Tissue culture

Nuôi cấy mô, tế bào thực vật ( plant tissue culture) là kỹ thuật đưa một mô, bộ phận hoặc tế bào của thực vật vào trong một hệ thống vô trùng có thể kiểm soát về: thành phần chất khoáng, điều hoà sinh trưởng, các chất hữu cơ cung cấp cho cây.

22p ngovanquang12c3 18-12-2010 1062 265   Download

Eucalyptus is one of the important plant species for paper pulp, fuel wood, and timber. In this study, a hybrid Eucalyptus, U16 line, was investigated for rapid in vitro propagation. The apical and adventitious shoots served as initial explants.

8p viprimi 16-12-2024 1 1   Download

To identify species, biological traits, chemical composition, and bioactive activities of essential oil and to develop propagation technical process using tissue culture techniques from shoot bud cutting to contribute to the conservation and development of genetic sources of native ginger plants in Bac Kan.

27p trinhthamhodang7 31-08-2020 46 2   Download

Connective tissue growth factor/hypertrophic chondrocyte specific gene product 24 (CTGF/Hcs24) promotes proliferation and differentiation of chondrocytes in culture. We investigated the roles of two major types of mitogen activated protein kinase (MAPK) in the promotion of proliferation and differentiation by CTGF/Hcs24. Here we report the effects of the MAPKK/MEK 1/2 inhibitor, PD098059, and p38 MAPK inhibitor, SB203580, in a human chondrosarcoma-derived chondrocytic cell line (HCS-2/8) and rabbit growth cartilage (RGC) cells treated with CTGF/ Hcs24....

8p system191 01-06-2013 42 3   Download

Duodenase is a 29-kDa serine endopeptidase that displays selective trypsin- and chymotrypsin-like substrate specificity. This enzyme has been localized to epitheliocytes of Brunner’s glands, and as described here, to mast cells within the intestinal mucosa and lungworm-infected lung, implying an important additional role in inflammation and tissue remodelling. In primary cultures of pulmonary artery fibroblasts, duodenase induced a concentration-dependent increase in [3H]thymidine incorporation with a maximal effect observed at 30 nM.

10p research12 01-06-2013 54 3   Download

Matrilysin (matrix metalloproteinase-7) plays important roles in tumor progression. It was previously found that matrilysin binds to the surface of colon cancer cells to promote their metastatic potential. In this study, we identified annexin II as a novel membrane-bound substrate of matrilysin. Treatment of human colon cancer cell lines with active matrilysin released a 35 kDa annexin II form, which lacked its N-terminal region, into the culture supernatant. The release of the 35 kDa annexin II by matrilysin was significantly enhanced in the presence of serotonin or heparin....

14p research12 29-04-2013 41 2   Download

The lipopolysaccharide (LPS) ofChlamydia trachomatis serotype E was isolated from tissue culture-grown element-ary bodies and analyzed structurally by mass spectrometry and 1 H, 13 Cand31 P nuclear magnetic resonance. The LPS is composed of the same pentasaccharide bisphosphate aKdo-(2–8)-aKdo-(2–4)-aKdo-(2–6)-bGlcN-4P-(1–6)-aGlcN-1P (Kdo is 3-deoxy-a-D-manno-oct-2-ulosonic acid) as reported forC. trachomatisserotype L2[Rund,S.,Lind-ner,B.,Brade,H. and Holst,O. (1999)J. Biol. Chem.274, 16819–16824].

11p tumor12 20-04-2013 39 4   Download

This work investigated the kinetics of catabolism and the catabolic fate of the newly synthesized 35 S-labelled proteo-glycans present in explant cultures of tendon. Tissue from the proximal region of bovine deep flexor tendon was incu-bated with [ 35 S]sulfate for 6 h and then placed in explant cultures for periods of up to 15 days. The amount of radi-olabel associatedwithproteoglycans and free [ 35 S]sulfate lost to themediumand retained in thematrixwas determined for each day in culture.

9p dell39 03-04-2013 22 3   Download

The cellular form of prion protein (PrP C ) is anchored to the plasma mem-brane of the cell and expressed in most tissues, but predominantly in the brain, including in the pituitary gland. Thus far, the biosynthesis of PrP C has been studied only in cultured (transfected) tumour cell lines and not in primary cells.

16p dell39 27-03-2013 31 2   Download

We applied metabolic control analysis to the Kennedy pathway for triacyl-glycerol formation in tissue cultures from the important oil crops, olive (Olea europaeaL.) and oil palm (Elaeis guineensisJacq.). When microsomal fractions were incubated at 30
C rather than 20
C, there was an increase in triacylglycerol labelling.

7p dell39 27-03-2013 50 4   Download

During the past decade or so, a wealth of information about metabolites in various human brain tumour preparations (cultured cells, tissue specimens, tumours in vivo) has been accumulated by global profiling tools. Such hol-istic approaches to cellular biochemistry have been termed metabolomics.

8p galaxyss3 21-03-2013 43 2   Download

Acidic extracellular pH is a common feature of tumor tissues. We have reported that culturing cells at acidic pH (5.4–6.5) induced matrix metallo-proteinase-9 expression through phospholipase D, extracellular signal regu-lated kinase 1⁄2 and p38 mitogen-activated protein kinases and nuclear factor-jB.

13p galaxyss3 19-03-2013 33 5   Download

Detailed understanding of the dynamics of living systems requires a means to monitor, in real time, changes in the levels of key components in response to specific stimuli. Applications involving cultured cells, tissue slices and implantation in living systems are discussed.

10p media19 04-03-2013 20 1   Download

Tuyển tập báo cáo các nghiên cứu khoa học quốc tế ngành y học dành cho các bạn tham khảo đề tài: Quantification of the tissue-culture induced variation in barley (Hordeum vulgare L.)

9p panasonic06 26-12-2011 54 4   Download

Tuyển tập báo cáo các nghiên cứu khoa học quốc tế ngành y học dành cho các bạn tham khảo đề tài: Tissue culture-induced transpositional activity of mPing is correlated with cytosine methylation in rice

15p panasonic06 25-12-2011 43 3   Download

Tuyển tập báo cáo các nghiên cứu khoa học quốc tế ngành y học dành cho các bạn tham khảo đề tài: Prospects of micromass culture technology in tissue engineering

4p panasonic05 22-12-2011 34 4   Download

Tuyển tập báo cáo các nghiên cứu khoa học quốc tế ngành y học dành cho các bạn tham khảo đề tài: The inflammatory response seen when human omental adipose tissue explants are incubated in primary culture is not dependent upon albumin and is primarily in the nonfat cells...

9p thulanh27 13-12-2011 39 4   Download

Tuyển tập các báo cáo nghiên cứu về y học được đăng trên tạp chí y học General Psychiatry cung cấp cho các bạn kiến thức về ngành y đề tài: Tumor necrosis factor and norepinephrine lower the levels of human neutrophil peptides 1-3 secretion by mixed synovial tissue cultures in osteoarthritis and rheumatoid arthritis...

9p thulanh16 21-10-2011 41 3   Download

Tuyển tập các báo cáo nghiên cứu về lâm nghiệp được đăng trên tạp chí lâm nghiệp quốc tế đề tài: Vegetative propagation of oak (Quercus robur and Q petraea) by cutting and tissue culture...

13p toshiba7 08-10-2011 78 2   Download

Proceedings of scientific reports of an agricultural college topics: farm workers to lead the four strains by culturing techniques mo.Theo policy of the Ministry of Agriculture and Rural Development to the year 201o acreage Cayenne example only of Vietnam ..

6p thulanh2 07-09-2011 75 4   Download