Plant cells

FtsZ is conserved in almost all bacteria and is an essential protein for bacterial cell division. The cell division is prevented by the inhibitors against FtsZ’s functions. Thus, FtsZ is known as an attractive, and underexploited novel antibacterial target protein. The aim of this research is searching for new antibacterial agents from 72 plants collected in Central Vietnam.

6p viuchiha 03-01-2025 2 1   Download

An applied field of biology that involves the use of living organisms and bioprocesses in engineering, technology, medicine and other fields requiring bioproducts. (http://en.wikipedia.org/wiki/Biotechnology) It is the products and exploitation from other fundamental sciences.Traditional Biotechnology Traditional biotechnology refers to a number of ancient ways of using living organisms to make new products or modify existing ones. In its broadest definition, traditional biotechnology can be traced back to human's transition from hunter-gatherer to farmer.

29p zingzing09 24-04-2013 64 9   Download

Polymerase Chain Reaction is widely held as one of the most important inventions of the 20th century in molecular biology. Small amounts of the genetic material can now be amplified to be able to a identify, manipulate DNA, detect infectious organisms, including the viruses that cause AIDS, hepatitis, tuberculosis, detect genetic variations, including mutations, in human genes and numerous other tasks.

62p zingzing09 24-04-2013 56 6   Download

Glofish is the first transgenic animal approved to be consumed by human in the USA  The insertion of different constructs of GFP into the fish genomes to give different green colors.The idea for recombinant DNA was first proposed by Peter Lobban, a graduate student of Prof. Dale Kaiser in the Biochemistry Department at Stanford University Medical School. The first publications describing the successful production and intracellular replication of recombinant DNA appeared in 1972 and 1973.

54p zingzing09 24-04-2013 77 5   Download

Eucalyptus is one of the important plant species for paper pulp, fuel wood, and timber. In this study, a hybrid Eucalyptus, U16 line, was investigated for rapid in vitro propagation. The apical and adventitious shoots served as initial explants.

8p viprimi 16-12-2024 1 1   Download

The objective of the study was to find the explant resources, the type and concentration of plant growth regulators (PGRs), as well as the in vitro culture conditions suitable for different morphogenesis processes - callus induction, direct embryogenesis, shoot and root formation, etc. The growth and development of in vitro Ngoc Linh ginseng derived from thin cell layer (TCL) was examined in Quang Nam to assess the adaptability of in vitro Ngoc Linh plantlets in its natural territory, compared to those growing in Bidoup - Nui Ba National Park, Lam Dong.

27p capheviahe27 23-02-2021 21 5   Download

The signaling properties of the gasotransmitter molecule hydrogen sulfide (H2S), which is endogenously generated in plant cells, are mainly observed during persulfidation, a protein post-translational modification (PTM) that affects redox-sensitive cysteine residues. There is growing experimental evidence that H2S in higher plants may function as a mechanism of response to environmental stress conditions.

7p partimesinhvien 08-05-2020 31 0   Download

The objective of the study was to find the explant resources, the type and concentration of plant growth regulators (PGRs), as well as the in vitro culture conditions suitable for different morphogenesis processes - callus induction, direct embryogenesis, shoot and root formation, etc. The growth and development of in vitro Ngoc Linh ginseng derived from thin cell layer (TCL) was examined in Quang Nam to assess the adaptability of in vitro Ngoc Linh plantlets in its natural territory, compared to those growing in Bidoup - Nui Ba National Park, Lam Dong.

27p xacxuoc4321 09-07-2019 35 5   Download

Business Unit Cell Cybernetics, Plant Research International, Wageningen, the Netherlands; 2University of Wu¨rzburg, Chair of Food Chemistry, Germany; 3Laboratory of Plant Physiology, Wageningen University, the Netherlands Citrus limon possesses a high content and large variety of monoterpenoids, especially in the glands of the fruit flavedo. The genes responsible for the production of these monoterpenes have never been isolated.

12p system191 01-06-2013 42 7   Download

Laboratory for Phytopathology and Plant Protection, Katholieke Universiteit Leuven, Belgium; 2Institut de Pharmacologie et Biologie Structurale, UMR-CNRS 5089, Toulouse Cedex, France; 3Rega Institute, Laboratory of Molecular Immunology, Katholieke Universiteit Leuven, Belgium Fruits of elderberry (Sambucus nigra) express small quantities of a type-2 ribosome-inactivating protein with an exclusive specificity towards the NeuAc(a2,6)Gal/GalNAc disaccharide and a unique molecular structure typified by the occurrence of a disulfide bridge between the B-chains of two adjacent protomers....

10p system191 01-06-2013 43 3   Download

Agrobacterium tumefaciens is a Gram-negative, phytopathogenic bacterium and is characterized by an unique mode of action on dicotyledonous plants: it is able to genetically modify the host, and because of this feature, it is used as a tool for transgenic plants. Many experiments have demonstrated that lipopolysaccharides (LPSs) play an important role for the disease development, as they are involved in the adhesion process of the bacterium on the plant cell wall.

4p system191 01-06-2013 32 4   Download

Manganese lipoxygenase was isolated to homogeneity from the take-all fungus, Gaeumannomyces graminis. The C-terminal amino acids and several internal peptides were sequenced, and the information was used to obtain a cDNA probe by RT/PCR. Screening of a genomic library of G. graminis yielded a full-length clone of the Mn-Lipoxygenase gene. cDNA analysis showed that the gene spanned 2.6 kb and contained one intron (133 bp). Northern blot analyses indicated two transcripts (2.7 and 3.1 kb). The deduced amino-acid sequence of the Mn-Lipoxygenase precursor (618 amino acids, 67.

8p system191 01-06-2013 36 4   Download

Biozentrum-Pharmazie, Universitat Halle, Halle/Saale, Germany; 2Lehrstuhl fur Organische Chemie und Biochemie, Technische ¨ ¨ ¨ ¨ ¨ Universitat Munchen, Garching, Germany; 3Laboratorium fur Organische Chemie, Eidgenossische Technische Hochschule Honggerberg, ¨ Zurich, Switzerland 2C-Methyl-D -erythritol 2,4-cyclodiphosphate was recently shown to be formed from 2C-methyl-D -erythritol 4-phosphate by the consecutive action of IspD, IspE, and IspF proteins in the nonmevalonate pathway of terpenoid biosynthesis. ...

9p system191 01-06-2013 41 4   Download

Expansins are a family of proteins that catalyze pHdependent long-term extension of isolated plant cell walls. They are divided into two groups, a and b, the latter consisting of the grass group I pollen allergens and their vegetative homologs. Expansins are suggested to mediate plant cell growth by interfering with either structural proteins or the polysaccharide network in the cell wall.

10p research12 01-06-2013 30 4   Download

The Na + /H + exchanger is a ubiquitous membrane protein of bacteria, plants and mammals. The first isoform discov-ered (NHE1) is present on the mammalian plasma mem-brane and transports oneH + out of cells inexchange for one extracellular Na + . With solubilization in standard SDS/ PAGE buffer, this protein had a high tendency to aggregate when subjected to elevated temperature. The aggregates were stable and did not dissociate in high concentrations of SDSor 2-mercaptoethanol.

9p research12 29-04-2013 46 3   Download

We are interested in constructing a model for the substrate-bindingsiteof fattyacidelongase-13-ketoacylCoAsynthase (FAE1 KCS),the enzyme responsible forproductionof very long chain fatty acids of plant seed oils.Arabidopsis thaliana andBrassica napusFAE1 KCS enzymes are highly homo-logous but the seed oil content of these plants suggests that their substrate specificities differ with respect to acyl chain length.

10p research12 29-04-2013 31 3   Download

The photosynthetic protein complexes in plants are located in the chloroplast thylakoid membranes. These membranes have an ultrastructure that consists of tightly stackedÔgranaÕ regions interconnectedbyunstackedmembrane regions.The structure of isolated granamembranes has been studiedhere by cryo-electron microscopy. The data reveals an unusual arrangement of the photosynthetic protein complexes, staggered over two tightly stacked planes.

11p research12 29-04-2013 42 2   Download

important regulators of biological processes in animals and plants. MiRNAs regulate gene expression at the posttranscriptional level by binding to mRNAs and either inhibit translation or modify the stability of the mRNA. Due to the important biological role of miRNAs it is of great interest to study their expression level in the cells. Furthermore, miRNAs have been associated with cancer and other diseases [1] and miRNA expression can help in the diagnosis and prognostic of human disease [2,3].

11p zingzing09 24-04-2013 52 3   Download

The present study reports the first purification and kinetic characterization of two plant arogenate dehydrogenases (EC 1.3.1.43), an enzyme that catalyses the oxidative decarboxylation of arogenate into tyrosine in presence of NADP. The twoArabidopsis thalianaarogenate dehy-drogenases TyrAAT1 and TyrAAT2 were overproduced in Escherichia coliand purified to homogeneity. Bio-chemical comparison of the two forms revealed that at low substrate concentration TyrAAT1 is four times more efficient in catalyzing the arogenate dehydrogenase reac-tion than TyrAAT2. ...

9p research12 23-04-2013 48 4   Download

Plant cell wall proteins called expansins are thought to disrupt hydrogen bonding between cell wall polysaccha-rides without hydrolyzing them. We describe here a novel gene with sequence similarity to plant expansins, isolated from the cellulolytic fungus Trichoderma reesei. The pro-tein named swollenin has an N-terminal fungal type cel-lulose binding domain connected by a linker region to the expansin-like domain.

10p research12 23-04-2013 36 4   Download