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Báo cáo khoa học: Application of the Fc fusion format to generate tag-free bi-specific diabodies

Chia sẻ: Nguyen Thang | Ngày: | Loại File: PDF | Số trang:11

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We previously reported the use of a humanized bi-specific diabody that targets epidermal growth factor receptor and CD3 (hEx3-Db) for cancer immunotherapy. Bacterial expression can be used to express small recombi-nant antibodies on a large scale; however, their overexpression often results in the formation of insoluble aggregates, and in most cases artificial affinity peptide tags need to be fused to the antibodies for purification by affinity chromatography.

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Nội dung Text: Báo cáo khoa học: Application of the Fc fusion format to generate tag-free bi-specific diabodies

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