Small cell numbers
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Exome sequencing (ES) has been successfully applied in clinical detection of single nucleotide variants (SNVs) and small indels. However, identification of copy number variants (CNVs) using ES data remains challenging. The purpose of this study is to understand the contribution of CNVs and copy neutral runs of homozygosity (ROH) in molecular diagnosis of patients referred for ES.
17p vibransone 28-03-2024 3 1 Download
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Single-cell genomics is a rapidly advancing field; however, most techniques are designed for mammalian cells. We present a single-cell sequencing pipeline for an intracellular parasite, Plasmodium falciparum, with a small genome of extreme base content.
23p vibransone 28-03-2024 1 1 Download
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Non-small cell lung cancer is currently the second most common cancer when assessing common cancer and the number of cancer deaths; there are a limited number of studies about depression in lung cancer patients in Vietnam. This cross-sectional descriptive study aimed to describe depression in non-small cell lung cancer patients treated at K Hospital from 2021 – 2022 and associated factors.
7p vilarry 12-03-2024 4 1 Download
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Primary lymphoma of the prostate is an exceedingly rare disease, with diffuse large B-cell lymphoma being the most common known subtype in a small number of reported cases. Due to its low prevalence, there has been a chronic lack of targeted diagnostic guidelines and treatment procedures.
5p vilazada 31-01-2024 2 2 Download
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Ebook "The retinoblastoma protein" covers the mechanisms of pRb inactivation detailing repressive mechanisms commonly associated to cancer, and representative of the experimentally relevant tests used in the establishment of cancer diagnosis and prognosis. Chapters contain protocols and in-depth discussions for commonly used experimental approaches to assess the status and function of components of the pRb pathway, including pRb itself, in cell lines and biological samples.
205p lucchinguyen 28-12-2023 5 2 Download
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Epidermal growth factor receptor (EGFR) amplifcation refers to the copy number increase of EGFR gene, and is often identified as a “bypass” way of Epidermal growth factor receptor Tyrosine kinase inhibitors (EGFRTKI) resistance. We aimed to explore the effect of EGFR amplification on EGFR mutation treatment-naive advanced non-squamous non-small cell lung cancer (NSCLC) patients.
10p vileonardodavinci 23-12-2023 3 2 Download
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Immunotherapy targeting the PD-1/PD-L1 pathway is a standard of care in a number of metastatic malignancies, but less than a fifth of patients are expected to respond to ICIs (Immune Checkpoint Inhibitors). In a clinical trial, combining the anti-TIGIT (T cell immunoreceptor with Ig and ITIM domains) Mab (monoclonal antibody) tiragolumab with atezolizumab improved outcomes in non-small cell lung cancer.
15p vialfrednobel 23-12-2023 8 4 Download
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Ebook "Introduction to percolation theory (2nd revised edition)" dealing with percolation theory clustering, criticallity, diffusion, fractals and phase transitions takes a broad approach to the subject, covering basic theory and also specialized fields like disordered systems and renormalization groups.
179p loivantrinh 29-10-2023 3 2 Download
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As the dominant component of the tumor microenvironment, cancer-associated fibroblasts (CAFs), play a vital role in tumor progression. An increasing number of studies have confirmed that CAFs are involved in almost every aspect of tumors including tumorigenesis, metabolism, invasion, metastasis and drug resistance, and CAFs provide an attractive therapeutic target.
15p vischultz 20-10-2023 5 2 Download
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Most cases of lung cancer are diagnosed at advanced stage. Detection of genetic and epigenetic mark‑ ers in cell-free DNA (cfDNA) is a promising tool for the diagnosis of lung cancer at an early stage. The aim of this study was to identify non-invasive diagnostic markers in cell free DNA (cfDNA) for non-small cell lung cancer (NSCLC) as it is the most common type of lung cancer.
9p vischultz 20-10-2023 3 1 Download
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Many previous studies have demonstrated that minor-frequency pretreatment T790M mutation (preT790M) could be detected by ultrasensitive methods in a considerable number of treatment-naïve, epidermal growth factor receptors (EGFR)-mutated, non-small cell lung cancer (NSCLC) cases.
11p vikolindagrabar 27-07-2022 5 1 Download
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Investigating dynamic changes in blood-parameters and weight in patients with locally advanced non-small cell lung cancer (NSCLC) receiving durvalumab maintenance therapy after chemoradiotherapy (cCRT). Laboratory outcomes were determined based on the number of durvalumab administrations received.
9p vikolindagrabar 27-07-2022 5 1 Download
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Multiple myeloma remains an incurable disease with multiple relapses due to residual myeloma cells in the bone marrow of patients after therapy. Presence of small number of cancer cells in the body after cancer treatment, called minimal residual disease, has been shown to be prognostic for progression-free and overall survival.
11p vipriyankagandhi 27-07-2022 9 1 Download
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Spatial mapping of genomic data to tissue context in a high-throughput and high-resolution manner has been challenging due to technical limitations. Here, we describe PHLI-seq, a novel approach that enables high-throughput isolation and genome-wide sequence analysis of single cells or small numbers of cells to construct genomic maps within cancer tissue in relation to the images or phenotypes of the cells.
17p vigalileogalilei 27-02-2022 9 1 Download
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The initiation and subsequent evolution of cancer are largely driven by a relatively small number of somatic mutations with critical functional impacts, so-called driver mutations. Identifying driver mutations in a patient’s tumor cells is a central task in the era of precision cancer medicine.
17p vielonmusk 30-01-2022 40 0 Download
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Post-transcriptional regulation of gene expression can be achieved through the control of mRNA stability, cytoplasmic compartmentalization, 3′ UTR length and translational efficacy. Spermiogenesis, a process through which haploid male germ cells differentiate into spermatozoa, represents an ideal model for studying posttranscriptional regulation in vivo because it involves a large number of transcripts that are physically sequestered in ribonucleoprotein particles (RNPs) and thus subjected to delayed translation.
18p vialfrednobel 29-01-2022 5 0 Download
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Synchronous oligometastatic non-small cell lung cancer (NSCLC) is generally characterised by the limited number of metastases at the time of diagnosis. Several clinical trials have shown that local ablative therapy (LAT) at all sites of the disease might be beneficial for patients with oligometastatic NSCLC.
6p vimahuateng 26-11-2021 7 0 Download
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In this study we aimed to characterize the genomic architecture of NSCLC tumors and to identify genomic differences between tumors stratified by histology and mutation status. Furthermore, we sought to integrate DNA copy number data with mRNA expression to find genes with expression putatively regulated by copy number aberrations and the oncogenic pathways associated with these affected genes.
12p vimahuateng 26-11-2021 4 1 Download
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ATAC-seq (Assays for Transposase-Accessible Chromatin using sequencing) is a recently developed technique for genome-wide analysis of chromatin accessibility. Compared to earlier methods for assaying chromatin accessibility, ATAC-seq is faster and easier to perform, does not require cross-linking, has higher signal to noise ratio, and can be performed on small cell numbers.
13p vibeauty 23-10-2021 6 0 Download
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Transgenic zebrafish lines with the expression of a fluorescent reporter under the control of a celltype specific promoter, enable transcriptome analysis of FACS sorted cell populations. RNA quality and yield are key determinant factors for accurate expression profiling.
16p visilicon2711 20-08-2021 11 1 Download