Real time expression

We aimed to screen novel gene signatures for ovarian cancer (OC) and explore the role of biomarkers in OC via regulating pyroptosis using bioinformatics analysis. The proliferation, migration, and invasion were determined using cell counting kit and transwell assay. Enzyme-linked immunosorbent assay was applied to measure infammatory factors. The mRNA and protein expression were detected using real-time quantitative PCR and western blot.

15p vishanshan 27-06-2024 1 1   Download

In this study, all the expressions of Otubain-1, NF-κB2, SHPs and STATs genes, the concentrations of cytokines IL-1β, IL-6 and TNF-α and clinical features in NHL patients were examined. To the end, gene expression levels of 82 NHL patients and 56 healthy individuals were determined by quantitative real time RT-PCR and secretion of cytokines by ELISA.

10p dianmotminh02 03-05-2024 5 1   Download

The deregulation of microRNA-140 (miRNA-140) has been reported to be involved in the human pathogenesis of Osteoarthritis (OA). However, in Vietnam, to our knowledge, there is a limited study of evaluation of a miRNA-140 expression. The commercial kits for the quantitation of miRNA-140 are more expensive.

7p vibego 02-02-2024 2 0   Download

Ebook "Potassium channels: Methods and protocols" provides readers with the latest techniques and approaches used to study K+ channels. There are a wide range of topics covered in the book, such as channel structure; K+ channels and their roles in physiology and disease; single-molecule FRET; proteins expression levels in vivo; real-time correlation of channel activity; gating; pharmacology; physiological functions; and channelopathies.

346p lucchinguyen 28-12-2023 3 2   Download

Cell division cycle 6 (CDC6) is a key licensing factor in the assembly of pre-replicative complexes at origins of replication. The role of CDC6 in the pathogenesis of in diffuse larger B-cell lymphoma (DLBCL) remains unknown. We aim to investigate the effects of CDC6 on the proliferation, apoptosis and cell cycle regulation in DLBCL cells, delineate its underlying mechanism, and to correlate CDC6 expression with clinical characteristics and prognosis of patients with DLBCL.

11p visharma 20-10-2023 5 2   Download

Part 2 book "PCR Technology - Current innovations" includes content: Assay design for Real-Time qPCR, primer design for large scale multiplex PCR and arrayed primer extension, hypothesis driven approaches to multivariate analysis of qPCR data, Experiment Design, data management, and univariate statistical analysis of gene expression data obtained by real time quantitative PCR,.... and other contents.

261p oursky06 17-10-2023 3 1   Download

Part 2 book "RT-PCR Protocols" includes content: Changes in gene expression of caveolin-1-after inflammatory pain using quantitative real time PCR; real time quantitative reverse transcriptase polymerase chain reaction; hot start PCR; primer design for RT-PCR,... and other contens.

150p oursky05 20-09-2023 2 0   Download

A long fruit (lf) mutant was obtained from the cucumber inbred line ‘SN5’ by ethyl methanesulfonate (EMS)-mediated mutagenesis. We found that plant height and internode length of lf were significantly decreased, while leaf area was significantly increased in comparison to wild type (WT) plants. Higher carbohydrate level, together with lower chlorophyll content and photosynthetic parameters, was revealed in lf leaves than those of WT.

13p lyhuyenthu 31-01-2023 8 2   Download

Fat mass and obesity-associated protein (FTO) is a demethylase and plays a vital role in various cancers. However, the regulation mechanism of FTO in prostate cancer (PCa) remains unclear. This study aimed to elucidate the mechanism of FTO in PCa. The function and mechanism of FTO-mediated in PCa were determined by gain-of-function assays and RNA-seq. We found that FTO expression in PCa tissues and two PCa cell lines were significantly lower than that in adjacent tissues and normal cell line.

14p lyhuyenthu 31-01-2023 7 2   Download

This study aims to study PASD1 protein as a potential target for CRC immunotherapy. A total of 90 CRC and polyps tissue samples were investigated for PASD1 RNA and protein expression using a real-time polymerase chain reaction and immunohistochemical staining, respectively. Matched patients’ peripheral blood mononuclear cells were pulsed with PASD1 peptides and measured for immunogenicity, cell cytotoxicity, and cytokine assays.

17p lyhuyenthu 31-01-2023 6 2   Download

Abnormal expression of long non-coding RNAs (lncRNAs) has been shown to be associated with the pathogenesis of cancers, including colorectal cancer (CRC). It has been reported that LINC00022 is highly expressed in some typs of cancer and its overexpression indicates poor prognosis.

15p vikolindagrabar 27-07-2022 3 1   Download

Reportedly, circular RNA (circRNA) is a key modulator in the development of human malignancies. This work is aimed to probe the expression pattern, biological effects and mechanism of circ_0064288 on hepatocellular carcinoma (HCC) progression.

11p vikolindagrabar 27-07-2022 3 1   Download

Real time RT-PCR (qPCR) is a useful and powerful tool for quantitative measurement of gene expression. The proper choice of internal standards such as reference genes is crucial for correct data evaluation. In female dogs, as in other species, the reproductive tract is continuously undergoing hormonal and cycle stagedependent morphological changes, which are associated with altered gene expression.

12p vigandhi 23-02-2022 9 1   Download

The expressions of Prunus dulcis C-repeat-binding factors (PdCBF1) and (PdCBF2) genes that are the major components in the cold responsive network of plants were studied in almonds. Real time PCR analysis of buds revealed the differential expression pattern of PpFT, PabSOC1, PdCBF1, PdCBF2 genes. The expressions of PpFT and PabSOC1 correlated with each other. Similarly, the expressions of PdCBF1 and PdCBF2 genes revealed a similar expression pattern in almonds. However, the expression of flowering genes were inversely correlated with the cold response genes in most of the almond accessions.

11p hanthienngao 30-11-2021 5 1   Download

The cDNA encoding lipase enzyme was cloned from Olea europaea var. europaea cv. Gemlik leaves using the RACE method. The full nucleotide sequence was 1546 bp long and encoded a putative protein of 412 amino acid long protein. The amino acid sequence showed identity to Triacylglycerol Lipase 1 sequences from different plants. A phylogenetic tree was constructed showing relations with various plant lipases.

8p tudichquannguyet 29-11-2021 11 1   Download

The rol oncogenes of Agrobacterium rhizogenes enhance the production of medicinally important compounds in plants and provide a first barrier against the overproduction of reactive oxygen species during biotic and abiotic stress. This study was designed to evaluate the expression of genes involved in biosynthetic pathways and their impact on metabolic contents and environmental stress tolerance in regenerated Ajuga bracteosa Wall. ex Benth. After successful transformation, real-time quantitative PCR confirmed the increased expression (1.94–6.

19p tudichquannguyet 29-11-2021 13 1   Download

Saintpaulia ionantha H. Wendl (synonym Streptocarpus ionanthus) has been considered an ornamental plant. The PHANTASTICA (PHAN) gene has a significant role in the formation of plant organs adaxial–abaxial polarity. In the present study the PHAN gene was identified in Saintpaulia for the first time using PCR. To determine the expression pattern of the PHAN gene, gene expression was compared at 3 developmental stages using real time PCR.

14p tudichquannguyet 29-11-2021 3 0   Download

This study was conducted to investigate the physicochemical, phytochemical, in vitro antidiabetic and anticancer potential of Olea ferruginea R bark. After extraction using Soxhlet, in vitro antidiabetic and cytotoxic activity on human hepatocellular carcinoma (HepG2) cells was assessed by nonenzymatic glycosylation of hemoglobin assay, alpha-amylase inhibition assay, glucose uptake by yeast cells, and 3-[4,5-dimethylthiazol-2-yl]-2,5 diphenyl tetrazolium bromide assay, respectively, and gene expression via real-time polymerase chain reaction.

12p tudichquannguyet 29-11-2021 12 0   Download

The present study aimed to synthesize ursolic acid-loaded chitosan nanoparticles (UA-Ch-NPs) as an antiinfective agent against 21 Staphylococcus aureus isolates. The UA-Ch-NPs were synthesized by a simple method and then characterized by TEM, FTIR, DLS-zeta potential, and XRD analyses. According to the characterization results, highly dispersed spherical nanoparticles with a mean diameter of 258 nm and a zeta potential of + 40.1 mV were developed. The antibacterial properties of UA-Ch-NPs were investigated and their inhibitory effect on biofilm formation was demonstrated by AFM.

9p tudichquannguyet 29-11-2021 9 2   Download

This case-control study was conducted on 67 patients with PCa and 40 control subjects. The expression levels of cellular and viral factors involved in inflammation, tumor progression, and metastasis were quantified, using the enzyme-linked immunosorbent assay (ELISA) and quantitative real-time polymerase chain reaction (qRTPCR) assay.

19p vimahuateng 26-11-2021 7 1   Download