Tissue culture method

Part 2 book "Color atlas of diagnostic microbiology" includes content: Mycobacteria, microbial pahogens isolated and/or inentified by tissue culture of other special methods, antimicrobial susceptibility testing, molecular techniques.

122p zizaybay1101 30-04-2024 6 2   Download

Part 1 book "Animal cell culture - Essential methods" includes content: The cell culture laboratory, sterilization, microscopy of living cells, basic techniques and media, the maintenance of cell lines, and safety, development and optimization of serum and protein free culture media.

214p oursky04 14-09-2023 5 3   Download

Part 2 book "Animal cell culture - Essential methods" includes content: Cryopreservation and banking of cell lines, primary culture of specific cell types and the establishment of cell lines, cloning, the quality control of animal cell lines and the prevention, detection and cure of contamination, systems for cell culture scale up, good laboratory practice in the cell culture laboratory.

166p oursky04 14-09-2023 5 3   Download

Seed-borne rice endophytes are capable of disseminating into host plant tissues as well as to their rhizosphere. Here, we investigated the occurrence of siderophore-producing bacteria (SPB) in the seed endospheres of two distinct rice (Oryza sativa L.) cultivars, TK8 (ssp. japonica) and TCN1 (ssp. indica), and their dissemination into the rhizospheres through culture-dependent methods.

13p viengels 25-08-2023 6 2   Download

Part 1 of ebook "Plant biotechnology: Principles and applications" provide readers with content about: historical perspective and basic principles of plant tissue culture; plant tissue culture - applications in plant improvement and conservation; plant genetic resources - their conservation and utility for plant improvement;... Please refer to the part 1 of ebook for details!

204p langmongnhu 14-12-2022 10 3   Download

Tissue culture is an effective method for the rapid breeding of seedlings and improving production efficiency, but explant browning is a key limiting factor of walnut tissue culture. Specifically, the polymerization of PPO-derived quinones that cause explant browning of walnut is not well understood.

12p vijichea2711 28-05-2021 12 1   Download

The in vitro propagation method can be used for clonal propagation of selected germplasm, genetic improvement, production of active compound in cell culture. In vitro propagation in C. wightii has been attempted through organogenesis and somatic embryogenesis methods by various researchers.

7p cothumenhmong11 11-05-2021 12 2   Download

The present study demonstrates a simple and efficient method for callus induction of Bamboo. The leaf, node and seed are used as explants for induction of callus. These explants were subjected to sterilization treatment and it is found that Tween -20, bavistin and ethanol are sufficient for proper sterilization of explants. Then these explants were subjected to various concentrations of growth hormone 2,4-Dichlorophenoxyacetic acid (2,4-D) added in Murashige and Skoog Media. It was concluded that the seeds are suitable explants for rapid induction of callus.

10p chauchaungayxua10 18-03-2021 7 1   Download

The use of plant tissue culture as a routine method of potato seed production would be costly but these techniques can be used to eliminate the pathogens, produce required initial material and then, use another efficient and cheaper system to rapidly produce high quality seed tubers for commercial production. Considering this, the liquid formulation of antimycotic 100x, and amphotericin-B 250 µg/ml of PAA make was used.

8p cothumenhmong9 18-01-2021 13 2   Download

The yield potential as well as quality of chilli is affected by a number of biotic and abiotic stresses. The genetic improvement of this crop, especially for characters like resistance to biotic and abiotic stresses using conventional breeding methods are laborious, time taking and also needs a large experimental area. So, an attempt was made to study callus induction in chilli genotypes by using hypocotyls as explant to complement conventional breeding and expedite capsicum improvement.

6p cothumenhmong9 18-01-2021 12 2   Download

The purpose of this meta-analysis was to evaluate the diagnostic accuracy of periprosthetic tissue culture in blood culture bottles (BCB) for periprosthetic joint infection (PJI). Methods: PubMed, Web of Science, and Embase were systematically searched for eligible studies evaluating the diagnostic performance of periprosthetic tissue culture in BCB for the diagnosis of PJI.

8p vivermont2711 28-01-2021 12 2   Download

Recently reported data have suggested that only a small subset of cancer cells possess the capability to initiate malignancies. These observations were based on investigation of cells within the primary tumors displaying a distinct surface marker pattern. CD133 marker is a putative hematopoietic and neuronal stem cell marker, which is also considered to be a tumorigenic marker in brain, prostate and liver. Recent studies have shown that a small population of CD133-positive cells, indeed, exists in human hepatocellular carcinoma (HCC) cell lines and primary HCC tissues.

11p trinhthamhodang9 10-12-2020 24 1   Download

Stevia rebaudiana is herbaceous perennial plant of Asteraceae family and an excellent substitute of sugar with medicinal importance. The potential uses of Stevia which produces stevioside, a non-caloric sweetener that does not metabolize in the human body, hence control blood sugar level. Conventional propagation methods are not produce adequate planting material. Plant tissue culture techniques are only technique to produce quality planting material.

13p chauchaungayxua8 03-10-2020 9 1   Download

Cucurbitaceae is the one of the elite family in the plant kingdom and have importance in its daily utilization for cuisine preparation as a source of vegetable and medicinal plant. This family consists of hundreds of edible species, qualitative and quantitative improvement plays a vital role in the processing industry and Indian medicine system of Ayurveda (AYUSH). Plant tissue culture techniques have been used extensively for propagation of cucurbitaceae by using various explants and methods from last few decades.

24p trinhthamhodang1215 23-09-2020 10 1   Download

The high incidence of breast cancer has sparked the development of novel targeted and personalized therapies. Personalization of cancer treatment requires reliable prediction of chemotherapy responses in individual patients.

13p vinaypyidaw2711 26-08-2020 9 2   Download

Pomegranate can be propagated by seed, air layering, grafting, shoot cuttings and tissue culture. Seed propagation is used to raise the rootstocks that can be utilized for germplasm conservation and rootstocks breeding purpose. Vegetative propagation methods are used to multiply selected genotypes that bring similar character in form of clones having similar characters to mother plant. Vegetative propagation techniques (hard wood cutting, layering) are practices to raise the saplings for commercial production in pomegranate, because it perpetuates all characteristics of each cultivar.

16p nguaconbaynhay7 15-08-2020 16 1   Download

In light of the same, the objectives of this study include to isolate various Staphylococcus species from clinical specimens. And also to compare three phenotypic methods for detection of biofilm formation among the isolated Staphylococcus species, namely, Tube adherence method, Tissue culture plate method and Congo red agar method.

9p nguaconbaynhay7 15-08-2020 13 1   Download

Plant tissue culture is the method of culturing the plant cells or tissues under controlled aseptic conditions on a solid or liquid medium. It has value in basic research for the production of true to type, disease free and micro-propagated plants. Besides this also has commercial application. This review work outlines the work done on pertaining to various aspects of in vitro micro-propagation, regeneration, rooting, hardening, in potato.

7p kethamoi6 29-06-2020 24 1   Download

The present study is on the isolation, characterization and screening of endophytic bacterium i.e., Gluconacetobacter diazotrophicus which was isolated from the tissues of surface sterilized roots of four different crops viz., sugarcane, maize, pineapple and carrot. The morphological and biochemical tests permitted characterization of Gluconacetobacter diazotrophicus isolates.

10p chauchaungayxua6 26-06-2020 17 1   Download

A survey was conducted in some districts of Upper Brahmaputra Valley Zone of Assam for most prevailing diseases in brinjal during the year 2015-16. Apart from the fruit rot disease of brinjal (Solanum melongena L.) caused by Phomopsis, Phytophthora, Alternaria etc., a new pathogen, Phoma exigua was observed. Initial symptom developed on the fruits as minute, slightly sunken spots, which later produced minute black bodies (pycnidia) scattered and immersed in the infected host tissues.

4p gaocaolon5 14-06-2020 6 1   Download