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Accurate modelling of the retention behaviour of peptides in gradient-elution hydrophilic interaction liquid chromatography
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The applicability of models to describe peptide retention in hydrophilic interaction liquid chromatography (HILIC) was investigated. A tryptic digest of bovine-serum-albumin (BSA) was used as a test sample. Several different models were considered, including adsorption, mixed-mode, exponential, quadratic and Neue–Kuss models.
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