Secretion system

Fibroblast growth factor-2 (FGF-2) is a multifunctional protein that plays an important role in the regulation of proliferation, differentiation and migration of a variety of cells. The recombinant human FGF-2 (rhFGF-2) is currently used in stem cell culture, medicine and cosmetic products. In this study, we aim to produce secreted rhFGF-2 protein from a Pichia pastoris strain containing multiple copies of the fgf-2 gene to eliminate the disadvantages of intracellular expression systems

9p larachdumlanat127 02-01-2021 13 2   Download

phần 1 cuốn sách "tiếng anh công nghệ ô tô và chế tạo máy" trình bày các nội dung: things every driver should know, the way to your cars heart is through your toolbox, preventive maintenance- a monthly under-the-hood check, the inner secrets of your car revealed,... mời các bạn tham khảo.

300p thangnamvoiva8 01-08-2016 135 46   Download

Invite you to consult the document content "Listening practice through dictation 4 - Word list" for more documents serving the academic needs of English vocabulary. Content document you referred to the system vocabulary list, hope this is useful references for you.

9p phamthanhhieu2011 13-11-2015 113 10   Download

Malware: software designed to infiltrate or damage a computer system without the owner's informed consent Spyware: software designed to intercept or take partial control over the user's interaction with the computer, without the user's informed consent secretly monitors the user's behavior collect various types of personal information

33p trinhvietnambk 29-05-2013 64 4   Download

The Rv0948c gene fromMycobacterium tuberculosisH37Rv encodes a 90 amino acid protein as the natural gene product with chorismate mutase (CM) activity. The protein, 90-MtCM, exhibits Michaelis–Menten kinetics with akcat of 5.5 ± 0.2 s )1 and a Kmof 1500 ± 100lmat 37C and pH 7.5. The 2.0 A˚ X-ray structure shows that 90-MtCM is an alla-helical homodimer (Protein Data Bank ID: 2QBV) with the topology ofEscheri-chia coliCM (EcCM), and that both protomers contribute to each catalytic site.

12p research12 29-04-2013 38 3   Download

Humanpancreatic secretions contain twomajor trypsinogen isoforms, cationic and anionic trypsinogen, normally at a ratio of 2 : 1. Pancreatitis, pancreatic cancer and chronic alcoholism lead to a characteristic reversal of the isoform ratio, and anionic trypsinogen becomes the predominant zymogen secreted. To understand the biochemical conse-quences of these alterations, we recombinantly expressed and purified both human trypsinogens and documented characteristics of autoactivation, autocatalytic degradation and Ca 2+ -dependence....

12p fptmusic 16-04-2013 40 3   Download

The mono(ADP-ribosyl)ation reaction is a post-translational modification that is catalysed by both bacterial toxins and eukaryotic enzymes, and that results in the transfer of ADP-ribose from bNAD + to various acceptor proteins. In mammals, both intracellular and extracellular reactions have been described; the latter are due to glycosylphosphatidylinositol-anchored or secreted enzymes that are able to modify their targets, which include the purinergic receptor P2X7, the defensins and the integrins. ...

11p fptmusic 12-04-2013 36 4   Download

pH-Dependent structural changes forEscherichia coliO157:H7 EspA were characterized by CD, 8-anilino-2-naphthyl sulfonic acid (ANS) fluores-cence, and sedimentation equilibrium ultracentrifugation. Far- and near-UV CD spectra, recorded between pH 2.0 and 7.0, indicate that the protein has significant amounts of secondary and tertiary structures. An increase in ANS fluorescence intensity (in the presence of EspA) was observed at aci-dic pH;

0p awards 06-04-2013 36 3   Download

The structural properties of EspB, a virulence factor of theEscherichia coli O157 type III secretion system, were characterized. Far-UV and near-UV CD spectra, recorded between pH 1.0 and pH 7.0, show that the protein assumesa-helical structures and that some tyrosine tertiary contacts may exist. All tyrosine side-chains are exposed to water, as determined by acryl-amide fluorescence quenching spectroscopy.

13p awards 05-04-2013 27 4   Download

Collectins are a family of collagenous calcium-dependent defense lectins in animals. Their polypeptide chains consist of four regions: a cysteine-rich N-terminal domain,a colla-gen-like region,ana-helical coiled-coil neck domain and a C-terminal lectin or carbohydrate-recognition domain. These polypeptide chains form trimers that may assemble into larger oligomers. The best studied family members are the mannan-binding lectin,which is secreted into the blood by the liver,and the surfactant proteins A andD,which are secreted into the pulmonary alveolar and airway lining fluid....

21p dell39 03-04-2013 46 6   Download

We report the secreted expression byPichia pastorisof two human lyso-zyme variants F57I and W64R, associated with systemic amyloid disease, and describe their characterization by biophysical methods. Both variants have a substantially decreased thermostability compared with wild-type human lysozyme, a finding that suggests an explanation for their increased propensity to form fibrillar aggregates and generate disease.

10p dell39 27-03-2013 40 4   Download

Bacillusspecies are valuable producers of industrial enzymes and biophar-maceuticals, because they can secrete large quantities of high-quality pro-teins directly into the growth medium. This requires the concerted action of quality control factors, such as folding catalysts and ‘cleaning proteases’.

12p inspiron33 25-03-2013 40 5   Download

Type III secretion systems of Gram-negative pathogenic bacteria allow the injection of effector proteins into the cytosol of host eukaryotic cells. Cros-sing of the eukaryotic plasma membrane is facilitated by a translocon, an oligomeric structure made up of two bacterial proteins inserted into the host membrane during infection.

10p galaxyss3 19-03-2013 36 4   Download

Among molluscs, the shell biomineralization process is controlled by a set of extracellular macromolecular components secreted by the calcifying mantle. In spite of several studies, these components are mainly known in bivalves from only few members of pteriomorph groups.

13p galaxyss3 19-03-2013 41 3   Download

EspB is a multifunctional protein associated with the type III secretion system of enterohaemorrhagic Escherichia coli, and interacts with various biomolecules includinga-catenin in the host cell. The binding of EspB to a-catenin is thought be involved in actin reorganization during bacterial infection, although the precise mechanism of this phenomenon is still unclear.

8p vinaphone15 28-02-2013 27 4   Download

Triacylglycerol metabolism inSaccharomyces cerevisiae was analyzed quan-titatively using a systems biological approach. Cellular growth, glucose uptake and ethanol secretion were measured as a function of time and used as input for a dynamic flux-balance model.

12p vinaphone15 28-02-2013 38 4   Download

"I am fairly familiar with all the forms of secret writings, and am myself the author of a trifling monograph upon the subject, in which I analyze one hundred and sixty separate ciphers," said Holmes. —The Adventure of the Dancing Men, Sir Arthur Conan Doyle

32p muathu_102 28-01-2013 46 3   Download

traditional private/secret/single key cryptography uses one key Key is shared by both sender and receiver if the key is disclosed communications are compromised also known as symmetric, both parties are equal hence does not protect sender from receiver forging a message & claiming is sent by sender

23p muathu_102 28-01-2013 46 3   Download

To use data encryption algorithms in network communications, all parities must first agree on using the same secret keys Rely on couriers Set up a meeting to determine a secret key Use postal service, email service, phone service … However, these conventional methods are inflexible for network communication applications Public-key cryptography (PKC) Invented in the 1970’s Without the need of sharing prior secrets to distribute secret keys securely Can also be used for authentication

39p muathu_102 28-01-2013 69 4   Download

Cryptography (or cryptology; from Greek κρυπτός, "hidden, secret"; and γράφειν, graphein, "writing", or -λογία, -logia, "study", respectively)[1] is the practice and study of techniques for secure communication in the presence of third parties (called adversaries).[2] More generally, it is about constructing and analyzing protocols that overcome the influence of adversaries

0p doilan 25-01-2013 58 5   Download