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Báo cáo khoa học: Probing suggested catalytic domains of glycosyltransferases by site-directed mutagenesis Tobias Hefner and Joachim Stockigt ¨
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The plant enzyme arbutin synthase isolated from cell sus-pension cultures ofRauvolfia serpentinaand heterologously expressed inEscherichia coliis a member of the NRD1b family of glycosyltransferases. This enzyme was used to prove, by site-directed mutagenesis, suggested catalytic domains and reaction mechanisms proposed for enzyme-catalyzed glycosylation. Replacement of amino acids far from the NRD domain do not significantly affect arbutin synthase activity.
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