Báo cáo khoa học: Aspergillus nidulans a-galactosidase of glycoside hydrolase family 36 catalyses the formation of a-galacto-oligosaccharides by transglycosylation

Thea-galactosidase from Aspergillus nidulans(AglC) belongs to a phyloge-netic cluster containing eukaryotica-galactosidases and a-galacto-oligosac-charide synthases of glycoside hydrolase family 36 (GH36). The recombinant AglC, produced in high yield (0.65 gÆL )1 culture) as His-tag fusion in Escherichia coli, catalysed efficient transglycosylation with a-(1fi6) regioselectivity from 40 mm4-nitrophenola-d-galactopyranoside