Starch binding domain
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Starch-binding domains from carbohydrate binding module family 20 have been used as a tool for starch engineering. Previous studies showed that expression of starch binding domain fusion proteins in planta resulted in modified starch granule structures and physicochemical properties.
8p vihamax2711 21-04-2020 14 0 Download
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The a-amylase family (glycoside hydrolase family 13; GH 13) contains enzymes with approximately 30 specifi-cities. Six types of enzyme from the family can possess a C-terminal starch-binding domain (SBD): a-amylase, maltotetraohydrolase, maltopentaohydrolase, maltogenic a-amylase, acarviose transferase, and cyclodextrin glu-canotransferase (CGTase). Such enzymes are multidomain proteins and those that contain an SBD consist of four or five domains, the former enzymes being mainly hydrolases and the latter mainly transglycosidases. ...
11p tumor12 20-04-2013 32 1 Download
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Approximately 10% of amylolytic enzymes are able to bind and degrade raw starch. Usually a distinct domain, the starch-binding domain (SBD), is responsible for this property. These domains have been classified into families of carbohydrate-binding modules (CBM). At present, there are six SBD families: CBM20, CBM21, CBM25, CBM26, CBM34, and CBM41.
17p fptmusic 12-04-2013 34 3 Download
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Most glucoamylases (a-1,4-d-glucan glucohydrolase, EC 3.2.1.3) have structures consisting of both a catalytic and a starch binding domain. The structure of a glucoamylase from Saccharomycopsis fibuligeraHUT 7212 (Glu), determined a few years ago, consists of a single catalytic domain. The structure of this enzyme with the resolution extended to 1.1 A˚ and that of the enzyme–acarbose complex at 1.6 A˚ resolution are presented here.
11p inspiron33 26-03-2013 54 5 Download
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Some starch-degrading enzymes accommodate carbohydrates at sites situ-ated at a certain distance from the active site. In the crystal structure of barleya-amylase 1, oligosaccharide is thus bound to the ‘sugar tongs’ site. This site on the non-catalytic domain C in the C-terminal part of the mole-cule contains a key residue, Tyr380, which has numerous contacts with the oligosaccharide.
13p media19 04-03-2013 45 1 Download
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Starch-active enzymes often possess starch-binding domains (SBDs) medi-ating attachment to starch granules and other high molecular weight sub-strates. SBDs are divided into nine carbohydrate-binding module (CBM) families, and CBM20 is the earliest-assigned and best characterized family.
24p vinaphone15 25-02-2013 41 2 Download
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Starch synthase III from Arabidopsis thaliana contains an N-terminal region, including three in-tandem starch-binding domains, followed by a C-terminal catalytic domain. We have reported previously that starch-bind-ing domains may be involved in the regulation of starch synthase III func-tion.
13p mobifone23 18-01-2013 42 4 Download
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Starch-binding domains are noncatalytic carbohydrate-binding modules that mediate binding to granular starch. The starch-binding domains from the carbohydrate-binding module family 45 (CBM45, http://www.cazy.org) are found as N-terminal tandem repeats in a small number of enzymes, primarily from photosynthesizing organisms.
11p cosis54 05-01-2013 26 2 Download