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Suspension cells
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Ebook "Plant cell culture protocols (Third edition)" is taught around the world as part of undergraduate and graduate courses in agronomical and biological sciences. However, the apparent simplicity of cell culture technology should not lead to mistakingly consider it as trivial. It would be very diffi cult to understand modern plant biotechnology without in vitro cell cultures and even today, the application of recombinant DNA technology to important crops is hampered by the poor embryogenic or morphogenetic response of in vitro cultured tissues.
445p
dongmelo
27-05-2024
5
2
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Ebook "Plant biotechnology for health: From secondary metabolites to molecular farming" emphasis will be put in the relevance of Plant Biotechnology for producing compounds of pharmaceutical and industrial relevance specifically the contribution of in vitro plant cell cultures for producing recombinant proteins (molecular farming) and compounds produced by plants useful for human and animal health (secondary metabolites) will be discussed.
174p
nhanphanguyet
28-01-2024
3
2
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Cultivation technology of Chlorella vulgaris shows great potential in wastewater treatment along with biomass collection for biofuel production. Suspended, autotrophic cultivation at various scales is still the main method to increase algal biomass in different stages of algae technology. In this study, C. vulgaris was cultured to proliferate in a 2 l suspension system to prepare biomass for immobilised cultivation in a biofilm photobioreactor.
15p
vithales
19-04-2022
8
1
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Droplet-based microfluidic devices have become widely used to perform single-cell RNA sequencing (scRNA-seq). However, ambient RNA present in the cell suspension can be aberrantly counted along with a cell’s native mRNA and result in cross-contamination of transcripts between different cell populations.
15p
viarchimedes
26-01-2022
8
0
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IJs released from infected larvae would have the potential to infect healthy mosquito larvae. Also, both bacterial supernatant and bacterial cell suspension of Xenorhabdus nematophila caused >91% larval mortality after 48 h, whereas only the bacterial cell suspension of Photorhabdus laumondii was effective against the mosquito larvae. These data provides useful information on the potential use of EPNs and/or formulated bacterial cell suspensions in the control of the important urban and container-breeding mosquito, Ae.
12p
dolomite36
30-12-2021
7
0
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The effects of UV-B light force, exposure time and incubation period on producing caffeic acid derivatives and growth parameters in Echinacea purpurea callus and suspension culture were assessed. UV-B led to an increment of all growth parameters and antioxidant activity in callus and cell suspension and caffeic acid derivatives in cell suspension by increasing incubation period.
10p
schindler
30-12-2021
10
0
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Mexico is considered the diversification center for chili species, but these crops are susceptible to infection by pathogens such as Colletotrichum spp., which causes anthracnose disease and postharvest decay in general.
13p
vijichea2711
28-05-2021
16
1
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In present study, the optimal conditions for high squalene production of Thraustochytrium sp. TN22 were investigated. Additionally, the methods for extraction and purification of squalene from cell suspension of this strain were developed.
13p
nguaconbaynhay10
20-02-2021
18
2
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In order to set up a system for production of recombinant proteins using suspension cell culture of Nicotiana tabacum cv. Bright Yellow 2 (BY-2), in vitro culture and genetic transformation protocols of such cells were optimised at the laboratory of Plant Cell Biotechnology, Institute of Biotechnology.
10p
tamynhan8
04-11-2020
8
1
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Quantitative phase imaging (QPI) is an established tool for the marker-free classification and quantitative characterization of biological samples. For spherical objects, such as cells in suspension, microgel beads, or liquid droplets, a single QPI image is sufficient to extract the radius and the average refractive index.
8p
vicolorado2711
22-10-2020
11
1
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The tumor-derived GL261 cell line is used as a model for studying glioblastoma and other high-grade gliomas, and can be cultured adherently or as free-floating aggregates known as neurospheres.
7p
vidhaka2711
31-07-2020
11
1
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Genetic uniformity of in vitro raised plants is a prerequisite for production of quality planting material of banana. Embryogenic cultures were induced from immature male flower buds of two important cultivars of banana, ‘Rasthali’ (Silk-Musa AAB) and ‘Grand Naine’ (Cavendish-Musa AAA). Cell suspensions were established and regenerated plants were evaluated for their genetic stability using 10 inter simple sequence repeat markers.
11p
angicungduoc6
20-07-2020
9
1
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Studies have been carried out to determine the effect of activated aqueous solutions (anolytes and catholytes) on multi-resistant strain of Pseudomonas aeruginosa in vitro after storage at room temperature. The solutions were obtained by electrochemical activation of water with 0.8% NaCl, and with a combination of 0.4% NaCl and 0.4% Na2CO3. The disinfectant Virkon S was used as a control. Cultures were made on Cetrimide agar after different intervals of action of the solutions on P. aeruginosa suspensions (106 cells/ml).
8p
angicungduoc5
14-06-2020
9
0
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Hepatitis B virus (HBV) is a DNA virus belonging to the Hepadnaviridae family that infect humans causing a disease infecting liver cells displaying a substantial hepatic tissue tropism, and inducing chronic liver damage and subsequent Hepato carcinogenesis. In Oncology patients receive packed red blood cell suspensions and other blood products regularly and they are in the high-risk group for infections due to these viruses.
6p
angicungduoc5
12-06-2020
18
1
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Banana (Musa spp.) constitute a hybrid-polyploid complex and are classified according to different genome compositions such as AA, BB, AB, AAA, AAB, ABB, AAAA, ABBB, AAAB, AABB and AAABB. Knowledge of ploidy and exact genome compositions of the parental material is essential for Musa breeding. Flow cytometric analysis of nuclear DNA content was used to estimate ploidy levels.
14p
trinhthamhodang1213
27-05-2020
15
0
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Senescence is a key developmental process occurring during the life cycle of plants that can be induced also by environmental conditions, such as starvation and/or darkness.
11p
vihashirama2711
21-05-2020
7
1
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The kinetics of primary and secondary immune response of Indian major carps, Catla catla, Labeo rohita and Cirrhinus mrigala to sheep red blood cells (SRBC)were studied in detail at three different age groups of 120,150 and 270 days old. Fish were immunized by injection (i.m) with 106 cells/fish of SRBC suspension. The second injection was given at 20 days after the first injection with same dose. All age groups of each species produced detectable humoral antibody titers on 6th days after the first injection.
6p
nguaconbaynhay5
16-05-2020
14
0
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Neem tree serves as a cornucopia for triterpenoids called limonoids that are of profound interest to humans due to their diverse biological activities. However, the biosynthetic pathway that plant employs for the production of limonoids remains unexplored for this wonder tree.
21p
vishikamaru2711
29-04-2020
16
1
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Thaxtomin A (TA) is a natural cellulose biosynthesis inhibitor (CBI) synthesized by the potato common scab-causing pathogen Streptomyces scabies. Inhibition of cellulose synthesis by TA compromises cell wall organization and integrity, leading to the induction of an atypical program of cell death (PCD)
13p
viharuno2711
21-04-2020
10
0
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High-yield production of human Dicer by transfection of human HEK293-EBNA1 cells grown in suspension
Dicer is a 219-kDa protein that plays key roles in gene regulation, particularly as the ribonuclease III enzyme responsible for cleaving precursor miRNA substrates. Its enzymatic activity is highly regulated by protein factors, and this regulation can impact on the levels of miRNAs and modulate the behavior of a cell.
13p
vihamax2711
21-04-2020
18
0
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