RNA interference

1976, Walter Fiers và cộng sự → trình tự hoàn chỉnh một RNA virus 1977, Richard Roberts và Phillip Sharp → alternative splicing 1986, Thomas R. Cech → self-splicing Kary Mullis → kỹ thuật PCR 1989, Thomas R. Cech và Sydney Altman → ribozyme 1990s, các cơ chế RNA antisense và RNA interference

27p htc_12 13-05-2013 112 32   Download

Đồ án tốt nghiệp này được thực hiện với mục tiêu nhằm nghiên cứu công nghệ bất hoạt RNA thông qua quy trình tổng hợp double stranded RNA theo bộ Kit của hãng Promega và Ambion; ứng dụng kỹ thuật bất hoạt RNA để tạo ra con cái giả phục vụ cho công tác tạo tôm càng xanh toàn đực với Mr–IAG là gene mục tiêu. Mời các bạn cùng tham khảo.

64p zhangyan 13-07-2021 35 6   Download

Head and neck squamous cell carcinoma (HNSCC) is the most common and most aggressive type of head and neck cancer. Current approaches for the treatment of HNSCC are not sufficient to increase the patient survival or to reduce the high recurrence rate.

12p nutifooddau 27-01-2019 25 2   Download

Antisense agents are valuable tools to inhibit the expression of a target gene in a sequence-specific manner,and may be used for functional genomics,target validation and thera-peuticpurposes.Three typesof anti-mRNAstrategies canbe distinguished. Firstly,the use of single stranded antisense-oligonucleotides; secondly,the triggering of RNA cleavage through catalytically active oligonucleotides referred to as ribozymes; and thirdly,RNA interference induced by small interfering RNA molecules.

17p fptmusic 16-04-2013 41 2   Download

During vitellogenesis, one of the most tightly regulated processes in ovipar-ous reproduction, vitellogenins are incorporated into the oocyte through vitellogenin receptor (VgR)-mediated endocytosis. In this paper, we report the cloning of the VgR cDNA from Blattella germanica, as well as the first functional analysis of VgR following an RNA interference (RNAi) approach.

11p dell39 27-03-2013 40 1   Download

The crustacean hyperglycemic hormone (CHH) plays an important role in the regulation of hemolymph glucose levels, but it is also involved in other functions such as growth, molting and reproduction. In the present study we describe the first CHH family gene isolated from the Atlantic Ocean shrimp Litopenaeus schmitti.

9p inspiron33 23-03-2013 43 4   Download

RNA interference (RNAi) mediates sequence-specific post-transcriptional gene silencing in many eukaryotes and is used for reverse genetic studies and therapeutics. RNAi is triggered by double-stranded small interfering RNAs (siRNAs), which can be processed from small hairpin RNAs gener-ated from an expression vector.

7p inspiron33 23-03-2013 37 2   Download

We developed a new method for inhibiting tobacco mosaic virus infection in tobacco plants based on specific RNA hydrolysis induced by a leadzyme. We identified a leadzyme substrate target sequence in genomic tobacco mosaic virus RNA and designed a 16-mer oligoribonucleotide capable of forming a specific leadzyme motif with a five-nucleotide catalytic loop.

10p inspiron33 23-03-2013 38 2   Download

RNA-mediated gene silencing (RNA interference) is a powerful way to knock down gene expression and has revolutionized the fields of cellular and molecular biology. Indeed, the transfection of cultured cells with small interfering RNAs (siRNAs) is currently considered to be the best and easi-est approach to loss-of-function experiments.

16p media19 06-03-2013 48 5   Download

Co 2+ binding RNA aptamers were chosen as research models to reveal the structural basis underlying the recognition of Co 2+ by RNA, with the application of two distinct methods. Using the nucleotide analog interfer-ence mapping assay, we found strong interference effects after incorpora-tion of the 7-deaza guanosine phosphorotioate analog into the RNA chain at equivalent positions G27 and G28 in aptamer no.

12p media19 06-03-2013 28 3   Download

One of the important peptide hormones that control reproduction in crus-taceans is gonad-inhibiting hormone (GIH). GIH is known to modulate gonad maturation by inhibiting synthesis of vitellogenin (Vg), the precursor of yolk proteins. In this study, a cDNA encoding a GIH (Pem-GIH) from the eyestalk of Penaeus monodonwas cloned using RT-PCR and RACE techniques.

11p media19 06-03-2013 23 4   Download

Although the crustacean crustacean hyperglycemic hormone⁄molt-inhibi-ting hormone⁄gonad-inhibiting hormone neuropeptides have been studied extensively in the last two decades and several neuropeptides from the shrimpMetapenaeus ensishave been cloned, the functions of most of these neuropeptides remained putative.

11p media19 04-03-2013 42 2   Download

African trypanosomes possess high levels of alanine aminotransferase (EC 2.6.1.2), although the function of their activity remains enigmatic, espe-cially in slender bloodstream forms where the metabolism of ketoacids does not occur. Therefore, the gene for alanine aminotransferase enzyme inTry-panosoma brucei(TbAAT) was characterized and its function assessed using a combination of RNA interference and gene knockout approaches.

13p vinaphone15 27-02-2013 55 3   Download

We report here a new method for inhibition of RNA viruses induced by dsDNA. We demonstrated that both long dsDNA molecules and short interfering DNA with a sequence complementary to that of viral RNA inhibited tobacco mosaic virus expression and prevented virus spread. Also, the expression of the HIV-1 gp41 gene in HeLa cells was inhibited by com-plementary short interfering DNA.

9p vinaphone15 25-02-2013 35 4   Download

Oocyte membrane localization of vitellogenin receptor coincides with queen flying age, and receptor silencing by RNAi disrupts egg formation in fire ant virgin queens Hsiao-Ling Lu, S. B. Vinson and Patricia V. Pietrantonio Department of Entomology, Texas A&M University, College Station, TX, USA Keywords insect ovary; insect reproduction; oocyte development; RNA interference; social insects Correspondence P. V. Pietrantonio, Department of Entomology, Texas A&M University, College Station, TX 77843-2475, USA Fax: +1 979 845 6305 Tel: +1 979 845 9728 E-mail: p-pietrantonio@tamu.

14p viettel02 22-02-2013 47 2   Download

This study investigated the potential of RNA interference, which is techni-cally challenging in bivalve mollusc species, to assess gene function in the oysterCrassostrea gigas. We designed dsRNA targeting the oyster vasa-like gene (Oyvlg), specifically expressed in oyster germ cells. In vivoinjection of oyvl-dsRNA into the gonad provokes a knockdown phenotype correspond-ing to germ cell underproliferation and prematurely arrested meiosis throu-gout the organ.

8p viettel02 22-02-2013 32 2   Download

Tuyển tập báo cáo các nghiên cứu khoa học quốc tế ngành hóa học dành cho các bạn yêu hóa học tham khảo đề tài: Inhibiting adenoid cystic carcinoma cells growth and metastasis by blocking the expression of ADAM 10 using RNA interference

10p dauphong4 03-01-2012 63 8   Download

Small interference RNA profiling reveals the essential role of human membrane trafficking genes in mediating the infectious entry of dengue virus

17p dauphong4 24-12-2011 43 4   Download

Tuyển tập báo cáo các nghiên cứu khoa học quốc tế ngành y học dành cho các bạn tham khảo đề tài: Inhibition of Monkeypox virus replication by RNA interference

10p dauphong4 24-12-2011 58 3   Download

Tuyển tập báo cáo các nghiên cứu khoa học quốc tế ngành y học dành cho các bạn tham khảo đề tài: Shortcomings of short hairpin RNA-based transgenic RNA interference in mouse oocytes

10p panasonic01 12-12-2011 50 7   Download