Báo cáo khoa học: Characterization of the secreted chorismate mutase from the pathogen Mycobacterium tuberculosis

Chia sẻ: Nguyen Thang | Ngày: | Loại File: PDF | Số trang:15

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The gene encompassing ORF Rv1885c with weak sequence similarity to AroQ chorismate mutases (CMs) was cloned from the genome ofMycobac-terium tuberculosis and expressed in Escherichia coli. The gene product (*MtCM) complements a CM-deficient E. colistrain, but only if produced without the predicted N-terminal signal sequence typical ofM. tuberculosis. The mature *MtCM, which was purified by exploiting its resistance to irre-versible thermal denaturation, possesses high CM activity in vitro. The enzyme follows simple Michaelis–Menten kinetics, having a kcat of 50 s )1 and aKmof 180lm(at 30 C and pH 7.5)....

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