Somaclonal variation

Ebook "Plant developmental biology - Biotechnological perspectives (Volume 2)" focuses on development-related areas, including tissue culture (somatic embryogenesis, microspore embryogenesis, somaclonal variation), plant processes (photosynthesis, seed maturation and seed proteins, fatty acids, vitamins, alkaloids, flower pigments and scent), signalling (amino compound-containing lipids, auxins, cytokinins and light), and molecular genetics of developmental regulation (RNA silencing, DNA methylation, epigenetics, activation tagging, homologous recombination and synthetic promoter engineering).

432p tachieuhoa 28-01-2024 6 2   Download

Part 1 of ebook "Plant biotechnology (Volume 1: Principles, techniques, and applications)" provide readers with content about: history, scope, and importance of plant biotechnology; plant tissue culture; history of biotechnology; scope and importance of plant biotechnology in crop improvement; embryo culture and endosperm culture;... Please refer to the part 1 of ebook for details!

257p langmongnhu 14-12-2022 8 3   Download

Continued part 1, part 2 of ebook "Plant biology and biotechnology (Volume II: Plant genomics and biotechnology)" provide readers with content about: applications of triploids in agriculture; improving secondary metabolite production in tissue cultures; somaclonal variation in micropropagated plants; in vitro conservation of plant germplasm;... Please refer to the part 2 of ebook for details!

375p langmongnhu 14-12-2022 10 3   Download

Somatic variation is a valuable source of trait diversity in clonally propagated crops. In grapevine, which has been clonally propagated worldwide for centuries, important phenotypes such as white berry colour are the result of genetic changes caused by transposable elements.

15p vitzuyu2711 29-09-2021 9 1   Download

Orchids produce a colorless protocorm by symbiosis with fungi upon seed germination. For mass production of orchids, the prevailing approaches are both generation of protocorm-like bodies (PLBs) from callus and multiplication of adventitious buds on inflorescence.

13p vigiselle2711 30-08-2021 13 1   Download

The banana (Musa sp., AAA) genome is constantly increasing due to high-frequency of somaclonal variations. Due to its large diversity, a conventional numerical and morphological based taxonomic identification of banana cultivars is laborious, difficult and often leads to subject of disagreements.

11p vijeeni2711 30-06-2021 12 1   Download

The in vitro raised plantlets were successfully acclimatized under ex vitro conditions and grew well in the glasshouse. All the regenerated plantlets appeared normal with respect to morphological characteristics with 100% survival rate. Somaclonal variation in tissue culture is a common phenomenon which makes it mandatory to check for genetic stability of plants. The genetic variation in the in vitro raised plants was confirmed using Inter Simple Sequence Repeats(ISSR) markers. Comparison of the bands with the mother plant revealed that the genetic variation frequency reached 6.

11p trinhthamhodang11 27-04-2021 16 1   Download

Presently marker linked to resistance for Dagger nematode and pierce disease at university of California in the breeding programs are routinely used for grapevine rootstock improvement. Genome sequencing has unveiled the mystery about various genes, the phylogenetic relationship and evolution history of sequenced fruit crop.

11p cothumenhmong9 18-01-2021 11 2   Download

Genetic uniformity of in vitro raised plants is a prerequisite for production of quality planting material of banana. Embryogenic cultures were induced from immature male flower buds of two important cultivars of banana, ‘Rasthali’ (Silk-Musa AAB) and ‘Grand Naine’ (Cavendish-Musa AAA). Cell suspensions were established and regenerated plants were evaluated for their genetic stability using 10 inter simple sequence repeat markers.

11p angicungduoc6 20-07-2020 9 1   Download

Detection of somaclonal variation in tissue cultured plants is essential for conservation of elite varieties and also to avoid undesired characters. The genetic fidelity of in vitro regenerated Chrysanthemum cv. Pusa centenary, a commercially important cultivar was assessed using RAPD and ISSR markers.

14p quenchua6 15-06-2020 10 2   Download

The experiment was taken up to elicit the information on yield attributes and yield of different ginger (Zingiber officinale Rosc.) somaclones. Ten somaclones of ginger were evaluated in RBD with three replications during 2015-18 at College of Horticulture, Kerala Agricultural University, Thrissur district, Kerala. The growth performance of ten somaclones indicated significant variation at all the stages of crop growth. The somaclones SE 86102 (16.00 days) and SE 8626 (16.33 days) registered least number of days for sprouting.

7p nguathienthan5 04-06-2020 19 1   Download

Thirteen groups of somaclones (seven in Maran and six in Rio-de-Janeiro) were evaluated using Random Amplified Polymorphic DNA (RAPD). RAPD analysis using twelve selected primers produced 129 amplicons, 44 were polymorphic with an average of 3.66 polymorphic bands / primer and a polymorphism percentage of 34.10. The dendrograms generated based on RAPD profiles grouped the somaclones into two separate clusters. RAPD marker system showed that somaclones derived from cultivar Maran exhibited more variability than Rio-de-Janeiro.

8p kethamoi5 03-06-2020 22 0   Download

In this study, RAPD fingerprinting has been carried out for variants selected from orchards growing with micropropagated Robusta banana (Musa spp. ‘AAA’) plants. After isolation of quality DNA and preliminary screening of random primers, fourteen arbitrary (10-mers) primers showing intense unambiguous and reproducible PCR amplification have been selected for study. Among them OPA-19 and OPC-03 showed only monomorphic bands while OPA-02, OPA-09, OPA-13, OPA-14, OPB-6, OPB-15, OPC-01, OPD-10, OPF-04 and OPF-12 primers showed polymorphic bands.

10p nguaconbaynhay5 16-05-2020 11 0   Download

DNA was extracted from plants and RAPD analysis was performed for fourteen treatments with two random oligonucleotide primers (OPA-01 and OPB-11). Among fourteen treatments, plants derived from the treatments T9, T11, and T12 produced polymorphism T12 was found to exhibit maximum polymorphism at 1.0 µM EMS in 2009T5. The clone, 2009T5 produced more variation than 2008T42. Sodium nitrite did not produce any variation.

8p chauchaungayxua5 08-05-2020 15 1   Download

The safety of mutagenized and genetically transformed plants remains a subject of scrutiny. Data gathered and communicated on the phenotypic and molecular variation induced by gene transfer technologies will provide a scientific-based means to rationally address such concerns.

13p viuchiha2711 21-04-2020 11 1   Download

The present study was carried out to monitor somaclonal variations of banana cv. Ney Poovan (AB) during micropropagation using random amplified polymorphic DNA (RAPD). Sword suckers were used as explants and regenerated on MS media supplemented with BAP alone, BAP + Kinetin and TDZ at varying levels of concentrations. The regenerated shoots were again subcultured on MS medium fortified with plant growth regulators at different concentrations. Rooting was obtained on ½ MS medium supplemented with 2.00 mg/l IBA and 2 g/l of activated charcoal.

12p trinhthamhodang4 22-03-2020 15 1   Download

Present investigation was carried out to screen two promising sugarcane varieties CoN 13073 and CoN 09072 for salinity tolerance through callus culture. Various physiological and morphological parameters was studied, maximum shoot length was observed at 2.0 % NaCl concentration treated to callus at 65 days of culturing in variety CoN 13073, similar trend was observed for root length and leaf number per plant in variety CoN 13073 at 2.0 % NaCl concentration..

9p caygaocaolon2 14-03-2020 11 1   Download

Micropropagation is a highly sought after technique in the commercial production of orchid plants. This has the advantage of providing large number of plants in a short period of time. But a major constrain in the in vitro propagation technique is the somaclonal variation. It is important to produce true to type planting materials especially in case of the hybrids as they are more prone to variations. The true to type nature of the micropropagated plantlets can be confirmed by genetic fidelity analysis.

9p caygaocaolon3 27-02-2020 12 2   Download

Tissue culture regenerated materials of Musa L. spp. (banana and plantain) along with their somaclonal variants were assessed for karyotype abnormalities to be used as indicators for rapid cytological marking. The results show that although slight variations were noted in karyomorphometric characters, like chromosome number, arm length, total chromosome length, and total chromosome volume, these were not significant (P ≤ 0.05).

5p vibasque27 29-03-2019 21 0   Download

Chrysanthemum (Chrysanthemum × grandiflorum /Ramat./Kitam.) is the second most popular ornamental plant in the global flower industry, and there is still a demand for novelty, which forces breeders to search for new sources of variation.

9p caplock2711 21-02-2019 24 1   Download