Báo cáo khóa học: Effects of Escherichia coli ribosomal protein S12 mutations on cell-free protein synthesis
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We examined the effects ofEscherichia coliribosomal pro-tein S12 mutations on the efficiency of cell-free protein syn-thesis. By screening 150 spontaneous streptomycin-resistant isolates from E. coliBL21, we successfully obtained seven mutants of the S12 protein, including two streptomycin-dependent mutants. The mutations occurred at Lys42, Lys87, Pro90 and Gly91 of the 30S ribosomal protein S12. We prepared S30 extracts frommutant cells harvested in the mid-log phase.
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