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Restriction enzymes
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Ebook "Plant physiology" examines three ways plants respond to their changing environment. The first example can be found in all plants. Despite the extreme changes in weather, plants have to stay where they are and respond to whatever nature produces. Plants have the capacity to respond quickly and yet they can evolve in a single generation. The second example addresses how an individual leaf has to respond rapidly and repeatedly to maintain the proper balance of carbon dioxide (CO2) and water so that it can photosynthesize but not dry out.
57p
manmanthanhla0201
26-02-2024
6
1
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The impact of Fe2+ (iron)toxicity on genomic instability, DNA methylation status, and Long Terminal Repeat Retrotransposons (LTR RTs) polymorphisms on Zea mays is unknown. We investigated the toxicity of Fe2+ using Random Amplified Polymorphic DNA (RAPD), Coupled Restriction Enzyme Digestion-Random Amplification (CRED-RA) and Inter Retrotransposon Amplified Polymorphism (IRAP) assays in Zea mays seedlings, respectively.
8p
lyhuyenthu
31-01-2023
6
2
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Brewing is a highly complex stepwise process that starts with a mashing step during which starch is gelatinized and converted into oligo and/or monosaccharides by enzymes and heat. Previous studies have mostly been restricted to analysing the grain and/or malt prior to entering the brewing process, but here we track the fates of polysaccharides during the entire brewing process.
9p
vihermione
06-01-2023
10
2
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The Anatolian water buffalo is a native water buffalo breed only being reared in Turkey. The objective of this study was to investigate gene polymorphisms on exons 4 and 5 of the growth hormone (GH) and on exon 10 of the growth hormone receptor (GHR), which are thought to be related to meat yields of Anatolian water buffalo. Blood samples of 192 water buffaloes from Sivas Province were used in DNA extractions by phenol-chloroform method. DNA samples were amplified by using specific primers in PCR.
6p
dolomite36
30-12-2021
9
0
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The purpose of this study was to develop a fit-for-purpose methylation sensitive restriction enzyme (MSRE) based digital droplet PCR (ddPCR) assay to examine MLH1 promoter methylation in ctDNA in advanced CRC.
10p
vimahuateng
26-11-2021
10
3
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This study reports a new Helicoverpa armigera nucleopolyhedrovirus (NPV) isolated from Heliothis peltigera (Denis & Schiffermuller), collected in the vicinity of Adana, Turkey. Infection was confirmed by tissue polymerase chain reaction and sequence analysis. Results showed that dead H. peltigera larvae contain Helicoverpa armigera nucleopolyhedrovirus. Thus, the isolate was named as HearNPV-TR. Microscopy studies indicated that occlusion bodies were 0.73 to 1.66 μm in diameter. The nucleocapsids are approximately 184 × 41 nm in size.
9p
thiencuuchu
27-11-2021
7
1
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Bài giảng Sinh học phân tử: Chương 7 Kỹ thuật tạo dòng, cung cấp cho người học những kiến thức như: Các công cụ sử dụng trong kỹ thuật tạo dòng; Vai trò sinh học của RE; Tạo dòng phân tử (molecular cloning); Sự khác nhau của các vector tạo dòng; Plasmid là các vector tạo dòng;...Mời các bạn cùng tham khảo!
60p
caphesuadathemmatong
25-11-2021
23
1
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Restriction site-associated DNA sequencing (RADseq) has revolutionized the study of wild organisms by allowing cost-effective genotyping of thousands of loci. However, for species lacking reference genomes, it can be challenging to select the restriction enzyme that offers the best balance between the number of obtained RAD loci and depth of coverage, which is crucial for a successful outcome.
12p
vigiselle2711
30-08-2021
17
1
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Chapter 20 - DNA technology and genomics. This chapter presents the following content: DNA technology makes it possible to clone genes for basic research and commercial applications, restriction enzymes are used to make recombinant DNA, genes can be clones in recombinant DNA vectors,...
113p
tradaviahe19
29-03-2021
9
1
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Massive parallel sequencing is a powerful tool for variant discovery and genotyping. To reduce costs, sequencing of restriction enzyme based reduced representation libraries can be utilized. This technology is generally referred to as Genotyping By Sequencing (GBS). To deal with GBS experimental design and initial processing specific bioinformatic tools are needed.
6p
vikentucky2711
24-11-2020
14
1
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Physical mapping of DNA with restriction enzymes allows for the characterization and assembly of much longer molecules than is feasible with sequencing. However, assemblies of physical map data are sensitive to input parameters, which describe noise inherent in the data collection process.
6p
vioklahoma2711
19-11-2020
15
1
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Restriction site analysis involves determining the locations of restriction sites after the process of digestion by reconstructing their positions based on the lengths of the cut DNA. Using different reaction times with a single enzyme to cut DNA is a technique known as a partial digestion.
10p
vioklahoma2711
19-11-2020
9
0
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GmrSD is a modification-dependent restriction endonuclease that specifically targets and cleaves glucosylated hydroxymethylcytosine (glc-HMC) modified DNA. It is encoded either as two separate single-domain GmrS and GmrD proteins or as a single protein carrying both domains. Previous studies suggested that GmrS acts as endonuclease and NTPase whereas GmrD binds DNA.
16p
vioklahoma2711
19-11-2020
9
0
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Reference genome assemblies are valuable, as they provide insights into gene content, genetic evolution and domestication. The higher the quality of a reference genome assembly the more accurate the downstream analysis will be.
9p
viflorida2711
30-10-2020
13
2
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Genotyping-by-sequencing (GBS), a method to identify genetic variants and quickly genotype samples, reduces genome complexity by using restriction enzymes to divide the genome into fragments whose ends are sequenced on short-read sequencing platforms.
12p
viconnecticut2711
29-10-2020
9
2
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Chromatin 3D conformation plays important roles in regulating gene or protein functions. High-throughout chromosome conformation capture (3C)-based technologies, such as Hi-C, have been exploited to acquire the contact frequencies among genomic loci at genome-scale.
17p
vicolorado2711
22-10-2020
11
1
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n reduced representation sequencing, representative portion of genome is sequenced. Modified reduced representation sequencing method, called double digest Restriction Associated DNA sequencing (ddRADseq) uses two restriction enzymes for skipping the random shearing step of RAD Sequencing. Gradually ddRADseq acquired important role in high density SNP discovery and genotyping, in model and non-model organisms with high efficiency and low cost.
7p
caygaocaolon7
18-09-2020
12
0
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Tối ưu quy trình phân tích đa hình đơn nucleotid RS320 thuộc gen Lipoprotein lipase ở người Việt Nam
Để phân tích đa hình này ở quần thể người Việt Nam, nghiên cứu có mục tiêu tối ưu phương pháp khuếch đại gen - Đa hình chiều dài đoạn cắt giới hạn (Polymerase chain reaction-restriction fragment length polymorphism - PCR - RFLP) với các cặp mồi, chu trình nhiệt, enzyme giới hạn được lựa chọn, thử nghiệm để xác định điều kiện tối ưu.
7p
viamman2711
07-08-2020
17
1
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The aim of this study was to identify the Complex Vertebral Malformation (CVM) in repeat breeder and anoestrous animals in Maharashtra state region. Total 102 animals of HF crossbred, Dangi, Gaolao and Non- descript were screened from field and cattle dairy farm. CVM is a hereditary recessive disorder in Holstein cattle which have a significant economic impact on dairy cattle breeding worldwide. Fifty repeat breeder and fifty-two anoestrous animals blood DNA were extracted by traditional method. Genotyping was performed by using PCR-RFLP analysis with the help of PstI restriction enzyme.
4p
cothumenhmong5
17-05-2020
12
1
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Wheat production is largely restricted by adverse environmental stresses. Under many undesirable conditions, endoplasmic reticulum (ER) stress can be induced. However, the physiological and molecular responses of wheat to ER stress remain poorly understood.
22p
vishikamaru2711
25-04-2020
2
0
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