Phosphate dehydrogenase

Sàng lọc, chẩn đoán sớm bệnh thiếu G6PD (Glucose-6-phosphate dehydrogenase) ở trẻ sơ sinh giúp phòng ngừa phát bệnh, đặc biệt là việc hướng dẫn chế độ dinh dưỡng chăm sóc bé cho gia đình. Bài viết trình bày xác định tỷ lệ thiếu men G6PD và tìm hiểu một số yếu tố liên quan ở trẻ sơ sinh.

6p viharuno 11-01-2025 2 0   Download

Bài viết trình bày kết luận: Chương trình sàng lọc sơ sinh tại Bệnh viện Phụ Sản Nhi - Đà Nẵng đã phát hiện được 1,12% trẻ có nguy cơ cao thiếu enzyme G6PD. Phần lớn trẻ được chẩn đoán xác định thuộc nhóm II và III, trẻ nam chiếm đa số, triệu chứng lâm sàng phổ biến là vàng da.

7p vibloomberg 31-12-2024 4 2   Download

- Polysaccharides are macromolecules, polymers with a few hundred to a few thousand monosaccharides joined by glycosidic linkages. - Some polysaccharides serve as storage material, hydrolyzed as needed to provide sugar for cells. - Other polysaccharides serve as building material for structures that protect the cell or the whole organism. - The architecture and function of a polysaccharide are determined by its sugar monomers and by the positions of its glycosidic linkages.

118p zingzing09 21-10-2012 54 8   Download

Two enzymes control the rearrangement of carbon skeletons which result in the production of Glyceraldehyde-3-phosphate and Fructose-6-phosphate. Transketolase transfers C2 units: TPP requiring enzyme like pyruvate dehydrogenase Transaldolase transfers C3 units: uses a shiffs base with an active lysine group

44p zingzing09 21-10-2012 53 11   Download

Bài giảng Thiếu men G6PD giúp bạn hiểu thêm về bệnh thiếu men G6PD, tần suất mắc bệnh thiếu G6PD, nguy cơ mắc bệnh, phòng ngừa hậu quả của thiếu men G6PD, các loại thuốc cần tránh dùng cho người bệnh thiếu G6PD,... Mời các bạn cùng tham khảo!

18p gongyuefei 10-08-2021 21 2   Download

Nội dung nghiên cứu của luận án là khảo sát ảnh hưởng của LXN và RMK đến các enzyme chuyển hóa carbohydrate gồm các enzyme là glucose-6-phosphatase (G6Pase), glucose-6- phosphate dehydrogenase (G6PDH) và lactate dehydrogenase (LDH) in vitro. Khảo sát khả năng chống stress oxy hóa và bảo vệ tế bào tụy tạng min6 khỏi sự chết do tunicamycin gây ra của LXN và RMK.

19p elysale 09-06-2021 15 2   Download

Đề tài nghiên cứu nhằm 2 mục tiêu: Xác định tỷ lệ nhiễm ký sinh trùng sốt rét tại một số điểm điều tra của tỉnh Đăk Nông năm 2017; xác định tỷ lệ thiếu G6PD của người dân sống trong vùng sốt rét lưu hành tại điểm điều tra.

70p capheviahe27 23-02-2021 22 4   Download

The involvement of the lysine residue present at the active site of Ehrlich ascites carcinoma (EAC) cell glyceraldehyde-3-phosphate dehydrogenase (Gra3P DH) was investigated by using the lysine specific reagents trinitrobenzenesulfonic acid (TNBS) and pyridoxal phosphate (PP). Both TNBS and PP inactivated EAC cell Gra3P DH with pseudo-first-order kinetics with the rate dependent on modifier concentration. Kinetic analysis, including a Tsou plot, indicated that both TNBS and PP apparently react with one lysine residue per enzyme molecule.

8p system191 01-06-2013 38 3   Download

We previously reported that GTS1 is involved in regulating ultradian oscillations of the glycolytic pathway induced by cyanide in cell suspensions as well as oscillations of energy metabolism in aerobic continuous cultures. Here, we screened a yeast cDNA library for proteins that bind to Gts1p using the yeast two-hybrid system and cloned multiple TDH cDNAs encoding the glycolytic enzyme glyceraldehyde-3-phosphate dehydrogenase (GAPDH).

10p research12 01-06-2013 41 3   Download

Cloning and over-expression of human glucose 6-phosphate dehydrogenase (Glc6P dehydrogenase) has for the first time allowed a detailed kinetic study of a preparation that is genetically homogeneous and in which all the protein molecules are of identical age. The steady-state kinetics of the recombinant enzyme, studied by fluorimetric initial-rate measurements, gave converging linear Lineweaver–Burk plots as expected for a ternary-complex mechanism.

8p research12 01-06-2013 64 4   Download

The ÔMediterraneanÕ variant of glucose-6-phosphate dehydrogenase (G6PD) deficiency is due to the C563CT point mutation, leading to replacement of Ser with Phe at position 188, resulting in acute haemolysis triggered by oxidants. Previous work has shown increased formation of altered aspartate residues in membrane proteins during cell ageing and in response to oxidative stress in normal erythrocytes. These abnormal residues are specifically recognized by the repair enzyme L-isoaspartate (D-aspartate) protein O-methyltransferase (PCMT; EC 2.1.1.77). ...

8p research12 01-06-2013 39 4   Download

A gene having high sequence homology (45–49%) with the glycerol-1-phosphate dehydrogenase gene from Methanobacterium thermoautotrophicum was cloned from the aerobic hyperthermophilic archaeon Aeropyrum pernix K1 (JCM 9820). This gene expressed in Escherichia coli with the pET vector system consists of 1113 nucleotides with an ATG initiation codon and a TAG termination codon. The molecular mass of the purified enzyme was estimated to be 38 kDa by SDS/PAGE and 72.4 kDa by gel column chromatography, indicating presence as a dimer....

8p research12 01-06-2013 27 4   Download

To gain better insight into the insulin secretory activity of fetalbcells in response to glucose, the expression of glucose transporter 2 (GLUT-2), glucokinase and mitochondrial glycerol phosphate dehydrogenase (mGDH) were studied. Expression ofGLUT-2mRNA and protein in pancreatic islets and liver was signi®cantly lower in fetal and suckling rats than in adult rats.

9p research12 29-04-2013 47 4   Download

Reactionof certainpeptides andproteinswithsingletoxygen (generated by visible light in the presence of rose bengal dye) yields long-lived peptide and protein peroxides. Incubation of these peroxides with glyceraldehyde-3-phosphate dehy-drogenase, in the absence of addedmetal ions, results in loss of enzymatic activity. Comparative studies with a range of peroxides have shown that this inhibition is concentration, peroxide, and time dependent, with H2O2less ecient than some peptide peroxides.

10p research12 23-04-2013 39 4   Download

Pycnoporus cinnabarinuslaccaselac1genewasoverexpressed in Aspergillus niger, a well-known fungal host producing a large amount of homologous or heterologous enzymes for industrial applications. The corresponding cDNA was placed under the control of the glyceraldehyde-3-phosphate dehydrogenase promoter as a strong and constitutive pro-moter. The laccase signal peptide or the glucoamylase preprosequence ofA.

8p research12 23-04-2013 52 5   Download

Light/dark modulation of the higher plant Calvin-cycle enzymes phosphoribulokinase (PRK) and NADP-depend-ent glyceraldehyde 3-phosphate dehydrogenase (NADP-GAPDH-A2B2) involves changes of their aggregation state inaddition to redox changes of regulatory cysteines.Herewe demonstrate that plants possess two different complexes containing the inactive forms (a) of NADP-GAPDH and PRK and (b) of only NADP-GAPDH, respectively, in darkened chloroplasts.

8p tumor12 22-04-2013 32 2   Download

A4 glyceraldehyde 3-phosphate dehydrogenase (GAPDH) was purified from the green algaChlamydomonas reinhardtii andwas alsooverexpressed inEscherichia coli. Bothpurified A4 tetramers of recombinant and native GAPDH were characterized for the first time. The pH optimum for both nativeandrecombinant enzymeswas close to7.8.ThepKsof theresidues involvedincatalysis indicate thatacysteineanda histidinemay take part in catalysis by chloroplast GAPDH, as is the case for glycolytic GAPDH.

8p tumor12 20-04-2013 37 3   Download

We report here the first crystal structure of a stable isosteric analogue of 1,3-bisphospho-D-glyceric acid (1,3-BPGA) bound to the catalytic domain of Trypanosoma cruzi glycosomal glyceraldehyde-3-phosphate dehydrogenase (gGAPDH)in which the two phosphoryl moieties interact with Arg249. This complex possibly illustrates a step of the catalytic process by which Arg249 may induce compression of the product formed, allowing its expulsion fromthe active site.

13p fptmusic 12-04-2013 70 3   Download

The synthesis of ATP in the human parasiteEntamoeba histolyticais car-ried out solely by the glycolytic pathway. Little kinetic and structural infor-mation is available for most of the pathway enzymes.

17p awards 06-04-2013 34 3   Download

Depolarization and repolarization phases (D and R phases, respectively) of mitochondrial potential fluctuations induced by photoactivation of the fluorescent probe tetramethylrhodamine methyl ester (TMRM) were ana-lyzed separately and investigated using specific inhibitors and substrates. The frequency of R phases was significantly inhibited by oligomycin and aurovertin (mitochondrial ATP synthase inhibitors), rotenone (mitochond-rial complex I inhibitor) and iodoacetic acid (inhibitor of the glycolytic enzyme glyceraldehyde-3-phosphate dehydrogenase)....

11p awards 06-04-2013 48 2   Download