Report experiment

Those living with disadvantage suffer from higher rates of chronic conditions and multimorbidities. Little work has been done to investigate how this cohort experience patient-centred care and what their outcomes might be. This study showed that as a result of a short course of treatment clients experienced better levels of health and wellbeing and decreased levels of pain and disability. Interview data suggest that this may be due to a combination of therapy, setting and relationships formed within that setting.

177p runthenight04 02-02-2023 5 2   Download

This study primarily concentrates on the metaphors used in economic reports. Its aim is to have an insight into how metaphors are correlated to human physical experience and constructed in economic reports. In addition, the analysis of this correlation will set foundation for later categorization of the source domains which are exploited in the metaphors in these economic reports.

87p closefriend09 16-11-2021 25 4   Download

In the first lesson of inorganic chemistry lab, preparing and testing solutions are two basic tasks needed to perform well. Dissolving a solid solute in the solvent or diluting the stock solution (a dilution series by using two techniques that are making parallel dilutions and serial dilutions) is to prepare solution with desired concentration of solute or desired volume.

11p suplo28475858 24-05-2020 37 3   Download

Direct and oversee an organization's sales policies, objectives and initiatives. Set short- and long-term sales strategies and evaluate effectiveness of current sales programs. Recommend product or service enhancements to improve customer satisfaction and sales potential. Familiar with a variety of the field's concepts, practices and procedures. Rely on extensive experience and judgment to plan and accomplish goals. Lead and direct the work of others. A wide degree of creativity and latitude is expected. Typically reports to top management.

3p lanjingyi 18-03-2020 61 4   Download

The design of b-glycosidases with planed substrate specificity for biotechnological application has received little attention. This is mostly a consequence of the lack of data on the molecular basis of the b-glycosidase specificity, namely data on the energy of the noncovalent interactions in the enzymetransition state complex. In an attempt to fill this gap, sitedirected mutagenesis and enzyme steady-state kinetic experiments with different substrates were conducted, using as model a digestive b-glycosidase (glycoside hydrolase family 1) from Spodoptera frugiperda (Lepidoptera) (Sfbgly50).

10p system191 01-06-2013 45 4   Download

2-Aminoethoxydiphenyl Borate (2-APB) has been extensively used recently as a membrane permeable modulator of inositol-1,4,5-trisphosphate-sensitive Ca2+ channels and store-operated Ca2+ entry. Here, we report that 2-APB is also an inhibitor of sarco/endoplasmic reticulum Ca2+ATPase (SERCA) Ca2+ pumps, and additionally increases ion leakage across the phospholipid bilayer. Therefore, we advise caution in the interpretation of results when used in Ca2+ signalling experiments.

10p system191 01-06-2013 41 4   Download

Pyruvate decarboxylase (EC 4.1.1.1) was isolated and purified from the yeast Kluyveromyces lactis. The properties of this enzyme relating to the native oligomeric state, the subunit size, the nucleotide sequence of the coding gene(s), the catalytic activity, and protein fluorescence as well as circular dichroism are very similar to those of the well characterized pyruvate decarboxylase species from yeast.

8p system191 01-06-2013 44 3   Download

The case of homologous monomeric c-type and oligomeric b-type crystallins has been described and analyzed in evolutionary terms. Data and hypotheses from molecular genetics and structural investigations converge and suggest a novel three-phase model for the evolutionary history of crystallin-type proteins. In the divergent cascades of monomeric and oligomeric crystallins, a pivotal role was played by alterations in the gene segments encoding the C-terminal extensions and the intermotif or interdomain linker peptides.

9p system191 01-06-2013 43 4   Download

Modelling of the fluxes in central metabolism can be performed by combining labelling experiments with metabolite balancing. Using this approach, multiple samples from a cultivation of Saccharomyces cerevisiae in metabolic and isotopic steady state were analysed, and the metabolic fluxes in central metabolism were estimated. In the various samples, the estimates of the central metabolic pathways, the tricarboxylic acid cycle, the oxidative pentose phosphate pathway and the anaplerotic pathway, showed an unprecedented reproducibility....

6p system191 01-06-2013 43 2   Download

Agrobacterium tumefaciens is a Gram-negative, phytopathogenic bacterium and is characterized by an unique mode of action on dicotyledonous plants: it is able to genetically modify the host, and because of this feature, it is used as a tool for transgenic plants. Many experiments have demonstrated that lipopolysaccharides (LPSs) play an important role for the disease development, as they are involved in the adhesion process of the bacterium on the plant cell wall.

4p system191 01-06-2013 33 4   Download

Mitochondrial functional and structural impairment and generation of oxidative stress have been implicated in aging, various diseases and chemotherapies. This study analyzed azidothymidine (AZT)-caused failures in mitochondrial functions, in redox regulation and activation of the HIV-1 gene expression. We monitored intracellular concentrations of ATP and glutathione (GSH) as the indicators of energy production and redox conditions, respectively, during the time-course experiments with U937 and MOLT4 human lymphoid cells in the presence of AZT (0.05 mgÆmL)1) or H2O2 (0.01 mM) for 15–25 days.

7p system191 01-06-2013 43 3   Download

Frog p26olf is a novel S100-like Ca21-binding protein found in olfactory cilia. It consists of two S100-like domains aligned sequentially, and has a total of four Ca21-binding sites (known as EF-hands). In this study, to elucidate the mechanism of Ca21-binding to each EF-hand (named EF-A, -B, -C and -D from the N-terminus of p26olf), we examined Ca21-binding in wild-type p26olf and also in its mutants in which a glutamate at the –z coordinate position within each Ca21-binding loop was substituted for a glutamine.

8p system191 01-06-2013 29 4   Download

The function of squalene-hopene cyclase from Alicyclobacillus acidocaldarius was studied by labelling critical cysteine residues of the enzyme, either native or inserted by sitedirected mutagenesis, with different thiol-reacting molecules. The access of the substrate to the active centre cavity through a nonpolar channel that contains a narrow constriction harbouring a cysteine residue (C435) was probed by labelling experiments on both a C435S mutant, lacking C435 of the channel constriction, and a C25S/C50S/C455S/C537S mutant, bearing C435 as the only cysteine residue.

9p research12 01-06-2013 46 3   Download

The human replication protein Cdc6p is translocated from its chromatin sites to the cytoplasm during the replication phase (S phase) of the cell cycle. However, the amounts of Cdc6p on chromatin remain high during S phase implying either that displaced Cdc6p can rebind to chromatin, or that Cdc6p is synthesized de novo. We have performed metabolic labeling experiments and determined that [35S]methionine is incorporated into Cdc6p at similar rates during the G1 phase and the S phase of the cell cycle. Newly synthesized Cdc6p associates with chromatin....

7p research12 01-06-2013 41 3   Download

To identify and gain a better understanding of the cadherin-like receptor-binding site onBacillus thuringiensisCry toxins, it is advantageous to use Cry1Aa toxin, because its 3D structure is known. Therefore, Cry1Aa toxin was used to examine the locations of cadherin-like protein-binding sites. Initial experiments examining the binding compatibility for Cry1Aa toxin of partial fragments of recombinant proteins of a 175 kDa cadherin-like protein from Bombyx mori(BtR175) and another putative receptor for Cry1Aa toxin, aminopeptidase N1, fromBo. ...

14p research12 29-04-2013 45 3   Download

Although a steadily increasing number of protein±ligand docking experiments have been performed successfully, there are only few studies concerning protein±sugar inter-actions. In this study, we investigate the interaction of wheat germ agglutinin (WGA) withN-acetylglucosamine and a number of its derivatives and predict the binding free ener-gies using ¯exible docking techniques.

7p research12 29-04-2013 36 2   Download

TheO-chainpolysaccharide of the lipopolysaccharide (LPS) of a previously nonclassi®ed strain ofProteus mirabilis termed G1 was studied by sugar analysis and 1 Hand13 C NMR spectroscopy, including 2D COSY, TOCSY, rota-ting-frameNOE(ROESY),H-detected 1 H, 13 CHMQC,and heteronuclear multiple-bond correlation (HMBC) experi-ments.

7p research12 29-04-2013 49 5   Download

Human aromatase is responsible for estrogen biosynthesis and is implicated, in particular, in reproduction and estro-gen-dependent tumor proliferation. Themolecular structure model is largelyderived fromtheX-ray structure of bacterial cytochromes sharing only 15±20% identities with hP-450arom. In the present study, site directed mutagenesis experiments were performed to examine the role of K119, C124, I125, K130, E302, F320, D309, H475, D476, S470, I471 and I474 of aromatase in catalysis and for substrate binding....

13p research12 29-04-2013 34 3   Download

Ikkai&Ooi [Ikkai,T.&Ooi,T. (1966)Biochemistry5, 1551± 1560] made a thorough study of the e€ect of pressure on G- and F-actins. However, all of the measurements in their study were made after the release of pressure. In the present experiment in situ observations were attempted by using eATP to obtain further detailed kinetic and thermodynamic information about the behaviour of actin under pressure.

8p research12 29-04-2013 41 7   Download

Mild acid degradation of the lipopolysaccharide ofCitro-bacter gilleniiO9a,9b released a polysaccharide (PS), which was found to consist of a single monosaccharide, 4-acetamido-4,6-dideoxy-D-mannose (D-Rha4NAc,N-acetyl-D-perosamine). PS was studied by methylation analysis and 1 H-NMR and 13 C-NMR spectroscopy, using two-dimen-sional 1 H, 1 H COSY, TOCSY, NOESY, and H-detected 1 H, 13 C heteronuclear correlation experiments.

7p research12 29-04-2013 34 4   Download