Yeast strain

Target: Selecting a yeast strain having high activity of ethanol fermentation producing a specific aroma of fermented pineapple Queen juice, suitable for the production of comprehensive quality brandy from pineapple.

28p thebadguys 08-06-2021 22 3   Download

Deproteinated cheese whey was used for biomass production of probiotic strain Lactobacillus helveticus MTCC 5463 in a biofermenter. Optimization of growth parameters such as temperature, pH and time of incubation as well as nutrient supplementation of cheese whey was carried out using response surface methodology (RSM). Cheese whey supplemented with 0.95% yeast extract and 0.95% proteose peptone, inoculated with 6% (v/v) active culture of L. helveticus MTCC 5463 and fermented for 24 h at optimized temperature of 40ºC and pH 6.25 yielded 3.25 g/L dry cell biomass and 14.

14p chauchaungayxua6 26-06-2020 27 3   Download

Mitochondrial ribosomal proteins (mrps) of the budding yeast, Saccharomyces cerevisiae, have been extensively characterized genetically and biochemically. However, the list of thegenes encoding individualmrps is still not complete and quite a fewof the mrps are only predicted from their similarity to bacterial ribosomal proteins. We have con-structed a yeast strain in which one of the small subunit proteins, termed Mrp4, was tagged with S-peptide and used for affinity purification of mitochondrial ribosome. ...

12p tumor12 22-04-2013 52 2   Download

In eukaryotes, pre-rRNA processing depends on a large number of non-ribosomal trans-acting factors that form large and intriguingly organized complexes. A novel nucleolar protein, Nop53p, was isolated by using Nop17p as bait in the yeast two-hybrid system. Nop53p also interacts with a second nucleolar protein, Nip7p. A carbon source-conditional strain with the NOP53coding sequence under the control of theGAL1promoter did not grow in glucose-containing medium, showing the phenotype of an essential gene. ...

14p fptmusic 11-04-2013 32 2   Download

The addition of glucose toSaccharomyces cerevisiaecells causes reprogramming of gene expression. Glucose is sensed bymembrane receptorsaswell as (so far elusive) intracellular sensing mechanisms. The availability of four yeast strains that display different hexose uptake capacities allowed us to study glucose-induced effects at different glycolytic rates. Rapid glucose responses were observed in all strains able to take up glucose, consistent with intracellular sensing.

10p awards 05-04-2013 33 4   Download

During a search for cDNAs encoding plant sterol acyl-transferases, we isolated four full-length cDNAs fromAra-bidopsis thaliana that encode proteins with substantial identity with animal lecithin : cholesterol acyltransferases (LCATs). The expressionof one of these cDNAs,AtLCAT3 (At3g03310), invarious yeast strains resulted in the doubling of the triacylglycerol content.

13p awards 05-04-2013 39 4   Download

A fatty-acid-metabolizing enzyme fromArabidopsis thaliana, CYP94C1, belonging to the cytochrome P450 family was cloned and characterized. CYP94C1 was heterologously expressed in aSaccharomyces cerevisiae strain (WAT11) engineered for P450 expression. When recombinant yeast microsomes were incubated with lauric acid (C12:0) for 15 min, one major metabolite was formed.

12p media19 04-03-2013 42 2   Download

The assembly status of the cytochromebc1 complex has been analyzed in distinct yeast deletion strains in which genes for one or more of thebc1 subunits were deleted. In all the yeast strains tested, a bc1 sub-complex of approximately 500 kDa was found when the mitochondrial membranes were analyzed by blue native electrophoresis.

15p viettel02 22-02-2013 32 3   Download

Tuyển tập các báo cáo nghiên cứu về y học được đăng trên tạp chí y học Wertheim cung cấp cho các bạn kiến thức về ngành y đề tài: Global fitness profiling of fission yeast deletion strains by barcode sequencing...

0p thulanh22 20-11-2011 35 2   Download

Studies of mammalian prion diseases such as bovine spongiform encephalopathy have suggested that different strains consist of prion proteins with different conformations. Two recent studies of yeast prions have now formally demonstrated that multiple stable protein conformations are the basis of strain variation. deposited research Transmissible spongiform encephalopathies (TSEs) are a group of closely related neurodegenerative conditions of animals

0p thulanh21 15-11-2011 71 1   Download

Tạp chí KHKT Nông nghiệp, Tập 1, số 3/2003 ?Decalactone của chủng nấm men Yarrowia lipolytica W29 Several facrors affecting biosynthesis of gama- decalactone by yeast strain Yarrowia lipolytica W29 Lại Thị Ngọc Hà1 summary A study was undertaken to determine factors affecting biosynthesis of gama-decalactone by yeast strain Yarrowia lipolytica W29. It was shown that Yarrowia lipolytica W29 best produced gama-decalactone under the following conditions: concentration of ricin oil 2g/200ml, culture pH of 7, and agitation speed of 200 rounds/minut.

6p thulanh2 07-09-2011 73 8   Download

Problem statement: Dilute sulphuric acid and enzymatic hydrolysis methods were used for sugar extraction. Xylose and glucose sugars were obtained from corn cobs. Approach: Acid hydrolysis of corn cobs gave higher amount of sugars than enzymatic hydrolysis. Results: The results showed that optimal temperature and time for sugar fermentation were approximately 25°C and 50 h by two yeast strains (S. cerevisiae and P. Stipitis) respectively.

6p nguyenngocsonctu 30-11-2010 140 17   Download