
Báo cáo khoa học: Differential expression of endogenous sialidases of human monocytes during cellular differentiation into macrophages
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Sialidases are enzymes that influence cellular activity by removing terminal sialic acid from glycolipids and glycoproteins. Four genetically distinct sia-lidases have been identified in mammalian cells. In this study, we demon-strate that three of these sialidases, lysosomal Neu1 and Neu4 and plasma membrane-associated Neu3, are expressed in human monocytes. When measured using the artificial substrate 2¢-(4-methylumbelliferyl)-a-d-N-acetylneuraminic acid (4-MU-NANA), sialidase activity of monocytes increased up to 14-fold per milligram of total protein after cells had differ-entiated into macrophages. ...
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