Proteomic strategy

Ebook "Modern molecular biology: Approaches for Unbiased discovery in cancer research" is to provide an overview of modern molecular biological approaches to unbiased discovery in cancer research. Advances in molecular biology allowing unbiased analysis of changes in cancer initiation and progression will be overviewed. These include the strategies employed in modern genomics, gene expression analysis, and proteomics.

196p cotieubac1004 15-03-2024 1 0   Download

Ebook "Sample preparation in biological mass spectrometry" is to provide the researcher with important sample preparation strategies in a wide variety of analyte molecules, specimens, methods, and biological applications requiring mass spectrometric analysis as a detection end-point. In this volume we have compiled the contributions from several laboratories which are employing mass spectrometry for biological analysis.

1081p manmanthanhla0201 26-02-2024 2 2   Download

Ebook "Geomicrobiology: Molecular and environmental perspective" covers the interaction of microorganisms with geological activities resulting in processes influencing development of the Earth's geo- and biospheres. Addresses environmental proteomics, functional gene arrays, isotope labelling, bioinformatics & related techniques to analyze relationships & functions of microbes in complex, natural communities.

445p tachieuhoa 28-01-2024 3 2   Download

Ebook "Activity-based proteomics: Methods and protocols" focuses on explorative activity-based proteomics, biomedical applications of activity-based proteomics, and chemical strategies in activity-based proteomics providing a concise overview of activity-based protein profiling. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

222p lucchinguyen 28-12-2023 18 2   Download

Ebook "Investigations of early nutrition effects on long-term health: Methods and applications" contains several reviews in the field as well as the step-by-step use of targeted and global approaches within the areas of genomics, epigenetics, proteomics, transcriptomics and metabolomics which aim to address this dilemma and to help pinpoint new treatment strategies. Chapters detail the generation of several models and methods for assessing health and provide researchers potential approaches for reversing or minimizing effects of disease.

512p lucchinguyen 28-12-2023 4 2   Download

Ebook "Peptidomics: Methods and strategies" describes protocols for basic state-of-the-art approaches in the field of peptidomics. Most of these approaches are independent of the instruments used for analysis and can easily be adapted for equipment that is available in a typical proteomics facility. Chapters detail many of the basic techniques used to detect and identify peptides, methods for the relative quantitation of peptides between samples using isotopic labels or label-free approaches, and biological species as well as sample types.

423p lucchinguyen 28-12-2023 8 2   Download

Part 1 book "Farm animal proteomics" includes content: Mass spectrometry for the veterinary and farm animal world, protein synthesis pipelines for study of proteinprotein interactions, dinosaurs and other farm animals, proteomics strategies to trace illegal growthpromoters in cattle, assessing fish quality in aquaculture - a proteomics approach,.... and other contents

113p muasambanhan02 18-12-2023 12 0   Download

Part 1 book "Farm animal proteomics 2013" includes content: Mining deeper into the proteome - pros and cons of pre-fractionation and depletion; detection and annotation of common posttranslational modifications in mass spectrometry data, applied bioinformatics in the structural, postgenomic era, PTMomics – a potpourri of experimental approaches; gel-free quantitative proteomics approaches, current status; new proteomics strategies applied to clinical studies,... and other contents.

168p muasambanhan02 18-12-2023 0 0   Download

Part 2 book "Farm animal proteomics 2014" includes content: Using shotgun proteomics to understand the effect of feed restriction on the Ovis aries wool proteome, comparative proteomic analysis of muscle tissue from pre-term and term calves, high frequencies of the αS1-casein zero variant and its relation to coagulation properties in milk from Swedish dairy goats,... and other contents.

70p muasambanhan02 18-12-2023 7 2   Download

Part 1 book "Biochemistry" includes content: Biochemistry - an evolving science, protein composition and structure, exploring proteins and proteomes, DNA, RNA and the flow of genetic information, exploring genes and genomes, exploring evolution and bioinformatics, hemoglobin - portrait of a protein in action, enzymes - basic concepts and kinetics, catalytic strategies, regulatory strategies, carbohydrates, lipids and cell membranes, membrane channels and pumps, signal transduction pathways.

455p oursky07 24-10-2023 10 4   Download

Sheep are valuable resources for the animal fibre industry. Therefore, identifying genes which regulate wool growth would offer strategies for improving the quality of fine wool. In this study, we employed Agilent sheep gene expression microarray and proteomic technology to compare the gene expression patterns of the body side (hair-rich) and groin (hairless) skins of Aohan fine wool sheep (a Chinese indigenous breed).

11p vinarcissa 21-03-2023 1 1   Download

A proteomic workflow for a simple loss-less manual nano-fractionation (300 nL/fraction) for low μg sample amounts which avoids the need to dry down or transfer fractions to autosampler vials is shown to be feasible. It is demonstrated that the conventional procedure of drying samples down followed by reconstitution negatively affects the number of protein and peptide identifications.

10p viginny 23-12-2022 8 2   Download

We present a scalable, integrated strategy for coupled protein and RNA detection from single cells. Our approach leverages the DNA polymerase activity of reverse transcriptase to simultaneously perform proximity extension assays and complementary DNA synthesis in the same reaction.

15p viaristotle 29-01-2022 6 0   Download

Coexistence and transition of diverse sex determination strategies have been revealed in some ectothermic species, but the variation between males caused by different sex determination strategies and the underlying mechanism remain unclear. Here, we used the gynogenetic gibel carp (Carassius gibelio) with both genotypic sex determination (GSD) and temperature-dependent sex determination (TSD) strategies to illustrate this issue.

15p vibeauty 23-10-2021 14 1   Download

Bacillus cereus is a notorious foodborne pathogen, which can grow under anoxic conditions. Anoxic growth is supported by endogenous redox metabolism, for which the thiol redox proteome serves as an interface. Here, we studied the cysteine (Cys) proteome dynamics of B. cereus ATCC 14579 cells grown under fermentative anoxic conditions. We used a quantitative thiol trapping method combined with proteomics profiling.

15p vitzuyu2711 29-09-2021 7 0   Download

Nitraria tangutorum is an important desert shrub that shows resistance to drought, salt and wind erosion stresses. It is a central ecological species in its area. Here, we have studied how N. tangutorum has adapted to achieve a successful reproduction strategy.

13p vijichea2711 28-05-2021 11 1   Download

Present review aims to accumulate animal oocyte findings in terms of protein research in oocyte through available literature, which remain to be emphasized for oocyte proteomics.

6p chauchaungayxua10 19-03-2021 7 2   Download

Protein complexes carry out nearly all signaling and functional processes within cells. The study of protein complexes is an effective strategy to analyze cellular functions and biological processes. With the increasing availability of proteomics data, various computational methods have recently been developed to predict protein complexes.

14p vioklahoma2711 19-11-2020 7 2   Download

This work presents a machine learning strategy to increase sensitivity in tandem mass spectrometry (MS/MS) data analysis for peptide/protein identification. MS/MS yields thousands of spectra in a single run which are then interpreted by software.

14p vioklahoma2711 19-11-2020 9 1   Download

Utilization of quantitative proteomics data on the network level is still a challenge in proteomics data analysis. Currently existing models use sophisticated, sometimes hard to implement analysis techniques. Our aim was to generate a relatively simple strategy for quantitative proteomics data analysis in order to utilize as much of the data generated in a proteomics experiment as possible.

18p vicolorado2711 23-10-2020 16 1   Download