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Splice junction
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Gene annotations, such as those in GENCODE, are derived primarily from alignments of spliced cDNA sequences and protein sequences. The impact of RNA-seq data on annotation has been confined to major projects like ENCODE and Illumina Body Map 2.0.
14p
vialfrednobel
29-01-2022
10
0
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Transcriptome assemblers aim to reconstruct full-length transcripts from RNA-seq data. We present TransComb, a genome-guided assembler developed based on a junction graph, weighted by a bin-packing strategy and pairedend information. A newly designed extension method based on weighted junction graphs can accurately extract paths representing expressed transcripts, whether they have low or high expression levels.
9p
viaristotle
29-01-2022
12
0
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Alternative splicing, which generates multiple mRNA isoforms from single genes, is crucial for the regulation of eukaryotic gene expression. The flux through competing splicing pathways cannot be determined by traditional RNA-Seq, however, because different mRNA isoforms can have widely differing decay rates.
21p
viarchimedes
26-01-2022
13
0
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Exon splicing is a regulated cellular process in the transcription of protein-coding genes. Technological advancements and cost reductions in RNA sequencing have made quantitative and qualitative assessments of the transcriptome both possible and widely available.
13p
viseulgi2711
31-08-2021
13
1
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The DGAT1 gene encodes an enzyme responsible for catalysing the terminal reaction in mammary triglyceride synthesis, and underpins a well-known pleiotropic quantitative trait locus (QTL) with a large influence on milk composition phenotypes.
13p
vijeeni2711
30-06-2021
10
1
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Next generation transcriptome sequencing (RNA-Seq) is emerging as a powerful experimental tool for the study of alternative splicing and its regulation, but requires ad-hoc analysis methods and tools.
11p
viwyoming2711
16-12-2020
17
1
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TopHat is a popular spliced junction mapper for RNA sequencing data, and writes files in the BAM format – the binary version of the Sequence Alignment/Map (SAM) format. BAM is the standard exchange format for aligned sequencing reads, thus correct format implementation is paramount for software interoperability and correct analysis.
3p
vioklahoma2711
19-11-2020
14
1
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This study presents the first molecularly proven data on natural hybridization in the genus Pulsatilla, including the complete plastome sequence. A plant with morphological characteristics intermediate between P. patens and P. pratensis was found near the village of Bocheniec (southern Poland).
9p
caplock2711
21-02-2019
17
1
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We used a combined computer and biochemical approach to characterize human pyridoxine 5¢-phosphate oxidase (PNPO).The human PNPOgene is composed of seven exons and six introns, and spans approximately 8 kb.All exon/intron junctions contain the gt/ag consensus splicing site.The absence of TATA-like sequences, the presence of Sp1-binding sites and more importantly, the presence of CpG islands in the regulatory region of thePNPOgene are characteristic features of housekeeping genes.
10p
dell39
03-04-2013
46
2
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Protein phosphorylation ensures the accurate and controlled expression of the genome, for instance by regulating the activities of pre-mRNA splicing factors. Here we report that splicing factor 1 (SF1), which is involved in an early step of intronic sequence recognition, is highly phosphorylated in mam-malian cells on two serines within an SPSP motif at the junction between its U2AF 65 and RNA binding domains.
11p
dell39
27-03-2013
33
3
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The tight junction protein occludin participates in cell adhesion and migra-tion and has been shown to possess antitumorigenic properties; however, the exact mechanism underlying these effects is poorly understood. In liver cell lines, we identified an occludin splice variant deleted in exon 9 (Occ DE9 ).
12p
galaxyss3
07-03-2013
45
2
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Tuyển tập các báo cáo nghiên cứu về y học được đăng trên tạp chí y học Wertheim cung cấp cho các bạn kiến thức về ngành y đề tài: Global and unbiased detection of splice junctions from RNA-seq data...
0p
thulanh21
15-11-2011
43
1
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ADC’s FiberGuide® Fiber Management Systems offer the greatest breadth of optical raceway products in the industry. In response to customer requirements, ADC continues to innovate and improve FiberGuide Systems, adding greater flexibility and driving down installation time to ensure a smooth deployment. FiberGuide is a raceway system designed to protect and route fiber optic patch cords, multi-fiber cable assemblies and intrafacility fiber cable (IFC) to and from fiber splice enclosures, fiber distribution frames and fiber optic terminal devices.
18p
halanh
14-08-2009
102
4
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ADC’s FiberGuide® Fiber Cable Management System is a trough system designed to protect and route fiber optic patch cords, multifiber cable assemblies, and intrafacility fiber cable (IFC) to and from fiber splice enclosures, fiber distribution frames, and fiber optic terminal devices. The FiberGuide system is designed to ensure a 2-inch minimum bend radius is maintained throughout the system. The FiberGuide system is a complete set of products designed and manufactured to ensure total off-frame protection.
16p
thuyvan
13-08-2009
97
5
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