Snake venom

The objectives of the thesis: Research in the toxin/protein having bioactive isolated from Heterometrus laoticus (H. laoticus) scorpion and krait Bungarus fasciatus (B. fasciatus) snake venom.

32p extraenglish 24-05-2021 13 3   Download

The objectives of the thesis: Research in the toxin/protein having bioactive isolated from Heterometrus laoticus (H. laoticus) scorpion and krait Bungarus fasciatus (B. fasciatus) snake venom.

32p extraenglish 24-05-2021 20 3   Download

Venoms from crotalid and viperid snakes contain several peptide inhibitors which regulate the proteolytic activities of their snake-venom metalloproteinases (SVMPs) in a reversible manner under physiological conditions. In this report, we describe the high-resolution crystal structures of a SVMP, TM-3, from Taiwan habu (Trimeresurus mucrosquamatus) cocrystallized with the endogenous inhibitors pyroGlu-Asn-Trp (pENW), pyroGlu-Gln-Trp (pEQW) or pyroGlu-Lys-Trp (pEKW).

10p system191 01-06-2013 29 5   Download

In this study, we isolated a 25-kDa novel snake venom protein, designated ablomin, from the venom of the Japanese Mamushi snake (Agkistrodon blomhoffi). The amino-acid sequence of this protein was determined by peptide sequencing and cDNA cloning. The deduced sequence showed high similarity to helothermine from the Mexican beaded lizard (Heloderma horridum horridum), which blocks voltage-gated calcium and potassium channels, and ryanodine receptors. Ablomin blocked contraction of rat tail arterial smooth muscle elicited by high K+-induced depolarization in the 0.

8p system191 01-06-2013 26 3   Download

Weak neurotoxins from snake venom are small proteins with five disulfide bonds, which have been shown to be poor bindersof nicotinicacetylcholine receptors.We report on the cloning and sequencing of four cDNAs encoding weak neurotoxins fromNaja sputatrixvenomglands. The protein encoded by one of them, Wntx-5, has been synthesized by solid-phase synthesis and characterized.

10p research12 23-04-2013 43 4   Download

Bothrops snake venoms are known to induce local tissue damage suchas hemorrhage andmyonecrosis. Theopossum Didelphis marsupialisis resistant to these snake venoms and has natural venom inhibitors in its plasma. The aim of this work was to clone and study the chemical, physicochemical andbiological properties ofDM64, an antimyotoxic protein from opossum serum. DM64 is an acidic protein showing 15% glycosylation andwith amolecular mass of 63 659 Da when analysed by MALDI-TOF MS.

11p tumor12 22-04-2013 36 4   Download

Ammodytoxins (Atxs) are presynaptically acting snake venom phospholipase A2 (PLA2) toxins the molecular mechanism of whose neurotoxicity is not completely understood. Two chimeric PLA2s were prepared by repla-cing the C-terminal part of a nontoxic venom PLA2 , ammodytin I2, with that of AtxA(K108N). The chimeras were not toxic, but were able to bind strongly to an Atxs-specific neuronal receptor, R25.

6p tumor12 22-04-2013 35 2   Download

The molecular mechanism of the presynaptic neurotoxicity of snake venom phospholipases A2(PLA2s) is not yet fully elucidated. Recently, newhigh-affinity binding proteins for PLA2 toxins have been discovered, including the important intracellular Ca 2+ sensor, calmodulin (CaM). In the present study, the mode of interaction of group IIAPLA2swiththe Ca 2+ -bound form of CaM was investigated by mutational analysis of ammodytoxin A (AtxA) from the long-nosed viper (Vipera ammodytes ammodytes).

8p fptmusic 16-04-2013 35 3   Download

The cardiotoxin (cytotoxin II, or CTII) isolated from cobra snake (Naja oxiana) venom is a 60-residue basic membrane-activeprotein featuring three-fingerbetasheet fold.Toassess possible modes of CTII/membrane interaction 31 P- and 1 H-NMR spectroscopy was used to study binding of the toxin and its effect onto multilamellar vesicles (MLV) composed of either zwitterionic or anionic phospholipid, dipalmitoylglycerophosphocholine (Pam2 Gro-PCho) or dipalmitoylglycerophosphoglycerol (Pam2 Gro-PGro), res-pectively. ...

9p fptmusic 16-04-2013 42 3   Download

Comparison of the crystal structures of threeMicropechis ikahekaphos-pholipase A2 isoenzymes (MiPLA2, MiPLA3 and MiPLA4, which exhibit different levels of pharmacological effects) shows that their C-terminus (residues 110–124) is the most variable. M-Type receptor binding affinity of the isoenzymes has also been investigated and MiPLA4 binds to the rabbit M-type receptor with high affinity. Examination of surface charges of the isoenzymes reveals a trend of increase in positive charges with potency....

10p awards 05-04-2013 43 4   Download

There are different glycosylated proteins in snake venoms, but no glycosylated representatives of a large family of three-fingered toxins have previously been detected. A new glycoprotein was isolated from the venom of the Thai cobra Naja kaouthia. MALDI MS of the glycoprotein contained an array of peaks in the range from
8900 to
9400 Da indicating its microheterogeneity. Carbohy-drate analysis showed the presence of mannose, galactose, N-acetylglucosamine, fucose and neuraminic acid.

10p dell39 03-04-2013 37 2   Download

Snake venom metalloproteinases (SVMPs) have recently been shown to interact with proteins containing von Willebrand factor A (VWA) domains, including the extracellular matrix proteins collagen XII, collagen XIV, mat-rilins 1, 3 and 4, and von Willebrand factor (VWF) via their cysteine-rich domain.

11p galaxyss3 19-03-2013 42 4   Download

The three-dimensional structures of some components of snake venoms forming so-called ‘three-fingered protein’ domains (TFPDs) are similar to those of the ectodomains of activin, bone morphogenetic protein and trans-forming growth factor-breceptors, and to a variety of proteins encoded by the Ly6andPlaurgenes.

19p galaxyss3 07-03-2013 41 4   Download

As more data are generated from proteome and transcriptome analyses of snake venoms, we are gaining an appreciation of the complexity of the venoms and, to some degree, the various sources of such complexity. How-ever, our knowledge is still far from complete.

15p galaxyss3 07-03-2013 44 2   Download

Textilinin-1 is a Kunitz-type serine protease inhibitor isolated from the venom of the Australian common brown snake,Pseudonaja textilis. This molecule binds to and blocks the activity of a range of serine proteases, including plasmin and trypsin. Textilinin-1’s ability to inhibit plasmin, a protease involved in fibrinolysis, has raised the possibility that it could be used as an alternative to aprotinin (Trasylol) as a systemic antibleeding agent in surgery.

13p viettel02 22-02-2013 41 2   Download

Snake venoms are cocktails of enzymes and non-enzymatic proteins used for both the immobilization and digestion of prey. The most common snake venom enzymes include acetylcholinesterases, L-amino acid oxidases, serine proteinases, metalloproteinases and phospholipases A2

33p cosis54 04-01-2013 40 5   Download

Hypothesis of snake and insect venoms against Human Immunodeficiency Virus: a review

5p toshiba23 18-11-2011 35 1   Download