Báo cáo khoa học: Analysis of the in planta antiviral activity of elderberry ribosome-inactivating proteins
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Although the type-2 ribosome-inactivatingproteins (SNA-I, SNA-V, SNLRP) from elderberry (Sambucus nigraL.) are all devoid of rRNAN-glycosylase activity towards plant ribosomes, some of them clearly show polynucleotide– adenosine glycosylase activity towards tobaccomosaic virus RNA. This particular substrate specificity was exploited to further unravel the mechanism underlying the in planta antiviral activity of ribosome-inactivating proteins. Trans-genic tobacco (Nicotiana tabacumL. cv SamsunNN) plants expressing the elderberry ribosome-inactivating proteins were generated and challengedwith tobaccomosaic virus in order to analyze their antiviral properties. ...
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