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Quantitative polymerase chain reaction
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We present our current study, which investigated the prevalence of harbouring BCR::ABL1 in the normal first-degree relatives of local CML patients (designated as StudyR ). We compared and discussed the prevalence of StudyR and StudyN to assess the familial influence in CML occurrence.
11p
vishanshan
27-06-2024
3
1
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This study was designed to investigate the regulatory effects of kinesin family member (KIF) 23 on anaplastic thyroid cancer (ATC) cell viability and migration and the underlying mechanism.
10p
vikoch
27-06-2024
1
1
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Cervical cancer (CC) is a common gynecological malignancy worldwide. Some patients perform serious resistance after chemotherapy, and long-stranded non-coding RNA MEG3 is reported to be involved in the regulation of chemoresistance in many solid tumors. However, its involvement in cervical adenocarcinoma has not been reported.
13p
vileonardodavinci
23-12-2023
4
3
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Part 2 book "RT-PCR Protocols" includes content: Changes in gene expression of caveolin-1-after inflammatory pain using quantitative real time PCR; real time quantitative reverse transcriptase polymerase chain reaction; hot start PCR; primer design for RT-PCR,... and other contens.
150p
oursky05
20-09-2023
1
0
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RNASET2 has been identified as an oncogene with anti-angiogenic and immunomodulatory effects in a variety of cancers, but its function in clear cell renal cell carcinoma (ccRCC) is still not well understood.
16p
vioracle
29-09-2023
5
2
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Part 2 book "Clinical applications pf PCR" includes content: Quantitative real time PCR - Recent advances, single cell quantitative PCR - advances and potential in cancer diagnostics, detection of trypanosoma cruzi by polymerase chain reaction, polymerase chain reaction diagnosis of leishmaniasis - a species specific approach,... and other contents.
104p
oursky05
20-09-2023
3
1
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Hu sheep, a unique Chinese breed with high reproductive performance, are also well known for their rare white lambskin in China. The quality of lambskin is affected by hair follicles, and dermal papilla cells are an important component of hair follicles that plays a key role in hair follicle growth and development.
12p
vihagrid
30-01-2023
6
3
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Reportedly, circular RNA (circRNA) is a key modulator in the development of human malignancies. This work is aimed to probe the expression pattern, biological effects and mechanism of circ_0064288 on hepatocellular carcinoma (HCC) progression.
11p
vikolindagrabar
27-07-2022
3
1
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Sự điều hòa quá trình sinh trưởng và biệt hóa tinh trùng ở nam giới do nhiều gen quy định. Gen SPAG8 (Sperm associated antigen 8) là một trong những gen tham gia vào quá trình đó mà vai trò của nó còn chưa được minh chứng rõ ràng. Bài viết trình bày việc hoàn thiện quy trình kỹ thuật RT-qPCR (Revese transcription - quantitative polymerase chain reaction) để đánh giá mức độ biểu hiện mRNA của gen SPAG8 ở nam giới vô sinh.
5p
vimarillynhewson
17-05-2022
20
1
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Results showed that the Microcystis genus dominated the cyanobacterial communities in the TAR. Microcystis was also the primary microcystins (MC) producing cyanobacteria in the water. Total cyanobacteria and Microcystis cells ranged from 152×103 to 27×106 copy/L and from 105×103 to 19×106 copy/L, respectively. The cell number of potentially MC-producing cyanobacteria (corresponding to the Microcystis mcyDgene) varied from 27×103 to 13×106 copy/L.
8p
spiritedaway36
28-11-2021
11
0
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This study aimed to explore the role and underlying molecular mechanisms of long non-coding RNA (lncRNA) LINC00342 in gastric cancer (GC). The expression of LINC00342 in GC tissues was evaluated by Quantitative reverse transcription polymerase chain reaction (qRT-PCR). Silencing of LINC00342 was conducted to investigate the effect of LINC00342 in vitro and in vivo.
12p
vimahuateng
26-11-2021
4
1
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This case-control study was conducted on 67 patients with PCa and 40 control subjects. The expression levels of cellular and viral factors involved in inflammation, tumor progression, and metastasis were quantified, using the enzyme-linked immunosorbent assay (ELISA) and quantitative real-time polymerase chain reaction (qRTPCR) assay.
19p
vimahuateng
26-11-2021
6
1
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PIKfyve is an evolutionarily conserved lipid and protein kinase enzyme that has pleiotropic cellular functions. The aim of the present study was to investigate the effects of phosphatidylinositol-3-phosphate 5-kinase (PIKfyve) inhibitor, YM201636, on nonsmall cell lung cancer (NSCLC) cells growth, tumorigenicity, and claudin (CLDN) expressions. Three NSCLC cell lines (Calu-1, H1299 and HCC827) were used to compare the effects of YM201636. Cytotoxic effects of YM201636 were analysed using XTT assay.
9p
thiencuuchu
27-11-2021
10
1
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GTN stimulation increased both CRLR/ CGRPR1 expression, and immunostaining was apparent in microglia and meningeal cells. This study showed for the first time that GTN modulates intracellular iron trafficking regulated by storage and transport proteins expressed in meningeal cells and glia. CRLR/ CGRPR1 expression might be related to altered iron homeostasis and they both may stimulate nociceptive pathways activated in migraine.
10p
thiencuuchu
27-11-2021
6
0
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Pyropia yezoensis, a marine red alga, is an ideal research model for studying the mechanisms of abiotic stress tolerance in intertidal seaweed. Real-time quantitative polymerase chain reaction (RT-qPCR) is the most commonly used method to analyze gene expression levels. To accurately quantify gene expression, selection and validation of stable reference genes is required.
14p
vibeauty
23-10-2021
7
0
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The consensus on how to choose a reference gene for serum or plasma miRNA expression qPCR studies has not been reached and none of the potential candidates have yet been convincingly validated. We proposed a new in silico approach of finding a suitable reference for human, circulating miRNAs and identified a new set of endogenous reference miRNA based on miRNA profiling experiments from Gene Expression Omnibus.
15p
vijeeni2711
24-07-2021
7
0
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In quantitative real-time polymerase chain reaction (qRT-PCR) experiments, accurate and reliable target gene expression results are dependent on optimal amplification of house-keeping genes (HKGs). RNA-seq technology offers a novel approach to detect new HKGs with improved stability.
16p
vijeeni2711
24-07-2021
5
0
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Numerous models for use in interpreting quantitative PCR (qPCR) data are present in recent literature. The most commonly used models assume the amplification in qPCR is exponential and fit an exponential model with a constant rate of increase to a select part of the curve.
13p
viwyoming2711
16-12-2020
9
0
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Quantitative molecular methods (QMMs) such as quantitative real-time polymerase chain reaction (q-PCR), reverse-transcriptase PCR (qRT-PCR) and quantitative nucleic acid sequence-based amplification (QT-NASBA) are increasingly used to estimate pathogen density in a variety of clinical and epidemiological contexts.
11p
vikentucky2711
26-11-2020
8
1
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Accurate adjustment for the amplification efficiency (AE) is an important part of real-time quantitative polymerase chain reaction (qPCR) experiments. The most commonly used correction strategy is to estimate the AE by dilution experiments and use this as a plug-in when efficiency correcting the Cq.
14p
vioklahoma2711
19-11-2020
7
1
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