
Báo cáo khoa học: Deflavination and reconstitution of flavoproteins Tackling fold and function
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Flavoproteins are ubiquitous redox proteins that are involved in many biological processes.In the majority of flavoproteins, the flavin cofactor is tightlybut noncovalently bound.Reversible dissociation of flavoproteins into apo-proteinandflavinprostheticgroupyields valuable insights in flavoprotein folding, functionandmechanism.Replacement of the natural cofactorwithartificial flavins has proved tobe especially useful for the determination of the solvent acces-sibility, polarity, reaction stereochemistry and dynamic behaviour of flavoprotein active sites....
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