Pre-implantation
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This study aimed to establish culture medium changing system to improve the efficiency of bovine somatic cell nuclear transfer protocol. Our results showed that using the culture medium of Takahashi and First 1992 produced the cloned bovine blastocyst at the highest rate (9.4%, P < 0.05) among different modified synthetic oviduct fluid (mSOF) media.
4p visergey 02-04-2024 4 0 Download
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Very low-coverage (0.1 to 1×) whole genome sequencing (WGS) has become a promising and aford‑ able approach to discover genomic variants of human populations for genome-wide association study (GWAS).
18p vicwell 29-02-2024 2 1 Download
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The high incidence of aneuploidy in early human development, arising either from errors in meiosis or postzygotic mitosis, is the primary cause of pregnancy loss, miscarriage, and stillbirth following natural conception as well as in vitro fertilization (IVF).
15p vicwell 29-02-2024 9 2 Download
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To evaluate the correlation between the concentration of AMH (Anti-mullerian hormone) with the number of oocytes and embryos obtained in general and in each maternal age group, in particular on infertile patients undergoing in vitro fertilization (IVF) combined with preimplantation genetic testing for aneuploidy (PGT-A).
9p videadpool 06-05-2023 3 2 Download
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This study aimed to examine the effect of HDACi treatment during the ZGA period. In this study, the effect of HDACi treatment with different concentrations on preimplantation development of cloned bovine embryos is done.
7p vimalfoy 08-02-2023 6 1 Download
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This study examined the effects of the cumulus cell co-culture system on the development of cloned bovine embryos. The results showed that the coculture of cloned bovine embryos with cumulus cells significantly improved the in vitro development rate to the blastocyst stage (25 %) compared to the control group (12.5 %).
8p vimalfoy 08-02-2023 6 2 Download
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In conclusion, activation by cycloheximide was the most qualified chemical for artificial activation and preimplantation development of the Bos gaurus-taurus interspecies embryo. However, H3K9me3 immunostaining can only detect the Bos gaurus pronucleus formation at the zygote stage.
8p vimalfoy 08-02-2023 5 1 Download
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Production of cloned bovine has many practical applications in medicine and agriculture, although low efficiency has still been the main problem. This challenge has resulted from improper epigenetic reprogramming of donor's nuclear such as histone acetylation and zygotic gene activation that does not occur correctly.
8p vimalfoy 08-02-2023 3 1 Download
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In this research, we evaluated the preimplantation development, embryo morphology, the time of cell division and the cell number of the blastocyst stage of single mouse blastomeres biopsied from 8-cell embryos. One blastomere was biopsied from each 8-cell embryo using micromanipulation system with XYClone laser.
8p vimalfoy 08-02-2023 1 1 Download
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Animal cloning is the process of producing an entire organism that theoretically has the same genome as its donor cell line. To validate the success of SCNT, one must verify that the genetic source of the cloned organism is identical to the donor cell. However, none of the studies have performed to identify the genetic characteristics in the stage of preimplantation development of cloned embryo.
8p vimalfoy 08-02-2023 1 1 Download
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This study examined the effects of Scriptaid, a histone deacetylase inhibitor (HDAC) during the first mitotic cell cycle on preimplantation development of mouse embryos generated by injection of round spermatid into mature oocytes.
8p vimalfoy 08-02-2023 4 1 Download
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Bài viết Phân tích di truyền trước làm tổ bệnh Hemophilia B báo cáo một trường hợp gia đình thực hiện chẩn đoán di truyền trước làm tổ (Preimplantation genetic testing for monogenic disorders, PGT-M) bệnh Hemophilia B (HEMB) sàng lọc thành công phôi không mang bệnh.
7p vihermione 23-12-2022 24 2 Download
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Bài viết trình bày khảo sát sự phân bố bất thường kiểu trisomy và monosomy của phôi ở ba nhóm bệnh nhân: bệnh nhân lớn tuổi (advanced maternal age – AMA), sẩy thai liên tiếp (recurrent miscarriage - RM) và thất bại làm tổ nhiều lần (Repeated Implantation Failure - RIF) điều trị thụ tinh trong ống nghiệm (TTTON) kết hợp sàng lọc lệch bội ở phôi (Preimplantation Genectic Testing for Aneuploidies – PGT-A).
8p viedison 13-04-2022 32 2 Download
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Thalassemia là bệnh lý thiếu máu tán huyết di truyền đơn gen. Kỹ thuật chẩn đoán đột biến di truyền đơn gen giai đoạn phôi tiền làm tổ (Preimplantation Genetic Testing for Monogenic disorders - PGT-M) là kỹ thuật phân tích di truyền phôi nhằm giảm nguy cơ mắc bệnh di truyền trên thai nhi ở các cặp vợ chồng mang gen bệnh. Bài viết trình bày đánh giá hiệu quả có thai khi thực hiện chuyển phôi được thực hiện PGT-M để loại các phôi mang đột biến gen gây bệnh thalassemia.
8p viedison 13-04-2022 23 2 Download
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Hiện tượng lệch bội nhiễm sắc thể ở phôi là một trong những nguyên nhân chính gây thất bại làm tổ, sẩy thai liên tiếp, làm giảm hiệu quả điều trị thụ tinh trong ống nghiệm. Xét nghiệm di truyền phôi tiền làm tổ phát hiện phôi lệch bội (Preimplantation Genetic Testing for Aneuploidy) giúp sàng lọc, phát hiện các phôi mang bất thường về số lượng nhiễm sắc thể bằng cách thu nhận 5-10 tế bào lá nuôi phôi (Trophectoderm).
5p vilouispasteur 10-03-2022 31 4 Download
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Pigs are important animals for agricultural and biomedical research, and improvement is needed for use of the assisted reproductive technologies. Determining underlying mechanisms of epigenetic reprogramming in the early stage of preimplantation embryos derived from in vitro fertilization (IVF), parthenogenesis, and androgenesis will not only contribute to assisted reproductive technologies of pigs but also will shed light into early human development.
21p viarchimedes 26-01-2022 48 0 Download
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This technique is commercially expanding due to many improvements in embryo technology. In this study, we attempted to reduce the embryonic invasiveness and develop a simple, fast, and reliable sexing procedure using direct amplification of Y chromosome-specific marker from only one blastomere.
7p spiritedaway36 28-11-2021 11 0 Download
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Aberrant DNA methylation patterns of genes required for development are common in in vitro produced embryos. In this regard, we previously identified altered DNA methylation patterns of in vivo developed blastocysts from embryos which spent different stages of development in vitro, indicating carryover effects of suboptimal culture conditions on epigenetic signatures of preimplantation embryos.
19p vibeauty 23-10-2021 6 1 Download
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Embryonic and fetal exposure to maternal obesity causes several maladaptive morphological and epigenetic changes in exposed offspring. The timing of these events is unclear, but changes can be observed even after a short exposure to maternal obesity around the time of conception. The hypothesis of this work is that maternal obesity influences the ovine preimplantation conceptus early in pregnancy, and this exposure will affect gene expression in embryonic and extraembryonic tissues.
12p vitzuyu2711 29-09-2021 11 1 Download
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While DNA and RNA methods are routine to disrupt the expression of specific genes, complete understanding of developmental processes requires also protein methods, because: oocytes and early embryos accumulate proteins and these are not directly affected by DNA and RNA methods.
19p visilicon2711 20-08-2021 11 1 Download