Báo cáo khoa học: Pathways involved in testicular germ cell apoptosis induced by H2O2 in vitro
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The chitosanase fromStreptomycessp. N174 (CsnN174) is an inverting glycoside hydrolase belonging to family 46. Previous studies identified Asp40 as the general base residue. Mutation of Asp40 into glycine revealed an unexpectedly high residual activity. D40G mutation did not affect the stereochemical mechanism of catalysis or the mode of interaction with sub-strate.
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