
Báo cáo khoa hoc : Human FAD synthase (isoform 2): a component of the machinery that delivers FAD to apo-flavoproteins
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A soluble form of human FAD synthase (isoform 2; hFADS2) was pro-duced and purified to homogeneity as a recombinant His-tagged protein. The enzyme binds 1 mole of the FAD product very tightly, although non-covalently. Complete release of FAD from the ‘as isolated’ protein requires extensive denaturation.
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