Báo cáo khoa học: Critical molecular regions for elicitation of the sweetness of the sweet-tasting protein, thaumatin I
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Thaumatin is an intensely sweet-tasting protein. To identify the critical amino acid residue(s) responsible for elicitation of the sweetness of thau-matin, we prepared mutant thaumatin proteins, usingPichia pastoris,in which alanine residues were substituted for lysine or arginine residues, and the sweetness of each mutant protein was evaluated by sensory analysis in humans.
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