Strain selection

The thesis investigates the growth promoting effects of plant growth promoting bacteria (PGPB) in chickpea and wheat. Three main topics covered are: 1) Isolating local Plant Growth Promoting Bacteria; 2) Screening and characterization of isolates; 3) Assessment and identification of the selected strains.

107p runthenight04 02-02-2023 11 4   Download

The present researchaimed to investigate the morphological, biochemical and genotypic characterization of dominant lactic acid bacteria that were isolated from traditional dairy products in Iranian tribes . A total of 75 samples of traditional yogurt, doogh from dairy units in Fars province were randomly collected. Isolation of lactic acid bacteria, biochemical and genotypic identification were conducted. Totally, 157 LAB isolates were selected.

6p mudbound 10-12-2021 31 2   Download

In this study, 341 Bacillus sp. strains were isolated from agricultural soils of Turkey. The potent extracellular lipase producer was selected. It was identified by 16S rRNA, named as Bacillus cereus ATA179. Optimal nutritional and physical parameters for lipase production were determined. Sucrose as the carbon source, (NH4 )2 HPO4 as the nitrogen source, CaCl2 as the metal ion were obtained. The best results of physical parameters were stated at 45°C, pH 7.0, shaking rate 50 rpm, inoculation amount 7% and inoculum age 24 h.

14p thiencuuchu 27-11-2021 19 2   Download

Target: Selecting a yeast strain having high activity of ethanol fermentation producing a specific aroma of fermented pineapple Queen juice, suitable for the production of comprehensive quality brandy from pineapple.

28p thebadguys 08-06-2021 22 3   Download

Research objectives of the thesis: Determining suitable conditions for squalene extraction and purification from selectable S. mangrovei PQ6 strain; evaluating the safety and pharmacological/biological effects of extracted squalene oriented as feed stock for human health food, cosmetics and pharmaceuticals.

27p extraenglish 24-05-2021 22 4   Download

Thesis aims: Isolation and screening for Vietnam antimicrobial marine-derived actinobacteria strains. Isolation and structure elucidation of compounds from 2 promising isolated actinobacteria strains. Determination the antimicrobial activities of isolated compounds.

26p extraenglish 24-05-2021 27 4   Download

Objectives: The aims were to evaluate the effects of some selected endophytic bacteria strains on growth, development of Robusta coffee in a greenhouse and in field conditions. Based on these results, further studies were conducted to determine the effective dose and compatible combination of these strains on the growth and yield of the coffee in the field.

24p prisonbreak123 21-05-2021 18 2   Download

Determining suitable conditions for squalene extraction and purification from selectable S. mangrovei PQ6 strain; evaluating the safety and pharmacological/biological effects of extracted squalene oriented as feed stock for human health food, cosmetics and pharmaceuticals.

27p capheviahe27 23-02-2021 26 4   Download

Isolation and screening for Vietnam antimicrobial marine-derived actinobacteria strains; isolation and structure elucidation of compounds from 2 promising isolated actinobacteria strains; determination the antimicrobial activities of isolated compounds.

26p capheviahe26 25-01-2021 37 5   Download

In this study, we used traditional methods to isolate and screen for fungal laccases that have potential for use in the textile industry (dye decolorization) and a molecular marker to identify studied samples. Fifteen strains of basidiomycetes were isolated and screened for laccase activities on phenol, guaiacol and RBBR. Five strains showed positive laccase activities on all three substrates.

7p tamynhan8 04-11-2020 9 1   Download

Nine Propionibacterium acnes (P. acnes) strains resistant to both clindamycin and erythromycin were selected to investigate the inhibitory ability of extracts and isolated compounds from leaves of Cassia grandis L.f to P. acnes. Ethyl acetate extract inhibited all P. acnes strains, with an aseptic ring diameter of 15-25 mm at a concentration of 200 mg/mL and a MIC value of 30 mg/mL.

11p slimzslimz 08-12-2019 6 2   Download

The research objective of the thesis is to select microorganisms capable of producing laccase, laccaselike from Ba Vi National Forest area, in herbicide / dioxin contaminated soil at Bien Hoa airport. Assessing the ability of decomposing herbicides containing dioxin and reactive dyes by laccase, laccase-like and VSV strains were selected to guide the application in defense activities.

27p xacxuoc4321 11-07-2019 43 5   Download

In this study, we selected a novel gene encoding protease inhibitor from metagenome of marine sponge QT collected in Quang Tri and expressed in the Escherichia coli (E. coli) strain BL21(DE3) for finding and mining novel protease inhibitor.

8p shiwo_ding7 05-06-2019 35 1   Download

We study the enzymatic hydrolysis of lactose by a commercial enzyme from a selected strain of Kluyveromyces fragilis. The variables analyzed were: temperature (25–40 ◦C), enzyme concentration (0.1–3.0 g l−1), lactose concentration (0.0278–0.208 M), and initial galactose concentration (0.0347 M). On the basis of the data analyzed, both published and in the present work, we propose a Michaelis–Menten kinetic model with inhibition by the product (galactose), which reveals that the substrate (lactose) and the product (galactose) present similar affinity for the active site of the enzyme.

10p abk1996 04-09-2018 33 1   Download

An applied field of biology that involves the use of living organisms and bioprocesses in engineering, technology, medicine and other fields requiring bioproducts. (http://en.wikipedia.org/wiki/Biotechnology) It is the products and exploitation from other fundamental sciences.Traditional Biotechnology Traditional biotechnology refers to a number of ancient ways of using living organisms to make new products or modify existing ones. In its broadest definition, traditional biotechnology can be traced back to human's transition from hunter-gatherer to farmer.

29p zingzing09 24-04-2013 63 8   Download

Leaf explants of Echinacea purpurea L. taken from aseptically germinated seedlings were inoculated with A. tumefaciens strains EHA105, carrying a binary vector conferring herbicide resistant bar gene and fungal resistant chitinase gene. Glufosinate ammonium‐resistant shoots were regenerated on a medium containing BAP and NAA at a concentration of 4.88 and 0.053 μM, respectively. A subsequent transfer of shoots to medium containing BAP was necessary for stem elongation and leaf development.

11p zingzing09 24-04-2013 35 2   Download

The herpes simplex virus thymidine kinase⁄ganciclovir (HSV-tk⁄GCV) sys-tem that selectively depletes cells expressing HSV-tk upon treatment with GCV has provided a valuable tool for developing a new animal model expressing the desired tissue damage. In this paper, an HSV-tk vector with an albumin promoter⁄enhancer was constructed. Based on the favourable killing effect on Hep-G2 cells by the recombinant construct, the HSV-tk transgenic mouse strains were developed.

9p awards 06-04-2013 53 3   Download

Three protein factors IF1, IF2 and IF3 are involved in the initiation of translation in prokaryotes. No clear function has been assigned to the smallest of these three factors, IF1. Therefore, to investigate the role of this protein in the initi-ation process inEscherichia coliwe have mutated the cor-responding gene infA. Because IF1 is essential for cell viability and no mutant selection has so far been described, the infA gene in a plasmid was mutated by site-directed mutagenesis in a strain with a chromosomalinfA + gene, followedbydeletionof thisinfA + gene.

11p dell39 03-04-2013 43 5   Download

GTP cyclohydrolase II catalyzes the first dedicated step in the biosynthesis of riboflavin and appears to be a limiting factor for the production of the vitamin by recombinantBacillus subtilisoverproducer strains. Using error-prone PCR amplification, we generated a library of theB. subtilis ribA gene selectively mutated in the GTP cyclohydrolase II domain.

11p vinaphone15 25-02-2013 44 4   Download

Genetic selection has been used to isolate second-site suppressors of a defective cold-sensitive initiation factor I (IF1) R69L mutant ofEscherichia coli. The suppressor mutants specifically map to a single rRNA operon on a plasmid in a strain with all chromosomal rRNA operons deleted.

12p cosis54 05-01-2013 42 5   Download