Polymorphism

Kỹ thuật RFLP là kỹ thuật nghiên cứu tính đa hình chiều dài của các phân đoạn DNA dựa trên điểm cắt các enzim giới hạn. Khi ủ DNA với enzim giới hạn ở dung dịch đệm thích hợp ở pH, nhiệt độ thích hợp sẽ sẽ tạo ra những phân đoạn DNA với kích thước khác nhau, từ đó lập nên các bản đồ gen. Tham khảo chương hai "Kỹ thuật RFLP - Restriction fragment length polymorphisms" để hiểu hơn về vấn đề này.

10p thanhbinh2305 09-11-2015 257 64   Download

As Vietnam has rich resources of local chicken breeds, some of them are known having a certain level of infectious disease resistance. Knowledge of the single-nucleotide polymorphism in exon 14 of the chicken Mx gene will facilitate further researches on the resistant activity of Mx gene and its use for breeding of influenza virus resistant chicken varieties. This study aimed to investigate the exon 14 polymorphisms of the Mx gene in some representative indigenous chicken breeds collected from different locals in Vietnam.

6p nganga_08 12-10-2015 18 2   Download

Certain phenomena resist coverage within the Lambek Calculus, such as scopeambiguity and non-peripheral extraction. I have argued in previous work that an extension called Polymorphic Lambek Calculus (PLC), which adds variables and their universal quantification, covers these phenomena. However, a major problem is the absence of a known decision procedure for PLC grammars. This paper proposes a decision procedure which covers a subset of all the possible PLC grammars, a subset which, however, includes the PLC grammars with wide coverage. ...

10p buncha_1 08-05-2013 25 1   Download

Object-Oriented Programming: Polymorphism..1..Outline 10.1   Introduction 10.2   Relationships Among Objects in an Inheritance Hierarchy 10.2.1  Invoking Base­Class Functions from Derived­Class  Objects 10.2.2 

92p sakuraphuong 04-06-2013 47 1   Download

Introduction to java programming: Chapter 9 - Inheritance and Polymorphism's Objectives is to develop a subclass from a superclass through inheritance; invoke the superclass’s constructors and methods using the super keyword; override methods in the subclass; distinguish differences between overriding and overloading.

56p cocacola_17 09-12-2015 22 1   Download

In this chapter, you will learn: The concept of polymorphism, to use overridden methods to effect polymorphism, to distinguish between abstract and concrete classes, to declare abstract methods to create abstract classes, how polymorphism makes systems extensible and maintainable, to determine an object's type at execution time, to declare and implement interfaces.

115p nhanmotchut_3 19-10-2016 17 1   Download

Object Oriented Programming - Lesson 8: Polymorphism and generic programming Master the polymorphism technique, Understand the Java generic programming, Polymorphism, Downcasting and upcasting, Overloading, Method call binding, Generic programming.

46p maiyeumaiyeu26 04-01-2017 21 1   Download

In this study, we designed two PCR primers to amplify the region containing rs6548238. The AvaI restriction enzyme was selected to detect the SNP polymorphism. Sequencing the resulting bands validated the RFLP method. In conclusion, RFLP method is simple, sensitive and cost effective for genotyping of the TMEM18 rs6548238 polymorphism; thus, this method is most convenient for SNP analysis in large-size samples from Vietnamese population.

7p jangni2 19-04-2018 9 0   Download

The influence of intensity Taylor vortex flow in Couette-Taylor crystallizer on the crystallization of polymorphic amino acid Lglutamic acid was investigated in cooling crystallization. Here, the L-glutamic acid was chosen as the model crystal product, where it has two kinds of polymorphism including the unstable phase α-form and stable phase β-form crystal.

13p ironman1234 06-06-2018 4 0   Download

This study aimed to investigate the effects of cytochrome P450 1A1 (CYP1A1) gene polymorphism on early ageing due to occupational exposure to mutagens. The project was conducted on buccal epithelial cells collected from mechanical-workshop workers and controls.

7p sony2711 01-02-2019 4 0   Download

Random amplified polymorphic DNA (RAPD) markers were successfully used in discrimination of sexes in Nile tilapia fish (Oreochromis niloticus) using linear discriminant function analysis. The results provide support for the view that major genetical sex determining factors exist in tilapia.

7p caplock2711 19-02-2019 8 0   Download

Due to advances in molecular biology techniques, large numbers of highly informative DNA markers have been developed for the identification of genetic polymorphism. In the last decade, the random amplified polymorphic DNA (RAPD) technique based on the polymerase chain reaction (PCR) has been one of the most commonly used molecular techniques to develop DNA markers.

12p caplock2711 19-02-2019 10 0   Download

Compared to other Polymerase Chain Reaction (PCR) based molecular markers, the Amplified Fragment Length Polymorphism (AFLP) marker system probably requires more technical expertise for the sequential enzymatic reaction steps.

9p caplock2711 19-02-2019 5 0   Download

To separate the unknown Streptomyces strains isolated from soil samples, the interspacer regions of 16S-23S rDNA of 14 isolates were amplified with PCR (polymerase chain reaction) and digested with three restriction endonucleases, namely, Bsp143I, HaeIII and MnlI. The restriction patterns were used for RFLP (restriction fragment length polymorphism) analysis.

8p caplock2711 19-02-2019 9 0   Download

Simple sequence repeat (SSR) DNA polymorphism is a PCR-based marker system, also known as the microsatellite marker system. SSRs are tandemly repeated 1 to 5 nucleotide units dispersed throughout eukaryotic genomes.

4p caplock2711 19-02-2019 4 0   Download

The frequency of the ‘‘A’’ allele of the Gly972Arg (G>A) polymorphism was similar between the normal glucose and prediabetic subjects (2.7% and 2.6%, respectively). There was no significant difference in the genotypic frequency between the control and prediabetic cases (P = 0.673). The IRS1 Gly972Arg polymorphism was not associated with the risk of prediabetes in Vietnamese women both before and after being adjusted for socio-economic, lifestyle and clinical factors (P > 0.05).

7p cathydoll4 21-02-2019 4 0   Download

Breast cancer is the most common malignant tumor in women and accounts for about 25% of all cancer diagnoses. Survivin is a member of the apoptosis inhibitor protein family of antiapoptotic proteins. In our study, we investigated one of those, the survivin gene promoter 31G/C polymorphism.

9p vikimsa 22-02-2019 7 0   Download

Differentially responding genotypes are comparatively used to understand mechanisms of metal tolerance in higher plants. The feasibility of the random amplified polymorphic DNA (RAPD) technique was evaluated to screen for metal tolerance within plants as an alternative to physiological growth parameters.

10p vikimsa 22-02-2019 6 0   Download

In this chapter you will learn: The concept of polymorphism, to use overridden methods to effect polymorphism, to distinguish between abstract and concrete classes, to declare abstract methods to create abstract classes, how polymorphism makes systems extensible and maintainable, to determine an object's type at execution time. to declare and implement interfaces.

90p shiwo_ding8 29-06-2019 4 1   Download

To establish and complete a protocol for genotyping of single polymorphism rs165599 on catechol-O-methyltransferase (COMT) gene by PCR-RFLP. Methods: DNA samples of rs165599 known genotypes by sequencing, using techniques including PCR amplification, restriction fragment length polymorphism, and agarose gel electrophoresis to determine parameters for optimization of the protocol.

6p visamurai2711 25-07-2019 0 0   Download