Báo cáo khoa học: An alternative pathway of vitamin D2metabolism Cytochrome P450scc (CYP11A1)-mediated conversion to 20-hydroxyvitamin D2and 17,20-dihydroxyvitamin D2
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We report an alternative, hydroxylating pathway for the metabolism of vitamin D2 in a cytochrome P450 side chain cleavage (P450scc; CYP11A1) reconstituted system. NMR analyses identified solely 20-hydroxyvitamin D2and 17,20-dihydroxyvitamin D2derivatives. 20-Hydroxyvitamin D2was produced at a rate of 0.34 molÆmin )1 Æmol )1 P450scc, and 17,20-dihydroxy-vitamin D2 was produced at a rate of 0.13 molÆmin )1 Æmol )1 .
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