Solid medicine

Properties of materials, classes of materials used in medicine,... As the main contents of the lectures "Biomaterials". Invite you to consult for additional documents for the academic needs and research.

21p thangdambme 23-10-2015 46 3   Download

Amplification of N-myc oncogene is a frequent event in advanced stages of human neuroblastoma and correlates with poor prognosis and enhanced neovascularization. Angiogenesis is an indispensable prerequisite for the progression and metastasis of solid malignancies, which is modulated by tumor suppressors and oncogenes. We have addressed the possibility that N-myc oncogene might regulate angiogenesis in neuroblastoma. Here, we report that experimental N-Myc overexpression results in down-regulation of leukemia inhibitory factor (LIF), a modulator of endothelial cell proliferation....

10p system191 01-06-2013 35 4   Download

Decorin is a small leucine-rich chondroitin/dermatan sulfate proteoglycan reported to interact with ®brillar collagens through its protein core and to localize atdandebands of the collagen ®bril banding pattern. Using a solid-phase assay, we have determined the interaction of peptides derivedbyCNBr cleavage of type I and type II collagenwith decorin extracted from bovine tendon and its protein core and with a recombinant decorin preparation.

10p research12 29-04-2013 37 3   Download

Weak neurotoxins from snake venom are small proteins with five disulfide bonds, which have been shown to be poor bindersof nicotinicacetylcholine receptors.We report on the cloning and sequencing of four cDNAs encoding weak neurotoxins fromNaja sputatrixvenomglands. The protein encoded by one of them, Wntx-5, has been synthesized by solid-phase synthesis and characterized.

10p research12 23-04-2013 44 4   Download

A new human/rat CRH analogue has been synthesized using the Fmoc/tBu solid-phase synthetic protocol. The sequence ofthe new peptide differs from the original in two positions, 12 and 15, at which the native amino acids L-phenylalanine 12 and L-leucine 15 have been replaced by the nonprotein amino acids D-phenylalanine and a-aminoisobutyric acid (Aib), respectively. The high resolution three-dimensional solution structure of [D-Phe12, Aib15]CRH has been determined by 688 dis-tance constraints (656 meaningful NOE and 32 H-bonds distance limits) and 21 angle constraints. ...

11p tumor12 22-04-2013 34 3   Download

The multisubstrate nucleoside kinase of Drosophila melanogaster(Dm-dNK) can be expressed in human solid tumor cells and its unique enzymatic properties makes this enzyme a suicide gene candidate. In the present study,Dm-dNK was stably expressed in the CCRF-CEM and H9 T-lymphoblastoid cell lines. The expressed enzyme was localized to the cell nucleus and the enzyme retained its activity.

11p fptmusic 11-04-2013 41 2   Download

Human cytochrome P450 3A4 forms a series of minor testosterone hydroxylation products in addition to 6b-hy-droxytestosterone, the major product. One of these, formed at the next highest rate after the 6b-and 2b-hydroxy prod-ucts, was identified as 1b-hydroxytestosterone. This product was characterized from a mixture of testosterone oxidation products using an HPLC-solid phase extraction-cryoprobe NMR/time-of-flight mass spectrometry system, with an estimated total of
6lgof this product.Mass spectrometry established the formula as C19H29O3(MH + 305.2080)....

8p awards 05-04-2013 41 4   Download

Microfibrils and elastin are major constituents of elastic fibers, the assembly of which is dictated by multimolecular interactions.Microfibril-associated glycoprotein-1 (MAGP-1) is a microfibrillar component that interacts with the sol-uble elastin precursor, tropoelastin. We describe here the adaptation of a solid-phase binding assay that defines the effect of divalent cations on the interactions between MAGP-1 and tropoelastin. Using this assay, a strong cal-cium-dependent interaction was demonstrated, with a dis-sociation constant of 2.8 ± 0.3 nM, which fits a single-site binding model.

0p awards 05-04-2013 42 1   Download

The introduction of noncanonical amino acids and bio-physical probes into peptides and proteins, and total or segmental isotopic labelling has the potential to greatly aid thedeterminationofproteinstructure, functionandprotein– protein interactions.To obtain a peptide as large as possible by solid-phase peptide synthesis, native chemical ligation was introduced to enable synthesis of proteins of up to 120 amino acids in length.

15p dell39 03-04-2013 43 4   Download

The coat proteins of filamentous phage are first synthesized as transmem-brane proteins and then assembled onto the extruding viral particles. We investigated the transmembrane conformation of the Pseudomonas aerugi-nosa Pf3 phage coat protein using proton-decoupled 15 N and 31 P solid-state NMR spectroscopy.

12p dell39 27-03-2013 41 4   Download

We utilized plasmon-waveguide resonance (PWR) spectroscopy to follow the effects of sphingomyelin, cholesterol and zinc ions on the binding and aggregation of the amyloidbpeptide1)40 in lipid bilayers. With a dioleoyl-phosphatidylcholine (DOPC) bilayer, peptide binding was observed, but no aggregation occurred over a period of 15 h.

14p inspiron33 26-03-2013 45 5   Download

The enzymatic kinetics of glycoside hydrolase family 7 cellobiohydrolase (Cel7A) towards highly crystalline celluloses at the solid–liquid interface was evaluated by applying the novel concept of surface density (q) of the enzyme, which is defined as the amount of adsorbed enzyme divided by the maximum amount of adsorbed enzyme.

10p inspiron33 25-03-2013 58 4   Download

The membrane binding affinity of the pleckstrin homology (PH) domain of phospholipase C (PLC)-d1 was investigated using a vesicle coprecipitation assay and the structure of the membrane-associated PH domain was probed using solid-state 13 C NMR spectroscopy.

11p inspiron33 23-03-2013 48 4   Download

Cellulose and wheat straw degradation byRuminococcus albuswas moni-tored using NMR spectroscopy. In situ solid-state 13 C-cross-polarization magic angle spinning NMR was used to monitor the modification of the composition and structure of cellulose and 13 C-enriched wheat straw during the growth of the bacterium on these substrates.

9p galaxyss3 07-03-2013 39 3   Download

Solid tumours are complex structures in which the interdependent relation-ship between tumour and endothelial cells modulates tumour development and metastasis dissemination. The tumour microenvironment plays an important role in this cell interplay, and changes in its features have a major impact on tumour growth as well as on anticancer therapy respon-siveness.

12p galaxyss3 07-03-2013 46 2   Download

The chaperone calreticulin has been suggested to function as a C1q and collectin receptor. The interaction of calreticulin with mannan-binding lectin (MBL) was investigated by solid-phase binding assays. Calreticulin showed saturable and time-dependent binding to recombinant MBL, pro-vided that MBL was immobilized on a solid surface or bound to mannan on a surface.

12p media19 05-03-2013 37 2   Download

Aberrant differentiation is a characteristic feature of neoplastic transforma-tion, while hypoxia in solid tumors is believed to be linked to aggressive behavior and poor prognosis. However, the possible relationship between hypoxia and differentiation in malignancies remains poorly defined.

14p vinaphone15 27-02-2013 36 1   Download

The A-minor interaction, formed between single-stranded adenosines and the minor groove of a receptor helix, is among the most common motifs found in rRNA. Among the A-minors found in 16S rRNA are a set of interactions between adenosines at positions 1433, 1434 and 1468 in helix 44 (h44) and their receptors in the nucleotide 320–340 region of helix 13 (h13).

8p cosis54 09-12-2012 31 2   Download

Tuyển tập báo cáo các nghiên cứu khoa học quốc tế ngành y học dành cho các bạn tham khảo đề tài: Biomarkers in solid organ transplantation: establishing personalized transplantation medicine

12p panasonic02 15-12-2011 33 3   Download