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Báo cáo khoa học: Development of a baculovirus-based fluorescence resonance energy transfer assay for measuring protein–protein interaction

Chia sẻ: Nguyen Thang | Ngày: | Loại File: PDF | Số trang:9

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A new baculovirus-based fluorescence resonance energy transfer (Bv-FRET) assay for measuringmultimerization of cell surface molecules in living cells is described. It has been demonstrated that gonadotropin-releasing hormone recep-tor (GnRH-R) was capable of forming oligomeric com-plexes in the plasma membrane under normal physiological conditions. The mouse gonadotropin-releasing hormone receptor GnRH-R was used to evaluate the efficiency and potential applications of this assay.

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Nội dung Text: Báo cáo khoa học: Development of a baculovirus-based fluorescence resonance energy transfer assay for measuring protein–protein interaction

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