Báo cáo khoa học: Structural study of the catalytic domain of PKCf using infrared spectroscopy and two-dimensional infrared correlation spectroscopy
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The secondary structure of the catalytic domain from protein kinase Cf was studied using IR spectroscopy. In the presence of the substrate MgATP, there was a significant change in the secondary structure. After heating to 80C, a 14% decrease in thea-helix component was observed, accompanied by a 6% decrease in the b-pleated sheet; no change was observed in the large loops or in 310 -helix plus associated loops.
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