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Báo cáo khoa học: A designed curved DNA segment that is a remarkable activator of eukaryotic transcription

Chia sẻ: Nguyen Thang | Ngày: | Loại File: PDF | Số trang:12

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To identify artificial DNA segments that can stably express transgenes in the genome of host cells, we built a series of curved DNA segments that mimic a left-handed superhelical structure. Curved DNA segments of 288 bp (T32) and 180 bp (T20) were able to activate transcription from the herpes simplex virus thymidine kinase (tk) promoter by approximately 150-fold and 70-fold, respectively, compared to a control in a transient transfection assay in COS-7 cells.

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Nội dung Text: Báo cáo khoa học: A designed curved DNA segment that is a remarkable activator of eukaryotic transcription

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