Powdered Crinum latifolium L

Phenolics are a class of chemical compounds consisting of a hydroxyl group bonded directly to an aromatic hydrocarbon group. There are two groups of phenolics - simple phenols and polyphenols. Polyphenols whose antioxidant activity have the ability to reduce or prevent damage caused by free radicals promote positive effects on human health [5]. Therefore, in this present study, we tried to determine the total phenolic content of powdered Crinum latifolium L. bulbs extract.

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The purpose of study is extraction and isolation of hippadine from the bulbs of Crinum latifolium for using as a reference standard for quality control of herbs and hippadine-containing preparations. Bulbs powder of Crinum latifolium was percolated with 70% ethanol, percolate obtained was evaporated at 65 0C to give a liquid extract. The extract was fractionated by distribution with n-hexane. The n-hexane extract was then chromatographed on silicagel 60 (0.04 - 0.063 mm). Hippadine was isolated, purified and elucidated by NMR, MS, IR and UV-Vis spectrum.

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