intTypePromotion=1
zunia.vn Tuyển sinh 2024 dành cho Gen-Z zunia.vn zunia.vn
ADSENSE

Báo cáo khoa học: A dimer of the FeS cluster biosynthesis protein IscA from cyanobacteria binds a [2Fe2S] cluster between two protomers and transfers it to [2Fe2S] and [4Fe4S] apo proteins

Chia sẻ: Nguyen Thang | Ngày: | Loại File: PDF | Số trang:10

35
lượt xem
2
download
 
  Download Vui lòng tải xuống để xem tài liệu đầy đủ

Two proteins with similarity to IscA are encoded in the genome of the cyanobacteriumSynechocystisPCC 6803. One of them, the product ofslr1417 which accounts for 0.025% of the total soluble protein ofSynechocystiswas over-expressed inE. coliand purified.The purified protein was found to be mainly dimeric and did not contain any cofactor.Incubation with iron ions, cysteine andSynecho-cystisIscS led to the formation of one [2Fe2S] cluster at an IscA dimer as demonstrated (by the binding of about one iron and one sulfide ion per IscA monomer) by UV/Vis, EPR and Mo¨ssbauer spectroscopy....

Chủ đề:
Lưu

Nội dung Text: Báo cáo khoa học: A dimer of the FeS cluster biosynthesis protein IscA from cyanobacteria binds a [2Fe2S] cluster between two protomers and transfers it to [2Fe2S] and [4Fe4S] apo proteins

ADSENSE

CÓ THỂ BẠN MUỐN DOWNLOAD

 

Đồng bộ tài khoản
2=>2