Báo cáo khóa học: Identification of domains on the extrinsic 23 kDa protein possibly involved in electrostatic interaction with the extrinsic 33 kDa protein in spinach photosystem II
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To elucidate the domains on the extrinsic 23 kDa protein involved inelectrostatic interactionwiththeextrinsic33 kDa protein in spinach photosystem II, we modified amino or carboxyl groups ofthe 23 kDa protein to unchargedmethyl ester groups with N-succinimidyl propionate or glycine methyl ester in the presence ofa water-soluble carbodi-imide, respectively.TheN-succinimidyl propionate-modified 23 kDa protein did not bind to the 33 kDa protein associ-atedwithPSIImembranes, whereas the glycinemethyl ester-modified 23 kDa protein completely bound. ...
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