Serological detection
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Part 1 book "Cryptosporidium - Methods and protocols" includes content: Cryptosporidium diagnostic assays - microscopy; cryptosporidium diagnostic assays - molecular detection; detection of cryptosporidium recovered from large volume water samples using dead end ultrafiltration; cryptosporidium oocyst purification using discontinuous gradient centrifugation; measuring cryptosporidium serologic responses by multiplex bead assay,... and other contents.
210p muasambanhan05 16-01-2024 2 0 Download
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The purpose of this study was to compare the diagnostic value of serum oligosaccharide chain (G-test), alpha-fetoprotein (AFP) and aspartic aminotransferase to alanine aminotransferase ratios (AAR), both alone and in combination, for predicting hepatocellular carcinoma (HCC) onset.
11p vialfrednobel 23-12-2023 4 3 Download
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Brucellosis is a zoonosis whose incidence is not declining worldwide despite the global effort to control the disease. Accurate and precise diagnosis is a crucial step in any prophylaxis program but single tests to unequivocally detect animals infected with Brucella spp. are currently unavailable.
7p vigalileogalilei 28-02-2022 11 1 Download
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Brucellosis is an emerging yet neglected zoonosis that has been reported in Kenya. Epidemiological data on brucellosis in ruminants is readily accessible; however, reports on brucellosis in pigs remain limited. This study sought to detect Brucella infection in pig serum by both serological and molecular techniques.
7p vigalileogalilei 28-02-2022 7 1 Download
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The development of sensitive and specific methods for detecting Toxoplasma gondii infection is critical for preventing and controlling toxoplasmosis in humans and other animals. Recently, various recombinant proteins have been used in serological tests for diagnosing toxoplasmosis.
9p vidarwin 23-02-2022 6 1 Download
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Coxiella burnetii causes Q fever, a zoonotic bacterial disease with a multi-host cycle and reservoirs in wild and domestic animal species. Q fever has a significant impact on the Australian public health and economy but its ecology and contributing reservoir species remain poorly understood.
5p vigandhi 23-02-2022 8 1 Download
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In dogs with atopic dermatitis, intradermal testing (IDT) or allergen specific IgE serological testing are routinely employed to identify causative allergens. These allergens can then be used for allergen-specific immunotherapy and allergy management.
10p vidarwin 23-02-2022 8 1 Download
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A novel Brucella strain closely related to Brucella (B.) melitensis biovar (bv) 3 was found in Croatian cattle during testing within a brucellosis eradication programme. Case presentation: Standardised serological, brucellin skin test, bacteriological and molecular diagnostic screening for Brucella infection led to positive detection in one dairy cattle herd.
5p vidarwin 23-02-2022 15 1 Download
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Toxoplasma gondii is an important parasite that belongs to the phylum Apicomplexa, distributed globally, causing major health issues for a wide range of hosts, including humans, native and wild animals.
7p vidarwin 22-02-2022 11 1 Download
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The evolution of influenza A viruses leads to the antigenic changes. Serological diagnosis of the antigenicity is usually labor-intensive, time-consuming and not suitable for early-stage detection.
10p viseulgi2711 31-08-2021 5 1 Download
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We herein compared the diagnostic value of next-generation sequencing (NGS), bacterial culture, and serological biomarkers to detect periprosthetic joint infection (PJI) after joint replacement.
9p vianttinic2711 20-04-2021 7 2 Download
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The aim of this study was to assess whether serology and spirometry and the combination of both can complement culture-based detection for earlier recognition of Pseudomonas aeruginosa infection in children with cystic fibrosis.
10p vimaine2711 26-03-2021 10 2 Download
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Dengue virus serotyping is crucial for clinical management and epidemiology of the disease. As serotype shifts and secondary infection with a heterologous DENV serotype are frequently associated with disease severity, there is a need for sustained monitoring of the circulating serotypes and enhanced surveillance operations to prevent large scale severe dengue outbreaks.
8p chauchaungayxua11 23-03-2021 20 1 Download
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The present study was taken up in order to develop polyclonal antibodies against both the pathogens, for the serological detection of their presence in fruits before fruit ripening.
8p chauchaungayxua11 23-03-2021 8 1 Download
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Health status of laboratory rats indicates its suitability for the researches. They are prone to develop viral infections of subclinical nature caused by parvoviruses, which affects the research results adversely; Hence Implementation of health monitoring protocol is essential at the level of breeding colony itself.
7p trinhthamhodang1215 23-09-2020 14 1 Download
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Pancreatic cancer (PC) is an aggressive disease with an urgent need for biomarkers. Hallmarks of PC include increased collagen deposition (desmoplasia) and increased matrix metalloproteinase (MMP) activity. The aim of this study was to investigate whether protein fingerprints of specific MMP-generated collagen fragments differentiate PC patients from healthy controls when measured in serum.
6p vijennie2711 29-09-2020 18 1 Download
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Brucellosis is a zoonosis known to be a major public health hazard of great economic importance globally. The present study was taken up to ascertain the seroprevalence of brucellosis in goats, sheep, and humans of Anand, districts of the Central Gujarat region. A total of 200 serum samples were collected from the goat and sheep of Anand district and subjected to different serological test i.e., Rose Bengal Plate test (RBPT), Standard Tube Agglutination Test (STAT) and Indirect ELISA to detect the Brucella antibody and also compared in terms of their sensitivity and specificity.
10p cothumenhmong7 09-09-2020 11 1 Download
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Therefore in this study immunodominant region in TP53 gene was amplified and cloned in prokaryotic expression vector. Further the conditions were optimized for the expression of the recombinant protein. The recombinant protein was purified using Ni-NTA affinity chromatography. The hyperimmune sera were raised in rabbits against the recombinant protein. In the hyperimmune sera, antibodies specific to TP53 were detected by Indirect ELISA, confirming the serological reactivity of the immunodominant region.
8p nguaconbaynhay7 15-08-2020 12 2 Download
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Bovine respiratory infection is considered as most important factor of economic losses in dairy herds attributed through calf mortality and reduced performances. Most of the viral etiologies in respiratory infection go unnoticed due to supervene by secondary bacterial infection. Serological presence of antibodies reflects the distribution of infectious agents in herd(s) and may be helpful for designing the prevention strategies in future.
11p chauchaungayxua6 26-06-2020 12 0 Download
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This study attempted to determine the prevalence of HBV infection and comparative assessment between serological and molecular diagnoses among 75 samples divided into three groups as treated, untreated and control groups from different cities in Wasit Province, Iraq, during the period from March 2016 to May 2016. The samples were analyzed by ELISA and Real Time – PCR techniques for detecting the prevalence of HBV in patients.
7p chauchaungayxua6 26-06-2020 14 1 Download