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Báo cáo khoa học: A pH-dependent conformational change in EspA, a component of the Escherichia coli O157:H7 type III secretion system

Chia sẻ: Nguyen Thang | Ngày: | Loại File: PDF | Số trang:0

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pH-Dependent structural changes forEscherichia coliO157:H7 EspA were characterized by CD, 8-anilino-2-naphthyl sulfonic acid (ANS) fluores-cence, and sedimentation equilibrium ultracentrifugation. Far- and near-UV CD spectra, recorded between pH 2.0 and 7.0, indicate that the protein has significant amounts of secondary and tertiary structures. An increase in ANS fluorescence intensity (in the presence of EspA) was observed at aci-dic pH;

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Nội dung Text: Báo cáo khoa học: A pH-dependent conformational change in EspA, a component of the Escherichia coli O157:H7 type III secretion system

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