Types of hybridization

Concept of hybrid molecules: When double strand DNA is steamed to a temperature exceed the melting temperature (Tm), it will separate into 2 single strands DNA due to breaks of H bonds. If the reaction temperature is then decreased slowly plus other appropriate experimental conditions, these ssDNA will pair again. This phenomenon is called the hybridization of molecules. Characteristic of the hybrid molecules: Specificity, the re-pairing occurs only between two complementary sequences.

71p ngovanquang12c3 17-12-2010 120 27   Download

This research work first aims to develop a microgrid architecture integrating dispatchable and non-dispatchable distributed generation units in a series-cascaded manner. Existing control strategies for cascaded microgrids focus on dispatchable type generation only. However, adequate power sharing and voltage regulation of a microgrid containing mixed dispatchable and non-dispatchable cascaded generation units demand new control approaches to achieve operational performance and reliability comparable to the conventional parallel-topology microgrid.

186p runthenight04 02-02-2023 7 1   Download

We have determined the nucleotide sequence of a zebrafish cDNAclone that codes for a cellular retinol-binding protein type II (CRBPII). Radiation hybrid mapping revealed that the zebrafish and human CRBPII genes are located in syntenic groups.In situhybridization and emulsion autora-diography localized theCRBPIImRNA to the intestine and the liver of adult zebrafish.

8p research12 23-04-2013 45 4   Download

The low affinity of peptide nucleic acid (PNA) to hybridize with DNA in the presence of a mismatch endows PNA with a high degree of discriminat-ory capacity that has been exploited in therapeutics for the selective inhibi-tion of the expression of point-mutated genes. To obtain a structural basis for this intriguing property, molecular dynamics simulations are carried out on PNAÆDNA duplexes formed at the Ki-ras proto-oncogene, compri-sing the point-mutated (GAT), and the corresponding wild-type (GGT) codon 12. ...

16p fptmusic 11-04-2013 53 2   Download

The Na + F1F0 ATP synthase operon of the anaerobic, acetogenic bacte-rium Acetobacterium woodiiis unique because it encodes two types of csubunits, two identical 8 kDa bacterial F0-like csubunits (c2 and c3 ), with two transmembrane helices, and a 18 kDa eukaryal V0 -like (c1 ) csubunit, with four transmembrane helices but only one binding site.

9p media19 06-03-2013 36 2   Download

Ribonucleases H are enzymes that cleave the RNA of RNA⁄DNA hybrids that form during replication and repair and which could lead to DNA instability if they were not processed. There are two main types of RNase H, and at least one of them is present in most organisms.

12p vinaphone15 27-02-2013 27 3   Download

Tuyển tập báo cáo các nghiên cứu khoa học quốc tế ngành hóa học dành cho các bạn yêu hóa học tham khảo đề tài: Research Article Common Fixed Point Theorems for Hybrid Pairs of Occasionally Weakly Compatible Mappings Satisfying Generalized Contractive Condition of Integral Type

9p sting10 07-03-2012 43 3   Download

Akt regulates various cellular processes, including cell growth, survival, and metabolism. Recently, Akt's role in neurite outgrowth has also emerged. We thus aimed to identify neuronal function-related genes that are regulated by Akt. Methods: We performed suppression subtractive hybridization on two previously established PC12 sublines, one of which overexpresses the wild-type (WT) form and the other, the dominant-negative (DN) form of Akt. These sublines respond differently to NGF's neuronal differentiation effect. Results: A...

11p toshiba23 18-11-2011 51 4   Download

Tuyển tập các báo cáo nghiên cứu về lâm nghiệp được đăng trên tạp chí lâm nghiệp quốc tế đề tài:"Effect of article genotype and cutting type on the vegetative propagation of the pine hybrid (Pinus brutia (Ten) x Pinus halepensis (Mill)...

8p toshiba7 08-10-2011 54 2   Download