH) family 74 catalytic domain and family 1 carbohydrate-binding module, in cellulose-grown cul-ture.

10p media19 04-03-2013 26 2   Download

The 1,3(4)-b-d-glucanases of glycoside hydrolase family 16 provide useful examples of versatile yet specific protein–carbohydrate interactions. In the present study, we report the X-ray structures of the 1,3(4)-b-d-glucanase Phanerochaete chrysosporiumLaminarinase 16A in complex withb-glucan products from laminarin (1.6 A˚ ) and lichenin (1.1 A ˚ ) hydrolysis. The G6G3G3G glucan, in complex with the enzyme, showed ab-1,6 branch in the acceptor site.

12p vinaphone15 25-02-2013 41 2   Download

Tuyển tập các báo cáo nghiên cứu khoa học ngành toán học được đăng trên tạp chí toán học quấc tế đề tài: A calmodulin inhibitor, W-7 influences the effect of cyclic adenosine 3', 5'-monophosphate signaling on ligninolytic enzyme gene expression in Phanerochaete chrysosporium

34p dauphong11 04-02-2012 44 5   Download