Thermococcus

We have previously identi®ed a DNA ligase (LigTk)froma hyperthermophilic archaeon,Thermococcus kodakaraensis KOD1. The enzyme is the only characterized ATP-depend-ent DNA ligase from a hyperthermophile, and allows the analysis of enzymatic DNA ligation reactions at tempera-tures above themeltingpoint of the substrates.Herewe have focused on the interactions of LigTkwith various DNA substrates, and its speci®cities toward metal cations.

7p research12 23-04-2013 31 2   Download

Fifty-nine amino acid sequences belonging to family 57 (GH-57) of the glycoside hydrolases were collected using the CAZy server, Pfamdatabase andBLASTtools. Owing to the sequence heterogeneity of the GH-57 members, sequence alignments were performed using mainly manual methods. Likewise, five conserved regions were identified, which are postulated to be GH-57 consensus motifs.

10p dell39 03-04-2013 32 2   Download

Conformational studies on amyloidb peptide (Ab) in aqueous solution are complicated by its tendency to aggregate. In this study, we determined the atomic-level structure of Ab28)42 in an aqueous environment. We fused fragments of Ab, residues 10–24 (Ab10)24) or 28–42 (Ab28)42), to three positions in the C-terminal region of ribonuclease HII from a hyper-thermophile, Thermococcus kodakaraensis(Tk-RNase HII).

9p dell39 27-03-2013 36 3   Download

The crystal structure of glycerol kinase from the hyperthermophilic archa-eon Thermococcus kodakaraensis(Tk-GK) in a dimeric form was deter-mined at a resolution of 2.4 A˚ . This is the first crystal structure of a hyperthermophilic glycerol kinase.

12p media19 06-03-2013 39 3   Download

Thế giới chúng ta đang bị phụ thuộc nặng nề vào một nền kinh tế nhiên liệu hóa thạch. Nhiên liệu sử dụng cho các phương tiện giao thông hiện tại như: xe hơi, xe lửa, máy bay… là từ dầu mỏ. Hơn nữa, một tỉ lệ khá cao các nhà máy điện là nhiệt điện dùng nhiên liệu dầu, khí thiên nhiên hay than đá. Nếu không có nhiên liệu hóa thạch,

27p pinyeu_dangsac 26-01-2013 155 42   Download

Pro-Tk-SP fromThermococcus kodakaraensisconsists of the four domains: N-propeptide, subtilisin (EC 3.4.21.62) domain,b-jelly roll domain and C-propeptide. To analyze the maturation process of this protein, the Pro-Tk-SP derivative with the mutation of the active-site serine residue to Cys (Pro-Tk-S359C), Pro-Tk-S359C derivatives lacking the N-propeptide (ProC-Tk-S359C) and both propeptides (Tk-S359C), and a His-tagged form of the isolated C-propeptide (ProC*) were constructed.

11p cosis54 05-01-2013 37 4   Download

Tuyển tập các báo cáo nghiên cứu về y học được đăng trên tạp chí y học Minireview cung cấp cho các bạn kiến thức về ngành y đề tài: Genome analysis and genome-wide proteomics of Thermococcus gammatolerans, the most radioresistant organism...

0p thulanh21 12-11-2011 47 2   Download