Sequence database

Partitioning DFDs: a. The process of analyzing a DFD and deriving a series of manual procedures b. Adding controls to ensure the processes are done property c. The process of analyzing a DFD and deriving a series manual procedures and computer programs d. None of these answers Câu 162: Physical data flow diagram: a. describing processes in greater detail b. show sequencing processes in the order they must be executed c. show how the system operates or how the new system will be implemented d. all of these answers Câu 163: Physical DFDs a. show how the system is or...

29p itvovantien 23-06-2012 125 20   Download

The objective of the thesis is to propose a solution to mine the weighted sequential patterns between sequences in sequence databases with time interval and quantitative sequence databases with time interval.

25p beloveinhouse01 15-08-2021 15 3   Download

Research purpose: Determine the composition, structure of mitochondrial genome, compare the difference in sequence, identify specific genetic characteristics of the six indigenous pig breeds, thereby contributing to the database to identification and conservation.

27p extraenglish 24-05-2021 26 4   Download

Collecting complete data of mitochondrial genome of 6 indigenous pig breeds in Vietnam (I, Mong Cai, Muong Lay, Huong, Muong Khuong and Ha Lang pigs) and deposited on the Genebank; determine the composition, structure of mitochondrial genome, compare the difference in sequence, identify specific genetic characteristics of the six indigenous pig breeds, thereby contributing to the database to identification and conservation.

27p capheviahe26 25-01-2021 15 4   Download

The present work reports isolation and characterization of a highly glycosylated protein from bovine milk fat globule membranes, known as PAS III. Partial amino-acid sequencing of the purified protein allowed construction of degenerate oligonucleotide primers, enabling isolation of a full-length cDNA encoding a protein of 330 amino-acid residues. N-terminal amino-acid sequencing of derived peptides and the purified protein confirmed 76% of the sequence and demonstrated presence of a cleavable signal peptide of 23 residues, leaving a mature protein of 307 amino acids.

9p system191 01-06-2013 50 4   Download

The siglecs (sialic acid-binding Ig-like lectins) are a distinct subset of the Ig superfamily with adhesion-molecule-like structure. We describe here a novel member of the siglec protein family that shares a similar structure including five Ig-like domains, a transmembrane domain, and a cytoplasmic tail containing two ITIM-signaling motifs. Siglec10 was identified through database mining of an asthmatic eosinophil EST library. Using the Stanford G3 radiation hybrid panel we were able to localize the genomic sequence of siglec-10 within the cluster of genes on chromosome 19q13.

14p system191 01-06-2013 43 6   Download

Angiotensin converting enzyme (ACE) was already discovered in insects in 1994, but its physiological role is still enigmatic. We have addressed this problem by purifying four new ACE substrates from the ovaries of the grey fleshfly, Neobellieria bullata. Their primary structures were identified as NKLKPSQWISLSD (Neb-ODAIF-11)13), NKLKPSQWI (Neb-ODAIF-11)9), SLKPSNWLTPSE (Neb-ODAIF-2) and LEQIYHL. Database analysis showed significant homology with amino acid sequence stretches as present in the N-terminal part of several fly yolk proteins....

9p research12 01-06-2013 37 3   Download

A technique has been developed to search a proteome database for new members of a functional class of membrane protein. It takes advantage of the highly conserved secondary structure of functionally related membrane proteins. Such proteins typically have the same number of transmembrane domains located at similar relative positions in their polypeptide sequence. This gives rise to a characteristic pattern of peaks in their hydropathy profiles.

7p research12 01-06-2013 34 4   Download

Acomprehensive, structural and functional, insilicoanalysis of the medium-chain dehydrogenase/reductase (MDR) superfamily, including 583 proteins, was carried out by use of extensive database mining and theBLASTPprogram in an iterative manner to identify all known members of the superfamily. Based on phylogenetic, sequence, and func-tional similarities, the protein members of the MDR super-family were classified into three different taxonomic categories: (a) subfamilies, consisting of a closed group containing a set of ideally orthologous proteins that perform the same function;...

26p tumor12 20-04-2013 37 6   Download

BLASTanalysis of the human and mouse genome sequence databases using the sequence of the human CMP-sialic acid:b-galactoside a-2,6-sialyltransferase cDNA (hST6Gal I, EC2.4.99.1) as a probe allowed us to identify a putative sialyltransferase gene on chromosome 2. The sequence of the corresponding cDNA was also found as an expressed sequence tag of human brain.

12p tumor12 20-04-2013 28 2   Download

As a first step toward developing a zebrafish model for investigating the role of sulfation in counteracting environ-mental estrogenic chemicals, we have embarked on the identification and characterization of cytosolic sulfotrans-ferases (STs) in zebrafish. By searching the zebrafish expressed sequence tag database, we have identified two cDNA clones encoding putative cytosolic STs. These two zebrafish ST cDNAs were isolated and subjected to nuc-leotide sequencing. Sequence data revealed that the two zebrafishSTs are highlyhomologous, being
82%identical in their amino acid sequences....

8p fptmusic 16-04-2013 42 2   Download

Perhaps the most widely used applications of bioinformatics are tools such as psi-blast for searching sequence databases. We describe a recently developed protein database search algorithm calledrankprop. rankprop relies upon a precomputed network of pairwise protein similarities. The algorithm performs a diffusion operation from a specified query protein across the protein similarity network.

10p fptmusic 12-04-2013 49 3   Download

Almost all protein database search methods use amino acid substitution matrices for scoring, optimizing, and assessing the statistical significance of sequence alignments. Much care and effort has therefore gone into con-structing substitution matrices, and the quality of search results can depend strongly upon the choice of the proper matrix. A long-standing problem has been the comparison of sequences with biased amino acid composi-tions, for which standard substitution matrices are not optimal....

9p fptmusic 12-04-2013 37 2   Download

The 2-hydroxycarboxylate transporter (2HCT) family of secondary trans-porters belongs to a much larger structural class of secondary transporters termed ST3 which contains about 2000 transporters in 32 families. The transporters of the 2HCT family are among the best studied in the class. Here we detect weak sequence similarity between the N- and C-terminal halves of the proteins using a sensitive method which uses a database con-taining the N- and C-terminal halves of all the sequences in ST3 and involves blastsearches of each sequence in the database against the whole database. ...

11p awards 06-04-2013 31 4   Download

The cloning of two novel genes that encode cytokinin oxidase/dehydrogenase (CKX) in barley is described in this work. Transformation of both genes intoArabidopsisand tobacco showed that at least one of the genes codes for a functional enzyme, as its expression caused a cytokinin-deficient phenotype in the heterologous host plants. Addi-tional cloning of two gene fragments, and anin silicosearch in the public expressed sequence tag clone databases, revealed the presence of at least 13 more members of the CKXgene family in barley and wheat. ...

13p awards 05-04-2013 54 4   Download

The ESSS protein is a recently identified subunit of mam-malian mitochondrial complex I. It is a relatively small integral membrane protein (122 amino acids) found in the b-subcomplex. Genomic sequence database searches reveal its localization to theX-chromosome inhumans andmouse. TheESSScDNAfromChinesehamster cellswas clonedand shown to complement one complementation group of our previously described mutants with a proposed X-linkage. Sequence analyses of the ESSS cDNA in these mutants revealed chain termination mutations. ...

9p awards 05-04-2013 36 5   Download

In the postgenomic era it is essential that protein sequences are annotated correctly in order to help in the assignment of their putative functions. Over 1300 proteins in current pro-tein sequence databases are predicted to contain a PAS domainbaseduponaminoacid sequence alignments.Oneof theproblemswith the current annotationof thePASdomain is that this domain exhibits limited similarity at the amino acid sequence level.

11p dell39 03-04-2013 40 5   Download

A homology search of the genome database of the filamentous fungus Trichoderma reeseiidentified a new T. reeseityrosinase gene tyr2, encoding a protein with a putative signal sequence. The gene was overexpressed in the native host under the strong cbh1 promoter, and the tyrosinase enzyme was secreted into the culture supernatant.

14p inspiron33 25-03-2013 48 5   Download

Despite its importance in plant metabolism, no sequences of higher plant ATP-dependent phosphofructokinase (EC 2.7.1.11) are annotated in the databases. We have purified the enzyme from spinach leaves 309-fold to electrophoretic homogeneity. The purified enzyme was a homotetramer of 52 kDa subunits with a specific activity of 600 mUÆmg )1 and aKmvalue for ATP of 81 lm.

10p inspiron33 23-03-2013 47 5   Download

The unique family of membrane-bound proton-pumping inorganic pyro-phosphatases, involving pyrophosphate as the alternative to ATP, was investigated by characterizing 166 members of the UniProtKB⁄Swiss-Prot + UniProtKB⁄TrEMBL databases and available completed genomes, using sequence comparisons and a hidden Markov model based upon a conserved 57-residue region in the loop between transmembrane segments 5 and 6.

11p inspiron33 23-03-2013 43 4   Download